Planarity Recognition of Polycyclic Aromatic Hydrocarbons in Supercritical Fluid Chromatography

超临界流体色谱中多环芳烃的平面识别

• 批准号: 02650540
• 负责人: JINNO Kiyokatsu
• 金额: $ 1.22万
• 依托单位: Toyohashi University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02650540/
• 关键词: supercritical fluid chromatography; polycyclic aromatic hydrocarbons; planarity recognition; 超臨界流体クロマトグラフィ; 超臨界流体クロマトグラフィ(SFC); 多環芳香族化合物(PAH)

Selectivity is one of the most important variables to achieve the separation of structually related compounds for identification and quantitation. In Liquid chromatography (LC), selectivity can be controlled by changing the mobile phase composition, the column temperature and/or the type of stationary phases. It is possible to use solvent systems to change selectivity in LC. However, the choice of mobile phases in supercritical fluid chromatography (SFC) is very limited ; the most popular mobile phase is carbon dioxide, into which modifiers are sometimes added. Large changes in selectivity will not be expected, although it is feasible to modify the selectivity by changing the state variables such as temperature and pressure and adding mofifiers. The most effective approach to attain the desiable selectivity in SFC is to alter the type of stationary phases. With packed capillary SFC, various types of stationary phases which offer different selectivity in LC can be utilised. The main pur … More pose of this investigation is to examine the difference in selectivity between LC and SFC using identical stationary phases, especially focusing on their ability to recognize the difference in molecular planarity of polycyclic aromatic hydrocarbons. Then new stationary phases which have different selectivity in LC will be examined in SFC in order to know the basic difference between LC and SFC. As the results, we have found that liquid-crystal bonded phase and cyclodextrin bonded phase have better selectivity in molecular planarity recognition than those in LC, because in SFC mobile phase solvation effect to the solute-stationary phase interaction is smaller than that in LC situation and therefore almost simple molecular-molecular interaction is possible to make in chromatographic environment. The design of new stationary phases based on molecular-molecular interaction can be easily possible to evaluate in SFC, rather than LC conditions. Then the resulted information can be useful to predict the actual performance of such stationary phases in LC. The scheme of designing new stationary phases which can give us better of higher selectivity for particular separations is as follows : design the molecular-molecular interaction, consider the stationary phase structure based on the interaction, synthesize the phase, evaluate the performance by packed column SFC and then apply it in LC. The results of this investigation can clearly indicate this scheme is one of the best way to obtain highly selective stationary phases in LC. Less

选择性是实现结构相关化合物分离以进行鉴定和定量的最重要变量之一。在液相色谱 (LC) 中，可以通过改变流动相组成、柱温和/或固定相类型来控制选择性。可以使用溶剂系统来改变 LC 的选择性，但是，超临界流体色谱 (SFC) 中流动相的选择非常有限；最常用的流动相是二氧化碳，有时会添加改性剂。尽管可以通过改变温度和压力等状态变量以及添加改性剂来改变选择性，但预计选择性不会发生大的变化。在 SFC 中获得所需选择性的最有效方法是改变固定相的类型。对于填充毛细管 SFC，可以使用在 LC 中提供不同选择性的各种类型的固定相。本研究的主要目的是检查使用相同固定相的 LC 和 SFC 之间的选择性差异。特别关注它们识别多环芳烃分子平面性差异的能力，然后在 SFC 中检查在 LC 中具有不同选择性的新固定相，以了解 LC 和 SFC 之间的基本区别。发现液晶键合相和环糊精键合相在分子平面识别方面比LC具有更好的选择性，因为在SFC中流动相溶剂化对溶质-固定相相互作用的影响小于LC在 LC 情况下，因此可以在色谱环境中进行几乎简单的分子-分子相互作用，可以在 SFC（而不是 LC 条件）中轻松评估基于分子-分子相互作用的新固定相。可用于预测 LC 中此类固定相的实际性能 设计新固定相的方案可以为我们提供更好或更高的特定分离选择性：设计分子-分子相互作用，考虑固定相结构。基于相互作用，合成相，通过填充柱SFC评估性能，然后将其应用于LC。本次研究的结果可以清楚地表明该方案是在LC中获得高选择性固定相的最佳方法之一。