The study of an effect of Porphyromonas gingivalis heat shock protein on bone resorption

牙龈卟啉单胞菌热休克蛋白对骨吸收影响的研究

基本信息

批准号：
13671911
负责人：
AMANO Shigeru
金额：
$ 1.86万
依托单位：
Meikai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671911/
关键词：
P. gingivalis GroEL bone resorption γδT cells RANKL P. gingivalis groEL γδ T細胞 Porphyromonas gingivails HSP bone resorption T cell ODF

项目摘要

Porphyromonas gingivalis (P. gingivalis) is a Gram-negative bacterium implicated in the pathology of adult periodontitis, an involving destruction of alveolar bone. Chaperonins (cpns) are intracellular oligomeric protein complexes that fold and refold proteins in a catalytic manner. Cpns are also major immunogen in infectious diseases, because of some evidences that Escherichia coli GroEL and Mycobacterial cpn 60s can induce cytokine synthesis, and stimulate cytokine-dependent bone resorption. We have shown mechanisms of bone resorption by components of P. gingivalis such as lipopolysaccharide or fimbrillin by using 14-day-old mouse embryonic calvarial bone cell culture system. However, little is known concerning P. gingivalis chaperonin (Pcpn) 60 (GroEL) on bone resorption. In the present study, we show that P. gingivalis GroEL stimulates bone resorption in the presence of γδT cells was inhibited by osteoprotegerin (OPG). The osteoclast formation by stimulation of P. gingivalis GroEL was observed by coculture between precursor cells (Mac-1(+), c-Fms(+), RANK(+)) treated with both M-CSF (10ng/ml) and suboptimal dose of RANKL, and γδT cells, but not αβT cells. These data suggest that if GroEL was released from P. gingivalis and γδT cells was increased in alveolar bone or periodontal tissue, it could induce osteoclast formation.

牙龈卟啉单胞菌 (P. gingivalis) 是一种革兰氏阴性细菌，与成人牙周炎的病理学有关，涉及牙槽骨的破坏。伴侣蛋白 (cpns) 是细胞内寡聚蛋白复合物，以催化方式折叠和重新折叠蛋白质。感染性疾病中的主要免疫原，因为一些证据表明大肠杆菌 GroEL 和分枝杆菌 cpn 60s 可以诱导细胞因子合成，并刺激细胞因子依赖性骨吸收。我们通过使用 14 日龄小鼠胚胎颅骨骨细胞培养系统，显示了牙龈卟啉单胞菌成分（如脂多糖或 fimbrillin）的骨吸收机制。已知牙龈卟啉单胞菌伴侣蛋白 (Pcpn) 60 (GroEL) 对骨吸收的影响。牙龈卟啉单胞菌 GroEL 在 γδT 细胞存在的情况下刺激骨吸收，并被骨保护素 (OPG) 抑制通过前体细胞 (Mac-1(+)、c-Fms(+) 之间的共培养，观察到刺激牙龈卟啉单胞菌 GroEL 形成的破骨细胞。 , RANK(+)) 用 M-CSF (10ng/ml) 和次优剂量的 RANKL 以及 γδT 细胞治疗，但不是 αβT这些数据表明，如果牙龈卟啉单胞菌释放GroEL并且牙槽骨或牙周组织中的γδT细胞增加，则可以诱导破骨细胞形成。