Study on microbial community in tidal flat sediment sampled from Shirakawa- and Midorikawa-river.

白川河和绿川河滩涂沉积物中微生物群落的研究。

• 批准号: 20510091
• 负责人: MORIMURA Shigeru
• 金额: $ 2.91万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20510091/
• 关键词: 生態環境; 干潟環境; 干潟; 微生物叢; 16SrRNA遺伝子; 機能性遺伝子; 窒素循環; 硫黄循環; クローン解析; 系統樹解析; Alphaproteobacteria綱; Gammaproteobacteria綱; Deltaproteobacteria綱; 硫酸塩還元細菌; 生態環境; 干潟環境; 干潟; 微生物叢; 16SrRNA遺伝子; 機能性遺伝子; 窒素循環; 硫黄循環; クローン解析; 系統樹解析; Alphaproteobacteria綱; Gammaproteobacteria綱; Deltaproteobacteria綱; 硫酸塩還元細菌

Redox-balanced circulation of nitrogen and sulfur will occur in a tidal flat where both aerobic and anaerobic conditions are shown by changing tide level. Bacteria should take important role in the elemental circulation and unbalanced circulation changes environmental condition worse. We analyzed microbial community in tidal flat sampled from Shirakawa- and Midorikawa-river. From the results in clone analysis targeted to 16S rRNA gene, both aerobic and anaerobic bacteria were detected in all samples and Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Chloroflexi, Bacteroidetes and Actinobacteria were dominant. However, Thiobacillus group was dominant by the analysis targeted to functional genes such as aprA and nirS.

氮和硫的氧化还原平衡的循环将发生在潮汐平坦的潮汐和厌氧条件下，通过变化的潮汐水平显示。细菌应在元素循环中发挥重要作用，而循环不平衡会改变环境状况恶化。我们分析了Shirakawa和Midorikawa-River的潮汐平面中的微生物群落。从靶向16S rRNA基因的克隆分析的结果中，在所有样品，αProteobacteria，Gammaproteobacteria，deltaproteobacteria，Chloroflexi，chloroflexi，细菌性甲虫，细菌性酯和静脉细菌中都检​​测到有氧细菌和厌氧菌细菌。然而，硫核酸杆菌组通过针对功能基因（例如APRA和NIRS）的分析来主导。