The Roles of Cell Proliferation and Cell Death in Regeneration of Salivary Glands

细胞增殖和细胞死亡在唾液腺再生中的作用

基本信息

批准号：
15591990
负责人：
TAKAHASHI Shigeru
金额：
$ 2.24万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591990/
关键词：
Salivary Gland Regeneration Cell Proliferation Apoptosis 唾液線

项目摘要

【Introduction】The present research aimed to clarify the proliferation and apoptosis of parenchymal cells such as acinar, duct, myoepithelial cells during regeneration of rat salivary glands following atrophy induced by duct ligation.【Methods】Atrophy of the right submandibular gland was induced by excretory duct ligation near its hilum with metal clips, which were removed one week (day 0) after ligation. The right submandibular glands were collected from 0 to day 14 after removal of clips and investigated with PCNA as a marker of proliferating cells, TUNEL as a marker of apoptotic cells, actin-PCNA double immunohistochemistry as a marker of proliferating myoepithelial cells, and transmission electron microscopy.【Results】After one week of ligation, there were many remaining ducts and a few acini in the atrophic glands. At day 3 after discontinuing the ligation, newly formed acini appeared and thereafter increased in number and maturity. many residual and newly formed acinar cells showed positive reaction to PCNA especially at day 4 and 5. The PCNA-positive duct cells decreased in number with the regeneration. Double-positive cells, PCNA-positive myoepithelial cells were also observed, especially between days 2 and 4. A few TUNEL-positive acinar and duct cells were identified during regeneration. Some mitosis and a few apoptosis of acinar and duct cells were also identified by transmission electron microscopy. In addition, a mitotic figure of myoepithelial cell was first identified ultrastructurally.【Conclusions】During regeneration of the submandibular gland after atrophy, both residual and newly formed acinar cells proliferate actively. There is also apoptosis of duct and acinar cells, which participates in remodeling the structure of salivary gland tissue after parenchymal cell proliferation. In addition, it is confirmed that myoepithelial cells are able to proliferate and participate in the regeneration of salivary glands.

【简介】本研究旨在阐明腺泡、导管、肌上皮细胞等实质细胞在导管结扎致萎缩后再生过程中的增殖和凋亡情况。【方法】排泄诱导右侧颌下腺萎缩。用金属夹在其肺门附近结扎导管，结扎后一周（第 0 天）将其移除。取夹子后第0天至第14天收集颌下腺，用PCNA作为增殖细胞的标记，TUNEL作为凋亡细胞的标记，肌动蛋白-PCNA双免疫组化作为增殖肌上皮细胞的标记，并使用透射电镜进行研究。【结果】结扎1周后，萎缩处仍有较多导管残存和少量腺泡。停止结扎后第3天，新形成的腺泡出现，其后数量和成熟度均增加，许多残留的和新形成的腺泡细胞对PCNA表现出阳性反应，尤其是在第4天和第5天。PCNA阳性导管细胞数量减少。随着再生，还观察到了双阳性细胞、PCNA 阳性肌上皮细胞，尤其是在第 2 天和第 4 天之间。在此期间鉴定出了一些 TUNEL 阳性腺泡和导管细胞。透射电镜观察发现腺泡和导管细胞有一定的有丝分裂和少量凋亡，超微结构上首次发现了肌上皮细胞的有丝分裂。新形成的腺泡细胞增殖活跃，导管和腺泡细胞也发生凋亡，参与唾液腺组织结构的重塑。此外，已证实肌上皮细胞能够增殖并参与唾液腺的再生。