Cell Death and Cell Proliferation of Myoepithelial cells in Duct-Ligated Submandibular Glands

导管结扎颌下腺肌上皮细胞的细胞死亡和细胞增殖

• 批准号: 13671961
• 负责人: TAKAHASHI Shigeru
• 金额: $ 1.66万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671961/
• 关键词: Submandibular Gland; Myoepithelial Cell; Apoptosis; Cell Proliferation

This study was designed to determine whether apoptosis and proliferation of myoepithelial cells occur in atrophic rat submandibular glands. The excretory duct of the right submandibular gland was doubly ligated with the metal clips. The atrophic right submandibular glands removed after 1-28 days of duct-ligation were investigated using immunohistochemical double staining for actin as a marker for myoepithelial cells and proliferating cell nuclear antigen (PCNA), double staining for actin immunohistochemistry, nick-end-labeling (TUNEL) as a marker for apoptotic cells, and transmission electron microscopy (TEM). A few PCNA- and no TUNEL-positive myoepithelial cells were found in the control submandibular glands taken from animals with no operation. In the experimental glands, PCNA-positive myoepithelial cells were common 2 and 3 days after duct ligation and then decreased in number. TUNEL-positive myoepithelial cells appeared at 2 days and were also identified by TEM. These observations suggest that both apoptosis and proliferation of myoepithelial cells occur, especially in the early phase of atrophy, in the rat submandibular gland.

本研究旨在确定萎缩的大鼠颌下腺是否发生肌上皮细胞凋亡和增殖。将右侧颌下腺的排泄管用金属夹双重结扎。使用肌动蛋白免疫组织化学双染作为肌上皮细胞和增殖细胞核抗原（PCNA）的标记、肌动蛋白免疫组织化学双染、缺口末端标记（ TUNEL）作为凋亡细胞的标记，以及透射电子显微镜（TEM）。在取自未进行手术的动物的对照下颌下腺中，发现了一些 PCNA 阳性肌上皮细胞，没有发现 TUNEL 阳性肌上皮细胞。在实验腺体中，导管结扎后2天和3天PCNA阳性肌上皮细胞常见，然后数量减少。 TUNEL 阳性肌上皮细胞在第 2 天出现，也通过 TEM 进行了鉴定。这些观察结果表明，在大鼠颌下腺中，肌上皮细胞发生凋亡和增殖，尤其是在萎缩的早期阶段。

项目成果

S. Takahashi: "Apoptosis and proliferation of myoepithelial cells in atrophic rat submandibular glands"J. Histochem. Cytochem.. 49(12). 1557-1563 (2001)

S. Takahashi：“萎缩性大鼠颌下腺肌上皮细胞的凋亡和增殖”J。

S.Takahashi: "Proliferation and distribution of myoepithelial cells during atrophy of the rat sublingual gland"J.Oral Pathol.Med.. 32(印刷中). (2003)

S. Takahashi：“大鼠舌下腺萎缩期间肌上皮细胞的增殖和分布”J. Oral Pathol. 32（印刷中）。

S. TAKAHASHI: "Proliferation and Distribution of Myoepithelial Cells During Atrophy of the Rat Sublingual Gland"J. Oral Pathol. Med.. 32, in press. (2003)

S. TAKAHASHI：“大鼠舌下腺萎缩期间肌上皮细胞的增殖和分布”J。

S.Takahashi: "The roles of apoptosis and mitosis in atrophy of the sublingual gland of rat"Tissue Cell. 34(5). 297-304 (2002)

S.Takahashi：“细胞凋亡和有丝分裂在大鼠舌下腺萎缩中的作用”组织细胞。

S. TAKAHASHI: "The Roles of Apoptosis and Mitosis in Atrophy of the Sublingual Gland"Tissue Cell. 34(5). 297-304 (2002)

S. TAKAHASHI：“舌下腺萎缩中细胞凋亡和有丝分裂的作用”组织细胞。