Production of functional peptides from livestock and fish waste

利用牲畜和鱼类废物生产功能性肽

基本信息

批准号：
13680648
负责人：
MORIMURA Shigeru
金额：
$ 2.3万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

A procedure for the production of functional food from livestock and fish waste such as bone, skin, and sea bream scales containing collagen was developed. Sixteen commercial enzymes were selected to evaluate their effectiveness for degradation of the waste containing collagen. The hydrolysates had a high anti-radical activity (IPOX_<50>, 0.18 and 0.45 mg/ml) and a high potential for decreasing blood pressure (IC_<50>, 0.46 and 0.71 mg/ml). The hydrolysate from sea bream scales, of which IC50 was 0.57 mg/ml was further studied. Using spontaneously hypertensive rats, oral administration of 300 mg of the peptides/(kg of body weight)/d was shown to significantly (p<0.05) decrease blood pressure. Four peptides that demonstrated high angiotensin I converting enzyme (ACE) inhibitory activities were isolated from the hydrolysate of the scales using chromatographic methods using gel filtration chromatography (Sephadex LH-20), anion-exchange chromatography (UNO Q-1), gel filtration chromatography (Superdex Peptide HR 10/30), and reverse-phase chromatography (Sephasil Peptide C18 column). The ACE inhibitory activities of the four peptides isolated by reverse-phase chromatography were 5 to 20 times higher than that of the unpurified hydrolysate. The amino acid sequences of inhibitory peptides ware determined to Gly-Tyr, Val-Tyr, Gly-Phe and Val-Ile-Tyr; furthermore, synthetic Val-Ile-Tyr and Val-Tyr demonstrated high ACE inhibitory activities with IC_<50> values of 7.5 μM and 16 μM, respectively. The C-terminal amino acid of the peptides, that is most important contribution to substrate binding at the ACE active site, were tyrosine and phenylalanine.

开发了一种从牲畜和鱼类废物（如骨骼，皮肤和海洋花朵尺度）生产功能性食品的程序。选择了16种商业酶来评估其含有胶原蛋白废物的降解的有效性。水解物具有较高的抗自由基活性（IPOX_ <50>，0.18和0.45 mg/ml），并且降低血压的潜力很高（IC_ <50>，0.46和0.71 mg/ml）。从海洋花朵尺度上的水解剂，其中IC50为0.57 mg/ml，进一步研究了。使用自发的高血压大鼠，口服300 mg的肽/（体重的kg）/D显示出显着（p <0.05）降低血压。 Four peptides that demonstrated high angiotensin I converting enzyme (ACE) inhibitory activities were isolated from the hydrolysate of the scales using chromatographic methods using gel filtration chromatography (Sephadex LH-20), anion-exchange chromatography (UNO Q-1), gel filtration chromatography (Superdex Peptide HR 10/30), and reverse-phase chromatography (Sephasil肽C18列）。通过反相色谱分离的四种肽的ACE抑制活性比未纯净的水解物高5至20倍。抑制性胡椒器皿的氨基酸序列确定为Gly-tyr，val-tyr，gly-phe和val-ile-tyr；此外，合成的Val-Ile-TYR和Val-TYR表现出高ACE抑制活性，IC_ <50>值分别为7.5μm和16μm。 Petides的C末端氨基酸是对ACE活性位点底物结合的最重要贡献，是酪氨酸和苯丙氨酸。