A new regenerative therapy of periodontal tissue using periostium and molecular chaperones induction

利用骨膜和分子伴侣诱导的牙周组织新再生疗法

• 批准号: 13672187
• 负责人: KAWAGUCHI Hiroyuki
• 金额: $ 1.86万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672187/
• 关键词: molecular chaperones; stress proteins; gingival fibroblast; gingival epithelial cell; periodontal regeneration; periostium

This research has shown clearly that constitutively proteins are inducted at 37 ℃, 3 hours heat treatment, and that Hsp (heat shock proteins) of stress proteins (chaperon molecule) are inducted at 42℃, 3 hours in both a human gingival epithelium cell (HGK) and a human gingival fibroblast (HGF).After acting A.actinomycetemcomitans on HGK, expressions of stress proteins were analyzed by RT-PCR method and the western blotting method. Expression of Hsp27, Hsp60, and Hsp70 was recognized. These expressions were found in cell layer. As for Hsp90, expression was not accepted by heat treatment of 37℃ nor 42℃ at HGK. When Geranylgeranylacetone, inductive agent of chaperon molecule, was made to act on HGK, expression of mRNA of COX-2 and IL-8 was induced. In HGK, even 37℃ and heat treatment of 3 hours expression of mRNA of COX-2 was increased. In the heat treatment to HGF, expression of Hsp104 was not detected at 37℃ or 42℃. On the other hand, expression of Hsp90 increased at 42℃. Expression of Hsp70 and Hsp40 also increased at 42℃ heat treatment similarly.It is necessary to catch the influence of heat treatment in periosteum connective tissue in vivo based on the effect of warm temperature to gingival tissue.

这项研究清楚地表明，在人牙龈上皮细胞中，在37℃、3小时热处理下诱导组成型蛋白，并且在42℃、3小时热处理下诱导应激蛋白（伴侣分子）的Hsp（热休克蛋白）。 HGK)和人牙龈成纤维细胞(HGF)。A.actinomycetemcomitans作用于HGK后，分析应激蛋白的表达通过RT-PCR方法和Western blotting方法，发现Hsp27、Hsp60和Hsp70在细胞层中表达。至于Hsp90，在HGK中37℃和42℃热处理均不表达。当伴侣分子诱导剂香叶基香叶基丙酮作用于HGK时，诱导COX-2和IL-8的mRNA表达。 HGK，即使37℃热处理3小时，COX-2的mRNA表达也增加。在HGF热处理中，在37℃或42℃下未检测到Hsp104的表达。另一方面，Hsp90的表达增加。 42℃热处理时Hsp70和Hsp40的表达也同样增加。因此有必要在体内捕捉热处理对骨膜结缔组织的影响。温度对牙龈组织的影响。

项目成果

Hasegawa N, Kawaguchi H et al.: "Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair"J Periodont Res. 38. 51-56 (2003)

Hasekawa N、Kawaguchi H 等人：“牙骨质修复过程中 Malassez 上皮细胞残骸的免疫组织化学特征”J periodont Res。

Uchida U, Shiba H et al.: "Expression of IL-1 and IL-8 by human gingival epithelial cells in response to Actinbacillus actinomycetemcomitans"Cytokine. 14. 152-161 (2001)

Uchida U、Shiba H 等人：“人类牙龈上皮细胞响应放线放线杆菌的 IL-1 和 IL-8 表达”细胞因子。

Sakata M, Shiba H, Kawaguchi H et al.: "Osteoprotegerin levels increased by interleukin-1beta in human periodontal ligament cells are suppressed through prostaglandin E2 synthesized de novo"Cytokine. 18(3). 133-139 (2002)

Sakata M、Shiba H、Kawaguchi H 等人：“人牙周膜细胞中白介素-1β 增加的骨保护素水平通过从头合成的前列腺素 E2 受到抑制”细胞因子。

Hasegawa N, Kawaguchi H et.al.: "Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair"J Periodont Res. 38. 51-56 (2003)

Hasekawa N、Kawaguchi H 等人：“牙骨质修复过程中 Malassez 上皮细胞残骸的免疫组织化学特征”J periodont Res。

Fujita T, Shiba H et al.: "Effects of transforming growth gactor-b1 and fibronectin on SPARC expression in cultures of human periodontal ligament cells"Cell Biol. Int. 26(12). 1065-1072 (2002)

Fujita T、Shiba H 等人：“转化生长因子-b1 和纤连蛋白对人牙周膜细胞培养物中 SPARC 表达的影响”Cell Biol。