Recovery from severe hearing impairment by regeneration of inner ear sensory organs

通过内耳感觉器官再生从严重听力障碍中恢复

基本信息

批准号：
14207068
负责人：
ITO Juichi
金额：
$ 31.62万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14207068/
关键词：
hearing impairment regeneratio transplantation inner ear hair cell spiral ganglion cell stem cell nerve growth factor 高度感音難聴ラセン神経節細胞内耳感覚細胞機能回復神経幹細胞聴神経

项目摘要

Our final goal is to establish a therapeutic strategy for inner ear diseases, especially for severe sensorineural hearing impairment. There are more than 3 hundred thousand deaf or highly hearing impaired people in Japan. To recover hearing ability of those deaf people has been one of the most important themes in the field of Otolaryngology. Dysfunctions of several regions in the cochlea, such as spiral ganglion cells, sensory hair cells, and cochlear wall, cause hearing disability. Among them dysfunction of sensory hair cells and the spiral ganglion cells are the most important factors. If the hair cells or spiral ganglion cells regenerate and recover function, hearing ability may recover.The first experiments have been attempted in the auditory system, involving repair to the central auditory pathway using rats. Embryonic brain tissue was placed into a lesion in the ventral cochlear tract, resulting the regeneration of the ventral cochlear tract and associated recovery of the ABR.Then, we examined the potential of transplantation approaches for inner ear diseases. The targets for transplantation approaches included sensory hair cells(HCs) and spiral ganglions(SGs). Mice and rats were used as experimental animals. Embryonic stem cells(ES), Neural stem(NS) cells and mesenchymal stem(MS) cells were used as donors. Cell transplantation into the inner ear was performed through the round window, cochlear lateral wall, or semicircular canal. These donor cells survived in various regions of the inner ear including the sensory epithelia. This indicates that transplantation approach can be utilized for protection of HCs and SGs from cell death. Consequently, transplantation approaches have the potential for treatment of inner ear diseases

我们的最终目标是建立内耳疾病的治疗策略，尤其是对于严重的感觉性听力障碍。日本有超过30万聋哑人或高度听力受损的人。恢复这些聋人的听力能力一直是耳鼻喉科领域中最重要的主题之一。耳蜗中几个区域的功能障碍，例如螺旋神经节细胞，感觉毛细胞和耳蜗壁，会导致听力障碍。其中最重要的因素是感觉毛细胞和螺旋神经节细胞的功能障碍。如果毛细胞或螺旋神经节细胞再生并恢复功能，则可以恢复听力。将胚胎脑组织放入腹侧人工耳蜗中的病变中，导致腹侧耳蜗的再生以及ABR的相关恢复。然后，我们检查了内耳疾病的移植方法的潜力。移植方法的目标包括感觉毛细胞（HCS）和螺旋神经节（SGS）。小鼠和大鼠用作实验动物。胚胎干细胞（ES），神经干细胞（NS）细胞和间质干细胞（MS）细胞用作供体。通过圆形窗户，人工耳蜗或半圆形管进行细胞移植到内耳。这些供体细胞在内耳的各个区域中幸存，包括感觉上皮。这表明可以将移植方法用于保护HC和SGS免受细胞死亡的影响。因此，移植方法具有治疗内耳疾病的潜力