Analysis of gene and protein expression on infiltrating lymphocytes

浸润淋巴细胞基因和蛋白表达分析

• 批准号: 14370555
• 负责人: MOCHIZUKI Manabu
• 金额: $ 9.41万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14370555/
• 关键词: sarcoidosis; Vogt-Koyanagi-Harada disease; T cell receptor; γδ lymphocytes; DNAチップ; ぶどう膜炎; T細胞クローン; DNA解析

Expression profile of gene and protein on infiltrating lymphocytes in the eye was investigated using T cell clones established from infiltrating cells in the eye of patients with various clinical entities of ocular inflammatory disorders, uveitis.Ocular infiltrating cells in patients with sarcoidosis and patients with Vogt-Koyanagi-Harada disease were compared each other. There were only four genes which were three times stronger in sarcoidosis than in Vogt-Koyanagi-Harada disease. On the other hand, in Vogt-Koyanagi-Harada disease, 72 genes were expressed three times stronger than in sarcoidosis. In sarcoidosis, T cell receptor αchain expression was 3.4 times higher than Vogt-Koyanagi-Harada disease, while in Vogt-Koyanagi-Harada disease T cell receptor δ chain and soluble δ chain were 6.2 times and 5.4 times higher than sarcoidosis, respectively. These data suggest that subset of infiltrating lymphocytes in the eye was completely different in the two diseases : ocular infiltrating ly … More mphocytes were αβ lymphocytes in sarcoidosis whereas γδ lymphocytes in Vogt-Koyanagi-Harada disease. It is generally understood that γδ lymphocytes consist of 1-5% of T lymphocytes in the body and present mainly in the skin and epithelium of the mucosa. They play an important role in the innate immunity. It has not reported previously that γδ lymphocytes infiltrate in the eye. This study is the first to report that γδ lymphocytes infiltrate in the eye of patients with Vogt-Koyanagi-Harada disease.Vogt-Koyanagi-Harada disease is an autoimmune disease specific to melanocytes. Melanocyte existing both in the eye and the skin are the targets of autoimmune reactions by T lymphocytes including γδ lymphocytes. The γδ lymphocytes found in the eye of Vogt-Koyanagi-Harada disease might be related to γδ lymphocytes in the skin. It is suggested that immunopathogenic mechanisms of Vogt-Koyanagi-Harada disease might be a cross reaction and immunological recognition by the γδ lymphocytes against melanocytes. Less

基因和蛋白质在眼睛浸润性淋巴细胞上的表达能力使用来自眼部炎症性疾病的各种临床实体，葡萄膜炎的患者的眼睛中的浸润细胞中建立的T细胞克隆。在结节病中只有四个基因比Vogt-Koyanagi-Harada疾病高三倍。另一方面，在Vogt-Koyanagi-Harada疾病中，72个基因的表达比结节病高三倍。在结节病中，T细胞受体α链表达比Vogt-koyanagi-Harada疾病高3.4倍，而在Vogt-Koyanagi-Harada疾病中，T细胞受体δ链和固体δ链分别分别比在结节症中高6.2倍和5.4倍。这些数据表明，在两种疾病中，眼睛中浸润性淋巴细胞的子集完全不同：眼部浸润……在结节病中，更多的mphocytes是αβ淋巴细胞，而Vogt-Koyanagi-Harada病中的γδ淋巴细胞。通常可以理解，γδ淋巴细胞由体内1-5％的T淋巴细胞组成，主要存在于粘膜的皮肤和上皮中。他们在先天免疫中起着重要作用。以前尚未报道γδ淋巴细胞在眼中浸润。这项研究是第一个报告说，Vogt-Koyanagi-Harada疾病患者眼中γδ淋巴细胞浸润。 Vogt-Koyanagi-Harada疾病是一种特有黑素细胞的自身免疫性疾病。眼睛和皮肤中存在的黑素细胞是包括γδ淋巴细胞在内的T淋巴细胞自身免疫反应的靶标。在Vogt-Koyanagi-Harada疾病眼中发现的淋巴细胞可能与皮肤中的γδ淋巴细胞有关。有人提出，Vogt-Koyanagi-Harada疾病的免疫致病机制可能是针对黑素细胞的γδ淋巴细胞的交叉反应和免疫学识别。较少的

项目成果

Microdissection and gene rearrangement analysis of paraffin-embedded specimens of orbital malignant lymphoma.

眼眶恶性淋巴瘤石蜡包埋标本的显微切割和基因重排分析。

• DOI: 10.1007/s10384-003-0038-7
• 发表时间: 2004
• 期刊: Japanese journal of ophthalmology
• 影响因子: 2.4
• 作者: Miyanaga,Masaru;Kiyosawa,Motohiro;Takase,Hiroshi;Eishi,Yoshinobu;Shen,DeFen;Chan,Chi-Chao;Mochizuki,Manabu
• 通讯作者: Mochizuki,Manabu

Takase H, et al.: "The presence of macrophage migration inhibitory factor in human trabecular meshwork and its upregulatory effects on the T helper 1 cytokine"Investigative Ophthalmology & Visual Science. 43・8. 2691-2696 (2002)

Takase H 等人：“人小梁网中巨噬细胞迁移抑制因子的存在及其对 T 辅助细胞 1 细胞因子的上调作用”调查眼科与视觉科学 43·8（2002）。

CTLA-4^+CD8^+ T cells that encounter B7-2+ iris pigment epithelial cells express their own B7-2 to achieve global suppression of T cell activation^1.

遇到 B7-2 虹膜色素上皮细胞的 CTLA-4^ CD8^ T 细胞表达自己的 B7-2，以实现 T 细胞激活的全局抑制^1。

• 发表时间: 2004
• 期刊: The Journal of Immunology. 172
• 作者: Sugita Sunao;Tat Fong Ng;Johannnes Schwartzkopff;J.Wayne Streilein.
• 通讯作者: J.Wayne Streilein.

Capasity of ocular infiltraring T helper type 1 cells of patients with noninfectious to produce chemokines.

非感染性患者眼部浸润的 1 型辅助 T 细胞产生趋化因子的能力。

• 期刊: Br J Ophthalmol (In press)
• 作者: Takase H;Sugita S;Taguchi C;Imai Y;Mochizuki M.
• 通讯作者: Mochizuki M.

The presence of macrophage migration inhibitory factor in human trabecular meshwork and its upregulatory effects on the T helper 1 cytokine.

人小梁网中巨噬细胞迁移抑制因子的存在及其对 T 辅助细胞 1 细胞因子的上调作用。

• 发表时间: 2002
• 期刊: Investigative ophthalmology & visual science
• 影响因子: 4.4
• 作者: Takase,Hiroshi;Sugita,Sunao;Rhee,DouglasJ;Imai,Yasuhisa;Taguchi,Chikako;Sugamoto,Yoshiharu;Tagawa,Yoshitsugu;Nishihira,Jun;Russell,Paul;Mochizuki,Manabu
• 通讯作者: Mochizuki,Manabu