Clinical and basic research on the pathogenic mechanisms of HTLV-I uveitis

HTLV-I葡萄膜炎发病机制的临床及基础研究

• 批准号: 06454501
• 负责人: MOCHIZUKI Manabu
• 金额: $ 4.67万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454501/
• 关键词: HTLV-I; uveitis; T cell clone; viral load; cytokine; HTLV-I infected lymphocyte; HTLV-Iぶどう膜炎; RT-PCR; 定量的PCR; virus load

To investigate the pathogenic mechanisms of HTLV-I uveitis (HU), immunological and molecular biological studies were carried out using infiltrating cells in the eye and peripheral blood monocytes (PBM) of patients with HU.The proviral DNA of HTLV-I was detected in the ocular fluid of HU patients by polymerase chain reaction (PCR), and the gene expression of HTLV-I was detected from the ocular infiltrating cells at mRNA level by RT-PCR.In addition, the gene expression of cytokines (IL-1alpha, IL-2, IL-3, IL-4, IL-6, IL-8, IL-10, TNF-alpha, IFN-gamma and GM-CSF) was examined by RT-PCR and the mRNA of IL-6 was detected from ocular infiltrating cells. T cell clones (TCC) were established from ocular infiltrating cells and PBM of HU patients by limiting dilution methods. HTLV-I proviral DNA (+) TCC expressed HTLV-I associated proteins and viral particles were found in TCC from ocular fluid by electron microscopic examination. The TCC from ocular fluid and PBMC produced IL-1alpha, IL-6, IL-8, TNF-alpha, IFN-gamma and GM-CSF.These findings suggest that HTLV-I infected cells are presented in the eye of HU patients and the HTLV-I infected cells produce a variety of cytokines which modulate the intraocular inflammation.

为了研究HTLV-I葡萄膜炎（HU）的致病机制，使用眼睛和外周血单核细胞（PBM）进行了HU的患者的HTLV-I临时DNA，在HTLV-I的p cressention and Plyymerid Repande（PR）中，使用了HTLV-I的临床动物DNA，并通过HU的基因持续化（PBM）进行了peccr酶的基因，并通过HU的基因持续化（PBM）进行了PRONIDS键反应（PBM），进行了HTLV-I的PRONIMSASE PREMSSASE PRONMERSASE PRONMERID RESSINAL PRONMERSRID RESSISTINAL和外周血单核细胞（PBM），进行了免疫学和分子生物学研究。通过rt-pcr.中的RT-PCR.在另外从眼部浸润细胞中检测到HTLV-I，并在基因表达（IL-1Alpha，IL-2，IL-3，IL-3，IL-4，IL-4，IL-6，IL-6，IL-8，IL-8，IL-8，IL-10，IL-10，IL-10，IL-10，TNF-ALPHA，TNF-ALPHA，TNF-ALPHA，IFN-GMMA和GM-CSF）中均被检验。眼部浸润细胞。通过限制稀释方法，从HU患者的眼部浸润细胞和PBM建立了T细胞克隆（TCC）。通过电子显微镜检查，通过眼部流体发现了HTLV-I前病毒DNA（+）TCC表达的HTLV-I相关蛋白和病毒颗粒。眼部流体和PBMC的TCC产生了IL-1Alpha，IL-6，IL-8，TNF-Alpha，IFN-GAMMA和GM-CSF。这些发现表明，HTLV-I感染细胞在HU HU患者的眼睛中呈现出来，并在HTLV-I的细胞中产生各种cytoction cytoction cytoction intaiment in Intaiment in Intaiment。

项目成果

Kazuhiro Masuoka: "Polyclonal use of T-cell receptor α for human T-cell lymphotropic virus type 1-infected T cells" Invest Ophthalmol Vis Sci. 36. 254-258 (1995)

Kazuhiro Masuoka：“T 细胞受体 α 在人类 T 细胞嗜淋巴细胞病毒 1 型感染的 T 细胞中的多克隆用途”Invest Ophasemol Vis Sci 36. 254-258 (1995)

Ayako Ono, Tomoyuki Miura, Shinji Araki, Kazunari Yamaguchi, Kiyoshi Takatsuki, Shigeo Mori, Masanori Hayami, Manabu Mochizuki, Toshiki Watanabe: "Subtype analysis of HTLV-1 in patients with HTLV-1 uveitis." Japanese Journal of Cancer Research. 85. 767-77

Ayako Ono、Tomoyuki Miura、Shinji Araki、Kazunari Yamaguchi、Kiyoshi Takatsuki、Shigeo Mori、Masanori Hayami、Manabu Mochizuki、Toshiki Watanabe：“HTLV-1 葡萄膜炎患者中 HTLV-1 的亚型分析。”

Kumiko Nakao, Norio Ohba, Hajime Inomata, Masahiro Yamamoto, Kenji Ohshima, Hideyuki Hayashi, Shinobu Akiya, Kenji Ibi, Manabu Mochizuki, Koichi Yoshimura, Eiko Ikeda, Kazuo Nakatsuka, Hiromi Ono, Shinji Oono, Eiichi Shimada, Ryoichi Okamura, Koichiro Ina

中尾久美子、大场纪夫、猪又一、山本雅宏、大岛贤二、林英幸、秋谷忍、伊比健二、望月学、吉村浩一、池田英子、中冢一夫、小野弘美、大野真司、岛田英一、冈村亮一、伊那晃一郎

Kazunari Yamaguchi: "Human Tlymphotropic virus type 1 uveitis after Graves' disease" Br.J.Ophthalmol.78. 163-166 (1994)

Kazunari Yamaguchi：“格雷夫斯病后人类嗜淋巴细胞病毒 1 型葡萄膜炎”Br.J.Ophasemol.78。

Kazunari Yamaguchi: "Human T-lymphotropic virus type I uveitis" Leukemia. 8(Suppl). 88-89 (1994)

Kazunari Yamaguchi：“人类 T 淋巴细胞病毒 I 型葡萄膜炎”白血病。