DNA microarray analysis on frequency tuning and tonotopical organization in the mouse cochlea

DNA 微阵列分析小鼠耳蜗频率调谐和音调组织

基本信息

批准号：
15591811
负责人：
DOI Katsumi
金额：
$ 2.18万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591811/
关键词：
Cochlea DNA microarray analysis apical turn basal turn real time PCR frequency tunig 周波数選択制回転別 TOTAL RNA

项目摘要

To understand the mechanism of frequency tuning and tonotopical organization in the mouse cochlea, we characterized mRNA expression in the apical and basal parts of the mouse cochlea with a DNA microarray analysis.DNA microarray analysis revealed that the mRNA expression of 184 out of 19801 genes on the DNA chip was more than three times up-regulated in the apical part of the mouse cochlea and that the mRNA expression of 181 out of 19801 genes on the DNA chip was more than three times up-regulated in the basal part. Further study by using a real-time PCR technique confirmed that the expressions of Slc4al (Cl-HC03 exchanger) was up-regulated by 3.12 times in the apical part and that the expressions of six genes including Gabt1 (GABA receptor β1), Nstr2 (Neurotensin receptor), and Tac1 (Substance P) were up-regulated more than three times in the basal part.DNA microarray analysis focusing on the efferent neurotransmission via acetylcholine receptors (AChR) and GABA receptors (GABAR) demonstrated that the mRNA expression of AChR subunits was almost comparable in the apical and basal parts of the mouse cochlea while the mRNA expression of GABAR subunits was not always even in both parts. The mRNA expressions of subunits γ3,θ,δ, and ρ were almost equal in both parts. However, the expressions of β1,β2,γ1, and γ2 were more than two times up-regulated in the basal part of the mouse cochlea.These results indicate that the location/place-specific mRNA expression in hair cells and spiral ganglion cells in the mouse cochlea might determine the frequency tuning and tonotopical organization.

为了了解小鼠耳蜗中的频率调整和调节性组织的机制，我们通过DNA微阵列分析表征了小鼠耳蜗的顶端和基本部分中的mRNA表达。DNA微阵列分析表明，在DNA切碎中，在19801年基因中，184的mRNA表达在DNA切碎中的表达超过了180次，而MRNA在180年中的表达超出了180次的MRNA，而MRNA则是MRNA的180次，而MRNA的MRNA表达则超出了MRNA的180次和鼠标的一部分。在基本部分，DNA芯片上的基因上调了三倍以上。 Further study by using a real-time PCR technique confirmed that the expressions of Slc4al (Cl-HC03 exchanger) was up-regulated by 3.12 times in the apical part and that the expressions of six genes including Gabt1 (GABA receptor β1), Nstr2 (Neurotensin receptor), and Tac1 (Substance P) were up-regulated more than three times in the basic part.DNA microarray analysis focusing on通过乙酰胆碱受体（ACHR）和GABA受体（GABAR）的有效神经传递表明，ACHR亚基的mRNA表达在小鼠耳蜗的根尖和基本部分中几乎是可比的，而Gabar亚基的mRNA表达并不总是在两个部分中。但是，在小鼠耳蜗的基本部分中，β1，β2，γ1和γ2的表达超过两次。这些结果表明，小鼠耳蜗中毛细胞和螺旋神经节细胞中的位置/位置特异性mRNA表达可能决定了频率调整和调节性组织。