Development and Application of High Performance Capillary Electrophoresis Using a New Capillary Fabricated by Laser

激光制作的新型毛细管高性能毛细管电泳的开发与应用

• 批准号: 15350045
• 负责人: KANETA Takashi
• 金额: $ 6.85万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15350045/
• 关键词: Laser-fabricated capillary; Capillary electrophoresis; Hadamard transformation; Electrokinetic injection; Multiple sample injection; Laser-induced fluorescence; Post-column reaction; Trace Analysis; 光学ゲート法; 蛍光検出; レーザー励起蛍光検出; タンパク質; イムノアッセイ; 光

A novel capillary fabricated by laser ablation was developed and applied to Hadamard transform capillary electrophoresis and post-column reaction for fluorescence detection in capillary electrophoresis. A small hole was fabricated at the center of the capillary. The small hole functions as an inlet port for the sample and labeling reagent in multiple sample injection and post-labeling reaction, respectively. In multiple sample injection, the hole is immersed in a sample solution and the end of the capillary that is usually employed for sample introduction is immersed in a buffer solution. A high potential is continuously applied between the injection port and the end of the capillary, which allows the sample solution to be introduced into the separation capillary. By application of a higher potential modulated according to a Hadamard code between both ends of the capillary, the buffer solution is injected into the separation capillary. This method was successfully employed for multiple sample injection in Hadamard transform capillary electrophoresis equipped with an absorbance detector and a laser-induced fluorescence detector. In post-column reaction, the hole was made at upstream of a fluorescence detector. The hole is immersed in a labeling reagent solution. Analyte zones separated by capillary electrophoresis were mixed with the labeling reagent introduced from the hole, followed by the reaction of the labeling reagent with analytes and fluorescence detection of the labeled analytes.

开发了一种由激光消融制造的新型毛细管，并应用于Hadamard转化毛细管电泳和柱后柱反应，以用于毛细管电泳中的荧光检测。在毛细管的中心制造了一个小孔。小孔分别充当样品的入口端口，并分别在多种样品注射和标记后反应中标记试剂。在多次样品注入中，将孔浸入样品溶液中，并且通常用于样品引入的毛细管末端被浸入缓冲溶液中。在注入端口和毛细管末端之间连续地施加高电位，这使得样品溶液可以引入分离毛细管中。通过根据毛细管两端之间的HADAMARD代码对更高的电势调制的应用，将缓冲溶液注入分离毛细管中。该方法已成功地用于装有吸光度检测器和激光诱导的荧光检测器的Hadamard Transform毛细管电泳中的多次样品注射。在柱后反应中，该孔是在荧光检测器上游的。该孔浸入标记试剂溶液中。将通过毛细管电泳分离的分析区与从孔中引入的标记试剂混合，然后将标记试剂与分析物和标记分析物的荧光检测进行反应。

项目成果

Suppression of electroosmotic flow and its application to determination of electrophoretic mobilities in a poly(vinylpyrrolidone)-coated capillary

• DOI: 10.1016/j.chroma.2005.08.062
• 发表时间: 2006-02-17
• 期刊: JOURNAL OF CHROMATOGRAPHY A
• 影响因子: 4.1
• 作者: Kaneta, T;Ueda, T;Imasaka, T
• 通讯作者: Imasaka, T

On-Line Concentration of a Protein Using Denaturation by Sodium Dodecyl Sulfate

• DOI: 10.2116/analsci.21.37
• 发表时间: 2005-01
• 期刊: Analytical Sciences
• 影响因子: 1.6
• 作者: P. Jing;T. Kaneta;T. Imasaka

金田隆, 西田未来, 今坂藤太郎: "キャピラリー電気泳動によるキラル分離へのアダマール変換法の応用"分析化学. 52. 1193-1197 (2003)

Takashi Kaneda、Miki Nishida、Totaro Imasaka：“Hadamard 变换方法在毛细管电泳手性分离中的应用”《分析化学》52. 1193-1197 (2003)。

Absorption Spectrometry Using a Diffractive Optical Element Constructed by Gel Electrophoresis

使用凝胶电泳构建的衍射光学元件进行吸收光谱测定

• 发表时间: 2003
• 期刊: Analytical Sciences 19
• 作者: Ayako Shigyo;Shinichi Miki;Takashi Kaneta;Totaro Imasaka
• 通讯作者: Totaro Imasaka

Kazuki Hata, Yasuhiko Kichise, Takashi Kaneta, Totaro Imasaka: "Hadamard Transform Microchip Electrophoresis Combined with Diode Laser Fluorometry"Analytical Chemistry. 75. 1765-1768 (2003)

Kazuki Hata、Yasuhiko Kichise、Takashi Kaneta、Totaro Imasaka：“Hadamard 变换微芯片电泳与二极管激光荧光法相结合”分析化学。