Post-sequencing functional studies on the pufferfish (Takifugu rubripes) genome

河豚（Takifugu rubripes）基因组的后测序功能研究

基本信息

批准号：
14104008
负责人：
WATABE Shugo
金额：
$ 72.38万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (S)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2006
项目状态：
已结题

项目摘要

1.Sequencing of 24,398 ESTs generated from 15 different adult and juvenile torafugu tissues resulted in 10,116 gene tags when compared with the genome data. The EST assemblies showed strong conservation with those of zebrafish, although significant differences remained between the two species.2.Muscle specific genes, myosin heavy chain (MYH) and tropomyosin (TPM), and genes encoding myogenic regulatory factors (MyoD family) such as MyoD and myogenin were analyzed in silico and in vivo. Comparison with mammalian counterparts revealed that although the number of the MyoD family genes was almost the same, torafugu had MYHs and TPMs about twice as many as mammalian counterparts, which suggests genome duplication in teleost.3.A total of 28 MYHs comprising 13 fast skeletal, 5 cardiac, 2 slow skeletal, 1 superfast, 2 smooth and 5 nonmuscle, were found in torafugu.4.Torafugu fast skeletal MYHs were distributed across five genomic regions, three of which contained MYH clusters. While human 6 fast skeletal MYHs formed one cluster, its syntenic region in torafugu was duplicated, although each locus contained a single MYH. These results suggest that fast skeletal MYHs evolved independently in teleosts and tetrapods after they had diverged from a common ancestor.5.Bacterial isolates from the torafugu skin were agglutinated by pufflectin, but could not adhere to the torafugu skin.6.pIgR was isolated as an transporter of the skin mucus IgM and, unlike mammals, torafugu pIgR was expressed not only in the intestine but also in the skin. A fragment of pIgR could bind to tetrameric IgM in the skin mucus.7.Marker genes of antigen presenting cells, CD11c, CD40 and MHC class IIα, and genes encoding costimulatory B7 molecules, B7-H1, B7-H3 and B7-H4, isolated from torafugu represent the first of these kinds from teleosts.

1.与基因组数据相比，从15种不同的成年和幼体Torafugu组织产生的24,398个EST产生了10,116个基因标签。 EST组件对斑马鱼的保护表现出强烈的保护，尽管这两个规格之间仍然存在显着差异。2。肌肉特异性基因，肌球蛋白重链（MYH）和topomyosin（TPM）以及编码肌源性调节因子（肌无能家族）的基因，例如在硅质中分析了硅质和肌蛋白。 Comparison with mammalian counterparts revealed that although the number of the MyoD family genes was almost the same, torafugu had MYHs and TPMs about twice as many as mammalian counterparts, which suggests genome duplication in teleost.3.A total of 28 MYHs completing 13 fast skeletal, 5 cardiac, 2 slow skeletal, 1 superfast, 2 smooth and 5 nonmuscle, were found in torafugu.4。torafugu快速骨骼myhs分布在五个基因组区域，其中三个包含myh簇。尽管Human 6快速骨骼MYHS形成了一个集群，但在Torafugu中的同步区域被复制，尽管每个基因座都包含一个MYH。 These results suggest that fast skeletal MYHs evolved independently in teleosts and tetrapods after they had diverged from a common ancestor.5.Bacterial isolates from the torafugu skin were agglutinated by pufflectin, but could not adhere to the torafugu skin.6.pIgR was isolated as an transporter of the skin mucus IgM and, unlike mammals, torafugu pIgR was expressed not only in肠子，但也在皮肤中。 PIGR片段可以与皮肤粘液中的四聚体IgM结合。7。抗原呈现细胞的标记基因，CD11C，CD40和MHCIIα类，以及编码Costimimulation B7分子，B7-H1，B7-H1，B7-H3和B7-H4的基因，从Torafugu中分离为Torafuugu，代表了Torefugu。

项目成果

期刊论文数量（76）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits

DOI：
10.1007/s00251-003-0582-9
发表时间：
2003-07
期刊：
Immunogenetics
影响因子：
3.2
作者：
Y. Yoshiura;I. Kiryu;A. Fujiwara;H. Suetake;Yuzuru Suzuki;T. Nakanishi;M. Ototake
通讯作者：
Y. Yoshiura;I. Kiryu;A. Fujiwara;H. Suetake;Yuzuru Suzuki;T. Nakanishi;M. Ototake

Identification of three isoforms for mitochondrial adenine nucleotide translocator in the pufferfish Takifugu rubripes

DOI：
10.1016/j.mito.2005.01.003
发表时间：
2005-06-01
期刊：
MITOCHONDRION
影响因子：
4.4
作者：
Itoi, S;Misaki, R;Watabe, S
通讯作者：
Watabe, S

"トラフグを中心に魚類体表の生体防御機構をさぐる"海洋生命系のダイナミクス・シリーズ第2巻海洋生物の機能生命は海にどう適応しているか(竹井祥郎編)

《以虎河豚为中心研究鱼类体表的生物防御机制》海洋生物系统动力学系列第2卷海洋生物的功能生命如何适应海洋（武井义郎主编）

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
H.Oguri;et al.;池田大介ら;鈴木譲ら
通讯作者：
鈴木譲ら

Isolation of microsatellite markers by in silico screening implicated for genetic linkage mapping in Japanese pufferfish Takifugu rubripes

通过计算机筛选分离微卫星标记，用于日本河豚红鳍东方鲀的遗传连锁图谱

DOI：
发表时间：
2004
期刊：
Fish.Sci. 70
影响因子：
0
作者：
Y.Nakamura;M.Ando;M.Seoka;K.Kawasaki;Y.Sawada;S.Miyashita;T.Okada;H.Kumai;Y.Tsukamasa.;Megumi Hatori et al.;Furukawa S et al.
通讯作者：
Furukawa S et al.

Cloning, expression, and characterization of fugu CD4, the first ectothermic animal CD4