Experimental Study on skeletal Tissue allotransplant

同种异体骨骼组织移植的实验研究

• 批准号: 13671514
• 负责人: MURAMATSU Keiichi
• 金额: $ 2.62万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671514/
• 关键词: Limb allograft; immunotolerance; chimerism; H-Y Antigen; PCR; rat; skeletal tissue; FK506; Microchimerism

Chimerism following allogeneic organ transplantation may be a mechanism for induction of donor-specific graft acceptance. However, little is known about graft cell migration following skeletal tissue allograft. Our study investigated a cell exchange phenomenon following whole-limb allotransplantation. Experimental animals were DA rats and Lewis rats and right hind-limbs were orthotopically transplanted in sex-mismatched pairs. FK506 (Fujisawa Pharmaceutical Co., Osaka, Japan) was administered after transplantation for immunosuppression. Chimerism was assessed by semi-quantitative polymerase chain reaction for the Y-chromosome. Our results indicated that 1) Y-chromosome didn't behave as male specific minor antigen in whole limb allograft, although it worked as antigen in skin grafts 2) function of transplanted limb was roughly recovered 70% compared to normal side, 3) donor cells survived in the graft through 1 year after transplantation and gradually migrated into recipient's lymphoid tissue and bones, 4) donor cells in the grafted limb (involving bone, muscle and skin tissues) were gradually replaced by recipient's cells. Our study demonstrated stable and high level chimerism following limb allotransplantation.

同种异体器官移植后的嵌合可能是诱导供体特异性移植物接受的机制。然而，对于骨骼组织同种异体移植后的移植细胞迁移知之甚少。我们的研究调查了全肢同种异体移植后的细胞交换现象。实验动物为DA大鼠和Lewis大鼠，右后肢按性别不匹配的配对进行原位移植。移植后给予FK506（Fujisawa Pharmaceutical Co, Osaka, Japan）用于免疫抑制。通过 Y 染色体的半定量聚合酶链式反应评估嵌合状态。我们的结果表明 1) Y 染色体在整个肢体同种异体移植物中并不充当男性特异性次要抗原，尽管它在皮肤移植物中充当抗原 2) 与正常侧相比，移植肢体的功能大致恢复了 70%，3) 供体移植后细胞在移植物中存活1年，并逐渐迁移到受体的淋巴组织和骨骼中，4）移植肢体中的供体细胞（包括骨骼、肌肉和皮肤组织）逐渐被受体的细胞所取代细胞。我们的研究表明肢体移植后具有稳定且高水平的嵌合状态。

项目成果

Muramatsu K., et al.: "Behavior of Male-Specific Minor Histocompatibility Antigen in Skin and Limb Transplantation"J. Surg. Res. Accepted. 2003.

Muramatsu K. 等人：“皮肤和肢体移植中男性特异性次要组织相容性抗原的行为”J。

Muramatsu K., et al.: "Fate of donor cells in vascularized bone grafts: identification of systemic chimerism by the polymerase chain reaction"Plast Reconstr Surg.. 111(2). 763-772 (2003)

Muramatsu K. 等人：“血管化骨移植物中供体细胞的命运：通过聚合酶链式反应鉴定全身嵌合现象”Plast Reconstr Surg.. 111(2)。

村松慶一: "血管柄付き同種骨移植の細胞置換過程"今日の移植. 15・1. 71-85 (2002)

Keiichi Muramatsu：“血管化骨同种异体移植的细胞替代过程”《今日移植》15・1（2002）。

Muramatsu K.: "Behavior of male-specific antigen in limb transplantations"Journal of Surgical Research. (accepted). (2003)

Muramatsu K.：“肢体移植中男性特异性抗原的行为”外科研究杂志。