Effects or hypergravity on genes expressed in the rat inner car

超重力对大鼠内车基因表达的影响

基本信息

批准号：
13671778
负责人：
DOI Katsumi
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Recovery from balance disorders aster spaceflight is supposed.to reflect the readaptation to normal gravitational environment (1G). from microgravity, In the present study, to elucidate a role of otoconia in adaptation to altered gravity, we examined the effects of hypergravity (2G) on nor polo and synthesis of otoconia in young adult rats, Neither otoconial morphology nor otoconial synthesis was affected by up to 1 week exposure to hypergravity, These results suggest that changes in neurotransmission at the synapses or flit peripheral and/or contral vestibular system rather than the Changes in otoconial morphology and synthesis may be involved in adaptive mechanismsWe also assessed the changes in mRNA expression of glutamate receptors in the vestibular system of rats exposed to hypergravily for 2 h to 1 week using real-time quantitative RT-PCR, Tho expression of GluR2 and NRI in khe uvula/nodulus and NR1 in the medial vestibuiar nucleus increased after 2 h of hypergravity, The expresion of GluR2 in vestibular ganglion cells decreased after 1 week of hypergravity ; The results suggested that the animals adapted to the hypergravity by enhancing the crrebellar inhibition of the vestibular nucleus via an activation of the NR1 and GluR2 on tha Purkinje cells in uvula/nodules especially of the early phase following hypergravity, while in the later phase by reducing the neurotransmission between the vestibular hair cells and the primary vestibular neurons via down-regulation of the postsynaptic GluR2 in the vestibular periphery

假设从平衡疾病中恢复Aster SpaceFlight。为了反映正常重力环境的读数（1G）。从微作用，在本研究中，到阐明耳鸣症在适应重力改变中的作用，我们检查了超级强度（2G）对年轻大鼠的polo（2G）对耳朵的影响，在年轻人大鼠中的合成，在1周的曝光中，在1周的曝光中都没有影响到型号的曝光，这些结果均未影响这些型号，这些结果是这些型号的指示性，这些结果是这些指示性的指示性，这些结果是这些指示性的指示性，这些结果是这些指示性的指示性，这些结果是这些指示性的指示性，这些结果是这些指示性的指示性。或可能参与自适应机制的外周和/或对侧前庭系统，而不是涉及耳鼻核形态和合成的变化，还评估了在高度杂质的大鼠前庭中谷氨酸受体的mRNA表达的变化，使用超级杂质的大鼠系统使用2小时至1周，使用实时定量rt rt ul2和nr 2和NR 2和nr uv uv inr uv inr uv uv uv inr uv inr uv inr uv inr uv inr 2超级重度2小时后，内侧前庭核中的NR1增加，前庭神经节细胞中GLUR2的表达减少了1周后降低。 The results suggested that the animals adapted to the hypergravity by enhancing the crrebellar inhibition of the vestibular nucleus via an activation of the NR1 and GluR2 on tha Purkinje cells in uvula/nodules especially of the early phase following hypergravity, while in the later phase by reducing the neurotransmission between the vestibular hair cells and the primary vestibular neurons via down-regulation of the前庭外围的突触后glur2