Laser Micromanipulation Using Microtools at Micro Fluidic Environment

在微流体环境下使用微型工具进行激光显微操作

基本信息

批准号：
14550233
负责人：
ARAI Fumihito
金额：
$ 2.24万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

(I)Design and Fabrication of Microtools(1)Utilization of adherent microtools : We adhered the yeast cell and succeeded in transportation of it without direct laser irradiation to the target cell.(2)Fabrication of microtools : Using photoclosslinkable resin, we made several microtools, such as fluorescent microtool, colored microtool, and functional microtools by assembling microbeads.(3)Manipulation of microorganism using microtools : A microorganism was immobilized on the microtool, and was manipulated indirectly by trapping the microtool made of photoclosslinkable resin in high speed.(II)Preparation of Microtools and Environmental Control(1)Method of preparation of microtools : We fabricated microtools inside the chip by adjusting the density of photoclosslinkable resin and irradiation of UV light.(2)Design and fabrication of micro fluidic environment : We made a micro-chip and immobilized cells in it. The fluid environment was controlled for measurement of cell reaction. We performe … More d experiments of culture and fluorescent reaction of yeast cells. A single cell analysis was possible with this technology.(3)Control of fluid condition in the micro-channel : We designed and fabricated a micro-calve using thermo sensitive hydro gel, which is gelled by heating. The flow condition in the micro-channel was controlled easily.(III)Stabilization of Position and Orientation Control of Target Object Using Microtools(1)Stable manipulation by laser micromanipulation : We derived stability condition of the multiple microtools along each given trajectories. Effect of the Brownian motion was considered in the analysis. We succeeded in trajectory control of multiple targets stably. We also measured the optical trap force. Effectiveness of the proposed trajectory control method was confirmed through the experiments.(2)Position and orientation control by laser micromanipulation : We assembled three microbead in triangular shape and controlled its position and orientation independently. Less

(I)微型工具的设计和制造(1)粘附微型工具的利用：我们粘附酵母细胞并成功地运输它，而无需直接激光照射到目标细胞。(2)微型工具的制造：使用可光致交联的树脂，我们制作了几个微型工具，如荧光微型工具、彩色微型工具和通过组装微珠而形成的功能性微型工具。(3)使用微型工具操纵微生物：A将微生物固定在微型工具上，并通过高速捕获由可光致交联树脂制成的微型工具来间接操纵微生物。（二）微型工具的制备和环境控制（1）微型工具的制备方法：我们通过调整芯片内部的微型工具来制造微型工具。 (2)微流体环境的设计和制造：我们制作了微芯片并固定化了细胞控制流体环境以测量细胞反应。使用该技术可以进行单细胞分析。 (3) 控制微通道中的流体条件：我们利用热敏水凝胶设计并制作了微型通道，通过加热使其凝胶化，微通道中的流动条件很容易控制。(三)使用微型工具实现目标物体的位置和方向控制的稳定(1)稳定。激光操纵微操纵：我们推导了多个微型工具沿每个给定轨迹的稳定性条件，在分析中我们成功地实现了多个目标的轨迹控制。我们还测量了所提出的轨迹控制的有效性。 (2)激光显微操作的位置和方向控制：我们将三个微珠组装成三角形，并独立控制其位置和方向。