Development of the regeneration method for inner ear disorders -Applying molecular mechanisms of inner ear development-

内耳疾病再生方法的开发 -应用内耳发育的分子机制-

• 批准号: 20249068
• 负责人: ITO Juichi
• 金额: $ 30.12万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20249068/
• 关键词: 再生医学; 発生学; 細胞増殖; 転写因子; 幹細胞; 支持細胞; 有毛細胞

In this research we found several factors that are important for inner ear development contributed to regenerative medicine of inner ears. Silencing of p27Kip1, cell cycle dependent kinase inhibitor, caused proliferation of postnatal mammalian cochlear supporting cell that had been considered to be non-proliferative. HGF and EP4 agonist protected or treated inner ear disorders including noise induced hearing loss and hearing loss due to aminoglycoside treatment. We also identified downstream mechanisms of how these molecules affect on inner ear hair cells. In addition novel roles of Notch signaling in inner ear development. Notch signaling not only specifies cell fates between inner ear hair and supporting cells but also regulates timing of differentiation of sensory epithelial progenitors and maintains those cell population.

在这项研究中，我们发现几个对内耳发育很重要的因素有助于内耳的再生医学。 P27KIP1的沉默，细胞循环依赖性激酶抑制剂导致产后哺乳动物的耳蜗支撑细胞的增殖，该细胞被认为是非增生性的。 HGF和EP4激动剂受保护或处理过的内耳性疾病，包括噪声引起的听力损失和因氨基糖苷治疗而引起的听力损失。我们还确定了这些分子如何影响内耳毛细胞的下游机制。此外，Notch信号在内耳发育中的新作用。 Notch信号传导不仅指定内耳毛和支撑细胞之间的细胞命运，而且还调节了感觉上皮祖细胞分化的时机并维持这些细胞群体。

项目成果

Hydrogen protects vestibular hair cells from free radicals

• DOI: 10.3109/00016489.2010.486799
• 发表时间: 2010-09
• 期刊: Acta Oto-Laryngologica
• 影响因子: 1.4
• 作者: A. Taura;Y. Kikkawa;T. Nakagawa;J. Ito

Organised haematoma of the sphenoid sinus mimicking a pituitary tumour

• DOI: 10.1017/s002221510900574x
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF LARYNGOLOGY AND OTOLOGY
• 影响因子: 1.7
• 作者: Nakagawa, T.;Kawai, Y.;Ito, J.
• 通讯作者: Ito, J.

臨床家たちが語るDDSの臨床応用

临床医生讨论DDS的临床应用

• 发表时间: 2009
• 期刊: Drug Delivery Syst. 24
• 作者: 松村保広;加藤哲郎;濱口哲弥;南野哲男;坂本達則
• 通讯作者: 坂本達則

Bionic cochlear epithelium : a new concept for totally implantable hearing device. The 11th International Conference on Cochlear Implants and Other Implantable Auditory Technologies

仿生耳蜗上皮：完全植入式听力设备的新概念。

• 发表时间: 2010
• 作者: Nakagawa T;Inaoka T;Shintaku H;Kawano S;Wada H;Kumakawa K;Naito Y;Sakamoto T;Ito J
• 通讯作者: Ito J

Insulin-Like Growth Factor-1 Protects Mouse Cochlear Hair Cells from Aminoglycoside Ex Vivo

胰岛素样生长因子 1 离体保护小鼠耳蜗毛细胞免受氨基糖苷的影响

• 发表时间: 2010
• 作者: Chalermsarp N;Aramaki O;Azuma M;et al.;Yushi Hayashi ; Norio Yamamoto ; Takayuki Nakagawa ; Juichi Ito
• 通讯作者: Yushi Hayashi ; Norio Yamamoto ; Takayuki Nakagawa ; Juichi Ito