The relationship between the RPE cell function and its expression of growth factor receptors.

RPE细胞功能与其生长因子受体表达之间的关系。

基本信息

批准号：
04454441
负责人：
TAKAHASHI Masayo
金额：
$ 4.16万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

Retinal pigment epitheliaum (RPE) is a monolayr of the cells existing between neural retina and choroid. RPE cells are known to play an important role in proliferative vitreoretinopathy (PVR) . In PVR abnormal proliferation of RPE cells is observed. This abnormal proliferation may be caused by a lot of environmental changes of RPE cells. Releasing of many kind of growth factors into the vitreous cavity following to the breakdown of blood-ocular barrier may be one of the most important reasons. Following four themes are studied. 1) To study what kind of growth factors and their receptors are expressed in cultured RPE cells--All planned screening were carried out and Transforming growth factor beta, platelet derived growth factors and their receptors, acidic and basic fibroblast growth factors and their receptors, interleukin-1 and their receptors, tumor necrosis factor, insulin like growth factors, etc. were found to be expressed in cultured RPE cells. 2) To investigate if it is possible to transfect the growth factor gene into cultured RPE cells or not. --Transient expression of transfected gene in primary cultured RPE was successful, but permanent expression was difficult. It is now in progress using immortalized RPE cell line cells. 3) To evaluate how the function of RPE cells change when various growth factor receptor gene expression is increased or decreased. --We are waiting for the results of project 2) 4) Looking for a novel growth factor receptor specific for RPE cells. --PCR was carried out using primers of conserved sequences between several FGF receptors to obtain RPE specific clones of FGF receptors. We've got a couple of clones. The DNA sequence and the function of them are now being analyzed.

视网膜色素上皮（RPE）是存在于神经视网膜和脉络膜之间的单层细胞。已知RPE细胞在增殖性玻璃体视网膜病变(PVR)中发挥重要作用。在 PVR 中观察到 RPE 细胞的异常增殖。这种异常增殖可能是由RPE细胞的大量环境变化引起的。血眼屏障破坏后多种生长因子释放到玻璃体腔内可能是最重要的原因之一。研究了以下四个主题。 1) 研究培养的RPE细胞表达什么样的生长因子及其受体--进行了所有计划的筛选，转化生长因子β、血小板源性生长因子及其受体、酸性和碱性成纤维细胞生长因子及其受体，培养的RPE细胞中发现白细胞介素1及其受体、肿瘤坏死因子、胰岛素样生长因子等表达。 2) 探讨是否可以将生长因子基因转染到培养的RPE细胞中。 --转染基因在原代培养的RPE中瞬时表达成功，但永久表达困难。目前正在使用永生化 RPE 细胞系细胞进行研究。 3) 评价当各种生长因子受体基因表达增加或减少时，RPE细胞的功能如何变化。 --我们正在等待项目2) 4) 寻找针对RPE细胞的新型生长因子受体的结果。 --使用几种FGF受体之间保守序列的引物进行PCR以获得FGF受体的RPE特异性克隆。我们有几个克隆人。目前正在分析 DNA 序列及其功能。