Pathologic Studies on Induction of Craniomandibular Joint Arthritis is using in Rabbits Escherichia Coli Antigens

大肠杆菌抗原诱导兔颅颌关节关节炎的病理学研究

基本信息

批准号：
13672132
负责人：
KURITA Kenichi
金额：
$ 1.41万
依托单位：
SCHOOL OF DENTISTRY, AICHI-GAKUIN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672132/
关键词：
Arthritis in rabbit enterobacterial antigens Eschericia Coli : 014 抗原誘導関節炎 rabbit cranio waudibular joint enterobacterial anfigens Escherichia coli 0:14 Experimental arthritis

项目摘要

Material and Method : While Japanese male rabbits were sensitized by Escherichia coli 0 : 14 and Freunds incomplete adjuvant. For the control group, rabbits were perfused with physiolosical saline and them purifyied water. On the other hand, for the experimental group, their lower cavity of the left temporomandibular joint (TMJ) were perfused with physiolosical saline and then infused with Escherichia coli 0 : 14 together with purifyied water. Several pieces of the latter were sacrificed on the 7^<th>, 14^<th>, 21st, 28^<th>, 35^<th>, and 56^<th> day after the above procedure for pathological observation by resecling the joint. Based on stainblocker's, Larsen's, Arthur's and Amaji's dassification, we invented a new classification method of our own for giving them comparison using scores.Results : (Control group) : No pathological variation was observed at the basic structure, although the carfilage layer got thinner with time. Score was class 0 throughout the entire period. (Experimental group) : 7^<th> day : An acute exudative inflammation was observed. 14^<th> day : The carfilage was destroyed owing to multiplication of pannus. 21st day : The lesion developed to reach the home, which indicates the transition to chronic inflammation. 28^<th> day : Further lesion development. Typical rheumatiod arthritis was observed, macrophage, plasma cell, conflent follicle of limphocyte, or formation of pannus. After this, it look a turn for the better to get cured gradually. Now we are following up about MMP-3 and obteopontin.As to the scores, arthritis destruction was promoted as described above, and it showed maximum point on the day of 28^<th>, which was (3.00+/-0.00).Remark : As the pathological process of the experimental group is just common to that of human TMJ, it is confimied that this experiment serves not only for illuminating the actual shape of rhemaloid arthritis and also the future therapeulic research.

材料和方法：虽然大肠杆菌0：14和弗朗德斯不完整的佐剂敏感了日本雄性兔子。对于对照组，兔子灌注生理盐水和净化水。另一方面，对于实验组，它们的左颞下颌关节（TMJ）的下部腔被灌注生理盐水，然后与大肠杆菌0：14一起注入纯净水。在7^<th>，14^<th>，21t，28^<th>，35^<th>和56^<th>上以上方法进行病理学观察程序之后的第21、28^<th>和56^<th>重新定位关节。基于Stainblocker's，Larsen's，Arthur's和Amaji的大声疾呼，我们发明了一种新的分类方法，用于使用分数进行比较。恢复：（对照组）：尽管在基本结构上没有观察到病理变化，尽管Carfilage层较薄，但随着时间的流逝。整个期间的分数为0。（实验组）：7^<th>日：观察到急性渗出性炎症。 14^<th>天：由于pannus的繁殖，碳紫红色被摧毁。第21天：病变发展为到达家中，这表明过渡到慢性炎症。 28^<th>日：进一步的病变发育。观察到典型的风湿性关节炎，巨噬细胞，浆细胞，膜状细胞的融合卵泡或Pannus的形成。之后，看起来更好，可以逐渐治愈。现在，我们正在关注MMP-3和Obteopontin。按照上述分数，促进了关节炎的破坏，并且在28^<th>的当天显示了最大点，即（3.00 +/- 0.00）。注释：由于实验组的病理过程只是人类TMJ的常见，因此该实验不仅用于阐明类风湿关节炎的实际形状以及未来的治疗研究。