Solvent-less Analytical Method for Environmental Analysis

用于环境分析的无溶剂分析方法

• 批准号: 08640771
• 负责人: JINNO Kiyokatsu
• 金额: $ 1.66万
• 依托单位: TOYOHASHI UNIVERSITY OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640771/
• 关键词: SPME; HPLC; solventless analytical method for environmental analysis

In this project the following results were obtained :1)Basic evaluation of SPMESPME which is a totally different from the present sample extraction method, is a method to extract some compounds from the matrix by the surface coating (usually polymeric material) on fused-silica capillary and concentrate those compounds in the coating material. The basic key point in SPME technique is what kind of coating materials will be the most suitable for the analytical purposes, for example, for pesticides analysis in environmental samples, choosing the appropriate coating of the fiber surface and then using such coating fiber as a tool to establish the extraction conditions are the important. In this project the best SPME conditions were established by using polyacryrate coating as the extraction medium.2) Basic evaluation of microcolumn LC (Capillary LC)The scparation method which is most suitable to combine to SPME extraction method is the technique so-called microcolumn LC(Capillary LC), becau … More se both techniques do require very small amount of organic solvents as the extraction medium and the mobile phase, respectively. For microcolumn LC the separation conditions should be different from that with conventional column LC and therefore we have needed to establish the best separation conditions for pesticides analysis in water samples. In this project 1 mm i.d. microcolumn was recommended as the tool for actual sample analysis.3) Interfacing between SPME and microcolumn LCIn order to interface SPME to microcolumn LC,the device specially designed is required. In this work we have developed the device and applied it to the interfacing between SPME and microcolumn LC.The required amount of organic solvents for the extraction process is less than 30 uL.Since the typical mobile phase flow rate is 50 uLomin for microcolumn LC,if the analysis time is 60 minutes, 3 mL solvent is required and total solvents consumption is about less than 3.1 mL.To reduce the consumption more, one can use much smaller diameter columns for the separation but the interfectly device should be more complicated because the dead volume should be perfectly reduced.4) Application of SPME/microcolumn LCAs the application of this technique pesticides analysis in environmental water samples and drug analysis in human urine samples were successfully performed. Less

在该项目中获得了以下结果：1）SPMESPME的基本评价，它是一种与现有样品提取方法完全不同的方法，是一种通过熔融石英上的表面涂层（通常是聚合物材料）从基质中提取某些化合物的方法SPME 技术的基本要点是哪种涂层材料最适合分析，例如，对于环境样品中的农药分析，选择合适的纤维表面涂层。然后使用这样的以聚丙烯酸酯涂层为萃取介质，建立最佳的固相微萃取条件是重要的。2）微柱LC（毛细管LC）的基本评价最合适的分离方法。与SPME萃取法相结合的是所谓的微柱LC（毛细管LC）技术，因为这两种技术确实需要分别非常少量的有机溶剂作为萃取介质和流动相。对于微柱液相色谱，分离条件应与传统柱液相色谱不同，因此我们需要建立水样中农药分析的最佳分离条件，在该项目中建议使用 1 mm 内径的微柱作为实际样品分析的工具。 3）SPME和微柱LC之间的接口为了将SPME连接到微柱LC，需要专门设计的装置，在这项工作中我们开发了该装置并将其应用于SPME和微柱LC之间的接口。微柱LC。萃取过程所需的有机溶剂量小于30 uL。由于微柱LC的典型流动相流速为50 uLomin，如果分析时间为60分钟，则需要3 mL溶剂，总溶剂消耗量大约小于3.1 mL。为了进一步减少消耗，可以使用更小直径的柱进行分离，但交叉装置应该更复杂，因为死体积应该完美4) SPME/微柱LCAs的应用成功地应用了该技术在环境水样中的农药分析和人体尿液样品中的药物分析。