Analysis of the Redox Regulation System and its Application of Molecular breeding in Higher Plants

高等植物氧化还原调控系统分析及其在分子育种中的应用

• 批准号: 15380078
• 负责人: SHIGEOKA Shigeru
• 金额: $ 8.83万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380078/
• 关键词: ascorbate peroxidase; redox regulation; active oxygen species; photooxidative stress; アスコルビン酸ペルオキシダー; アスコルビン酸ペルオキシダーゼ; 選択的スプライシング; 細胞内レドックス制御; SRタンパク質; 過酸化水素; 環境ストレス; 遺伝子発現制御; 転写後調節; アスコルビン酸ペルキオシダーゼ

1)The influence of H_2O_2 accumulation and redox status of the photosynthetic electron transport on the expression of cytosolic ascorbate peroxidase (cAPX).Transgenic tobacco plants expressing catalase and thylakoid-membrane bound APX in chloroplasts, both of which had increased tolerance to photooxidative stress, were utilized for evaluation of the relationship between H_2O_2 accumulation or change of redox status of the PET and cAPX induction under photooxidative stress condition. These results suggest that the induction of cAPX expression is caused by a redox change in the PET, probable PQ pool at an early stage and-thereafter by an increase in the cellular H_2O_2 level.Next, to clarify the mechanism of the decline in oxidative stress tolerance of leaves during the course of senescence, we studied the differences in the levels of various antioxidants and antioxidant enzymes in leaves of different ages.The data suggest that the decline in the levels of antioxidants and antioxidant en … More zymes associated with leaf senescence lead to lower photooxidative-stress tolerance, which might in turn accelerate the propagation of senescence.2)The effect of AOS, signal molecules on the regulation of many physiological processes, including environmental stress responses.We investigated the effect of lowered cAPX activity in transgenic tobacco BY-2 cells, using two transformed BY-2 cell lines, cAPX-S2 and cAPX-S3. Interestingly, the APX-suppressed cells showed different responses and tolerances to environmental stresses, such as heat and salinity. Suppression subtractive hybridization revealed that several heat and salt-stress inducible genes were upregulated in cAPX-S3 cells. HSP70, DnaJ-like protein and purple acid phosphatase were among the constitutively induced genes.3)Molecular breeding of transgenic plants introducing the genes involved in the redox regulation.To identify the genes involved in the the redox regulation in higher plants, we are trying to generate transgenic plants expressing a redox regulatory factor. Less

1）H_2O_2光合电子传输的积累和氧化还原状态对胞质抗坏血酸抗坏血酸过氧化物酶（CAPX）的表达。转基因烟草植物表达过其表达过其表达过氧化氢酶和类囊体 - 甲甲布兰在叶绿体中的APX在叶绿体中的影响，这些植物在叶绿体中均具有对光氧化的影响，_2的含量增加了_2的关系。在光氧化应激条件下，PET和CAPX诱导的氧化还原状态。这些结果表明，CAPX表达的诱导是由PET的氧化还原变化引起的，在早期阶段和以后通过细胞H_2O_2级别的增加而引起的PQ池引起的。下一级别，以阐明敏感过程中的氧化应激耐受性下降的机制，我们研究了各种抗氧化剂和抗氧化剂的差异的差异，我们研究了各种水平的差异。抗氧化剂和抗氧化剂的水平下降……更多与叶片感应相关的酶导致光氧化压力耐受性较低，这可能会加速敏感的传播。2）信号分子的影响。线，CAPX-S2和CAPX-S3。有趣的是，被APX抑制的细胞显示出对环境应力（例如热和盐度）的不同反应和公差。抑制减法杂交表明，在CAPX-S3细胞中，几种热和盐压力诱导的基因上调。 HSP70，DNAJ样蛋白和紫色酸磷酶是组成诱导的基因之一。3）引入氧化还原调节基因的转基因植物的分子繁殖。为了识别较高植物中氧化还原调控的基因，我们正在努力产生表达氧化还原原料调节因子的转基因植物。较少的

项目成果

Kobayashi, D., et al.: "Molecular characterization and redox regulation of phosphoribulokinase from the cyanobacterium Synechococcus PCC7942"Plant and Cell physiology. 44. 269-276 (2003)

Kobayashi, D. 等人：“蓝细菌聚球藻 PCC7942 磷酸核糖激酶的分子特征和氧化还原调节”植物和细胞生理学。

Yoshimura, K., et al.: "Enhancement of stress tolerance in transgenic tobacco plants overexpressing Chlamydomonas glutathione peroxidase in chloroplasts or cytosol"The Plant Journal. 37. 12-33 (2004)

Yoshimura，K.等人：“在叶绿体或细胞质中过度表达衣藻谷胱甘肽过氧化物酶的转基因烟草植物中胁迫耐受性的增强”植物杂志。

Madhusudhan, R., et al.: "Characterization of an ascorbate peroxidese in plastids of tobacco BY-2 cells"Physiologia Plantarum. 117. 550-557 (2003)

Madhusudhan, R. 等人：“烟草 BY-2 细胞质体中抗坏血酸过氧化物酶的表征”Physiologia Plantarum。