Involvement of annexin 5 in G protein mediated signal transduction

膜联蛋白 5 参与 G 蛋白介导的信号转导

基本信息

批准号：
14360181
负责人：
KAWAMINAMI Mitsumori
金额：
$ 7.62万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14360181/
关键词：
G protein annexin 5 GnRH LH βsubunit pituitary gonadotrope corpus luteum testis プロラクチン

项目摘要

A new G protein mediated signal transduction mechanism was studied. It was clarified that annexin 5 took part in the G protein coupled signal transduction process. Annexin is a member of newly identified calcium-phospholipid binding proteins. The outline of the results is brought together as follows.1.From GnRH receptor to annexin 5 synthesisIt was clarified that the MAPK system lay between the GnRH receptor and annexin 5 mRNA synthesis in the pituitary gonadotropes. In addition, it was observed that annexin 5 was indispensable to the process that GnRH promotes the gonadotrophin secretion, and clarified that annexin 5 was a new signal factor of the GnRH receptor signal transduction.2.Annexin 5 in the ovaryChanges in ovarian annexin 5 were analyzed. The synthesis of annexin 5 was demonstrated to start after luteinization and the expression of annexin 5 was inhibited by prolactin. It was clarified that annexin 5 appeared in corpus luteum by the inhibition of prolactin secretion and those cells were in apoptosis. In addition, it was clarified that local GnRH enhanced annexin 5 expression and induced apoptosis.3.Annexin 5 in the testisAnnexin 5 was demonstrated to distribute to various testicular cells. Among these, endotherial cells and Leydig dells were intensely stained with anti-annexin 5. It was clarified that annexin 5 of Leydig cells was increased by the administration of human chorinic gonadotropin. This effect was shown to be mediated by the local GnRH action.Getting together, annexin 5 was shown to be under the control of GnRH receptor in the anterior pituitary gonadotropes, luteal cells and Leydig cells. Annexin 5 is a biomarker of GnRH receptor. GnRH receptor-annexin 5 system seems to be utilized in various cell species.

研究了一种新的G蛋白介导的信号转导机制。可以澄清说，膜联蛋白5参加了G蛋白偶联的信号转导过程。膜联蛋白是新鉴定的钙磷脂结合蛋白的成员。结果的轮廓如下。1。从GnRH受体到膜联蛋白5合成的概述，澄清了MAPK系统位于垂体性腺泡中的GnRH受体和Annexin 5 mRNA合成之间。此外，观察到膜联蛋白5对于GNRH促进促性腺营养蛋白分泌是必不可少的，并澄清了膜联蛋白5是GnRH受体信号转导的新信号因子。2.在卵巢蛋白5中的Ovarychanges中，分析了卵巢蛋白5的Annexin 5。证明膜联蛋白5的合成是在黄体化后开始的，催乳素抑制了膜联蛋白5的表达。可以澄清说，通过抑制催乳素分泌，膜联蛋白5出现在Luteum中，而这些细胞处于凋亡。此外，澄清说，局部GNRH增强了膜联蛋白5的表达和诱导的凋亡。3.Annexin5在睾丸蛋白5中5中被证明分布在各种睾丸细胞上。其中，抗动脉蛋白5对触觉细胞和Leydig dells进行了严格染色。澄清说，通过施用人类绒毛膜促性腺激素，可以增加leydig细胞的膜联蛋白5。这种作用被证明是由局部GNRH动作介导的。共同结合，膜联蛋白5被证明在垂体前促性腺促性腺体，黄体细胞和leydig细胞中的GnRH受体的控制下。膜联蛋白5是GNRH受体的生物标志物。 GnRH受体 - 纳米5系统似乎在各种细胞物种中使用。

项目成果

期刊论文数量（48）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Chromosomal mapping and zygosity check of transgenes based on flanking genome sequences determined by genomic walking

DOI：
10.1538/expanim.53.103
发表时间：
2004-04-01
期刊：
EXPERIMENTAL ANIMALS
影响因子：
2.4
作者：
Noguchi, A;Takekawa, N;Suzuki, O
通讯作者：
Suzuki, O

Chemical chaperone therapy for brain pathology in GM1-gangliosidosis

DOI：
10.1073/pnas.2536657100
发表时间：
2003-12-23
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Matsuda, J;Suzuki, O;Suzuki, Y
通讯作者：
Suzuki, Y

Suzuki O: "Optimization of superovulation induction by human menopausal gonadotropin in guinea pigs based on follicular waves and FSH-receptor homologies."Mol Reprod Dev. 64. 219-225 (2003)

Suzuki O：“基于卵泡波和 FSH 受体同源性，优化豚鼠中人绝经期促性腺激素诱导超数排卵。”Mol Reprod Dev。