Identification of neural stem cells in neural retina, ciliary body and iris

神经视网膜、睫状体和虹膜神经干细胞的鉴定

• 批准号: 13671834
• 负责人: TAKAHASHI Masayo
• 金额: $ 2.3万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671834/
• 关键词: neural stem cells; neural progenitor cells; retinal transplantation; embryonic retina; ciliary body; iris; homeobox gene; photoreceptor cells; 胎仔網膜; 毛様体上皮; 虹彩上皮; Crx

Embryonic retina is one of the possible cell sources that will repair degenerated retina such as retimtis pigmentosa. Retinal progenitor cells isolated from embryonic rats could be cultured and expanded in serum free medium with both epidermal growth factor and basic fibroblast growth factor. We analyzed the properties of two different retinal progenitor cells in terms of culture periods. Retinal progenitor cells from embryonic retina could be expanded keeping immature cell properties and had the ability to migrate into degenerated adult retina from subretinal space after transplantation. They differentiated into neurons and glias, even into photoreceptor cells both in vitro and in vivo. However, they appeared to lose their tissue specificity after a long-term cultureWe also showed that iris and ciliary tissue in the adult rat eye, which are embryonically related to the neural retina, can generate cells expressing differentiated neuronal antigens. In addition, the Crx gene transfer induced the specific antigens for rod photoreceptors in the iris-derived cells, which was not seen in the adult hippocampus-derived neural stem cells. Our findings demonstrate a remarkable plasticity of adult iris tissue with potential clinical applications, as autologous iris tissue can be feasibly obtained with peripheral iridectomy

胚胎视网膜是修复退化视网膜（例如色素性视网膜）的可能细胞来源之一。从胚胎大鼠中分离的视网膜祖细胞可以在含有表皮生长因子和碱性成纤维细胞生长因子的无血清培养基中培养和扩增。我们分析了两种不同视网膜祖细胞在培养周期方面的特性。来自胚胎视网膜的视网膜祖细胞可以在保持未成熟细胞特性的情况下进行扩增，并且能够在移植后从视网膜下间隙迁移到退化的成人视网膜中。它们在体外和体内分化为神经元和神经胶质细胞，甚至分化为感光细胞。然而，经过长期培养后，它们似乎失去了组织特异性。我们还表明，成年大鼠眼中的虹膜和睫状组织在胚胎上与神经视网膜相关，可以产生表达分化神经元抗原的细胞。此外，Crx基因转移在虹膜来源的细胞中诱导了视杆光感受器的特异性抗原，这在成人海马来源的神经干细胞中未见。我们的研究结果表明，成人虹膜组织具有显着的可塑性，具有潜在的临床应用价值，因为自体虹膜组织可以通过周边虹膜切除术获得

项目成果

Harata M. et al.: "Induction of photoreceptor-specific phenotypes in adult mammalia iris tissue"Nature Neuroscdeace. 4(12). 1163-1164 (2001)

Harata M.等人：“成年哺乳动物虹膜组织中光感受器特异性表型的诱导”Nature Neuroscdeace。

Akita J, et al.: "Neuronal differentiation of adult rat hippocampus-derived neural stem cell transplanted into embryonic rat explanted retinas with retinoic acid pretreatment"BRAIN RESEARCH. 954. 286-293 (2002)

Akita J 等人：“用视黄酸预处理将成年大鼠海马源性神经干细胞移植到胚胎大鼠移植视网膜中的神经分化”BRAIN RESEARCH。

Akagi T.et al.: "Different characteristics of rat retinal progenitor cells from different culture periods"Neuroscience Letter. 341(3). 213-216 (2003)

Akagi T.等人：“来自不同培养时期的大鼠视网膜祖细胞的不同特征”《神经科学快报》。

Kawasaki H, et al.: "Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity"Proc Nati Acad Sci USA. 99. 1580-1585 (2002)

Kawasaki H 等人：“通过基质细胞衍生的诱导活性从灵长类 ES 细胞中生成多巴胺能神经元和色素上皮”Proc Nati Acad Sci USA。

Kurimoto Y. et al.: "Transplantation of adult rat hippocampus-derived neural stem cells into retina injured by transient ischemia"Neurosci Lett. 306. 57-60 (2001)

Kurimoto Y. 等人：“将成年大鼠海马来源的神经干细胞移植到短暂性缺血损伤的视网膜中”Neurosci Lett。