Studies on Fish Sarcoplasmic Reticulum

鱼类肌质网的研究

基本信息

批准号：
63560198
负责人：
WATABE Shugo
金额：
$ 1.41万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560198/
关键词：
Sarcoplasmic reticulum Plaice Carp Ca^<2+> uptake Rigor mortis Temperature acclimation Storage experiment Ca^<2+> pump カルセクエストリン

项目摘要

Sarcoplasmic reticulum (SR) is an organ which controls muscle intracellular Ca^<2+> concentration and found in fish muscle. This study deals with characterization of SR from a few fishes and its possible role in rigor mortis progress.1. Spiked plaice was stored at 0 or 10ﾟC and examined for changes in Ca^<2+> concentration in the ordinary muscle SR by electron microscopic observation. Ca^<2+> in SR disappeared faster at 0ﾟC than at 10ﾟC with a faster development in rigor tension at 0ﾟC. In addition, fragmented SR prepared from plaice showed the lowest Ca^<2+> uptake when measured at 0ﾟC.2. Plaice myofibrillar Mg^<2+>-ATPase activity was lower at a reaction temperature of 0ﾟC than at 10ﾟC in the absence of Ca^<2+>, the condition corresponding to a relaxed state of muscle. Since the previous results suggest that intracellular Ca^<2+> concentration increases quickly when plaice is stored at 0ﾟC, myofibrillar Mg^<2+>-ATPase activity was measured at this temperature in the presence of Ca^<2+>. The activity was higher than that at 10ﾟC in the absence of Ca^<2+>.3. Fragmented SR from carp ordinary muscle was further purified into heavy and light SR fractions. HSR fraction contained many round vesicles, some of them with feet structures on the surface. SDS-gel electrophoresis revealed that carp SR was composed mainly of Ca^<2+> pump protein with a subunit molecular weight of 95,000 (95kDa) and of 70kDa, 35kDa and 10kDa Ca^<2+>-binding proteins.4. Carp acclimated to 30ﾟC was faster in rigor mortis progress than that acclimated to 10ﾟC when stored at 0ﾟC after spiking. Correspondingly, Ca^<2+> uptake by SR from warm-acclimated carp was lower at a reaction temperature of 0ﾟC than that from cold-acclimated carp.5. The above results suggest that fish SR plays an important role in rigor mortis in terms of affecting intracellular Ca^<2+> concentration after death.

肌质网（SR）是一种控制肌肉内Ca^<2+>浓度并在鱼肌中发现的器官。这项研究涉及来自一些鱼类的SR的表征及其在严格的Mortis进度中的可能作用。1。将尖刺的斑点存储在0或10 c c，并通过电子显微镜观察检查普通肌肉SR中Ca^<2+>的变化。 SR中的Ca^<2+>在0 c c时消失的速度比10°C的速度快，而在0°C处的严格张力的发展速度更快。另外，从平台制备的碎片SR在0 c.2下测量时显示出最低的Ca^<2+>吸收。在不存在Ca^<2+>的情况下，在0℃的反应温度下，在0℃的反应温度下，Plaice肌原纤维Mg^<2+> - ATPase活性较低，该状态对应于肌肉松弛的状态。由于先前的结果表明，当plaice存储在0℃下时，细胞内Ca^<2+>浓度迅速增加，因此在Ca^<2+>存在下，在此温度下测量了肌原纤维Mg^<2+> - ATPase活性。在没有CA^<2+> 3的情况下，该活性高于10°C时的活性。鲤鱼普通肌肉的碎片SR被进一步纯化成沉重的SR级分。 HSR分数包含许多圆形蔬菜，其中一些蔬菜在地面上有脚结构。 SDS-凝胶电泳表明，鲤鱼SR主要由Ca^<2+>泵蛋白组成，亚基分子量为95,000（95KDA）和70KDA，35KDA，35KDA和10KDA Ca^<2 <2+> - 结合蛋白。4。鲤鱼适应30°C的速度比在峰值后储存在0°C时将其更快地适应于10°C。相应地，在0°C的反应温度下，SR从温饮用的鲤鱼中摄入的Ca^<2+>低于冷仪的鲤鱼5。上述结果表明，在影响细胞内Ca^<2+>死后浓度方面，FISH SR在严峻的Mortis中起重要作用。