Experimental Study on Skeletal Tissue Allotransplant

同种异体骨骼组织移植的实验研究

• 批准号: 15591578
• 负责人: MURAMATSU Keiichi
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591578/
• 关键词: limb Allograft; immunotolerance; chimerism; PCR; rat; トランスゲニックラット; 運動器

Although cell traffic from the graft into the recipient and from the recipient into the graft had been noticed in allogeneic organ transplantation, little is known following whole-limb allografting. Because vascularized limb grafts have numerous undifferentiated stem cells in the bone marrow, donor cell migration into the recipient may be more frequent as compared to other organ transplants. This study was conducted to define cell migration between donor and recipient by use of polymerase chain reaction.Sixty-seven vascularized hind limb allotransplantations were performed in rat sex-mismatched pairs across minor and major histocompatibility barriers. The recipient animals were treated with FK506 immunosuppression and assessed for a period of 48 weeks post-transplantation. The ratio of donor and recipient cells was evaluated by semi-quantitative PCR using the specific primers of the Y-chromosome.Allografted limbs had no rejection episode until the final assessment. The male recipient cells were detected in female grafts not at 1 week but at 48 weeks after transplantation. The ratio of recipient cells was more than 10% in the femur, tibia and 1 to more than 10% in the gastrocnemius muscle, leg skin and plantar skin. The male donor cells were detected in the humerus and tibia in the female recipient and the ratio of donor cells was 1% to 10% at 48 weeks. No donor cells were detected in the gastrocnemius muscle and leg skin in recipient.Our results demonstrated that recipient derived cells gradually migrated into the grafted bone, muscle and skin cells with the duration of time. Donor derived cells migrated into the healthy bones (two-way cell traffic) but not into the healthy muscle and skin (one-way cell traffic). Because active regeneration occurs in the grafted limb to compensate graft damage secondary to ischemia and operative intervention, recipient-derived stem cells may mediate a muscular and dermo-epidermal renewal.

尽管在同种异体器官移植中已经注意到从移植物到受体以及从受体到移植物的细胞运输，但对于全肢同种异体移植却知之甚少。由于血管化肢体移植物的骨髓中含有大量未分化的干细胞，因此与其他器官移植相比，供体细胞迁移到受体中可能更频繁。这项研究的目的是通过聚合酶链反应来定义供体和受体之间的细胞迁移。在性别不匹配的大鼠中进行了 67 例血管化后肢同种异体移植，跨越了次要和主要的组织相容性障碍。受体动物接受 FK506 免疫抑制治疗，并在移植后 48 周内进行评估。使用Y染色体特异性引物通过半定量PCR评估供体和受体细胞的比例。同种异体移植肢体在最终评估之前没有出现排斥反应。雄性受体细胞不是在移植后 1 周而是在移植后 48 周时在雌性移植物中检测到的。股骨、胫骨中受体细胞比例大于10%，腓肠肌、腿部皮肤和足底皮肤中受体细胞比例为1%至10%以上。 48周时，在女性受者的肱骨和胫骨中检测到男性供体细胞，供体细胞的比例为1%至10%。在受者的腓肠肌和腿部皮肤中未检测到供体细胞。我们的结果表明，受者来源的细胞随着时间的推移逐渐迁移到移植的骨、肌肉和皮肤细胞中。供体来源的细胞迁移到健康的骨骼（双向细胞交通），但不迁移到健康的肌肉和皮肤（单向细胞交通）。由于移植肢体会发生主动再生，以补偿继发于缺血和手术干预的移植物损伤，因此受体来源的干细胞可以介导肌肉和真皮表皮的更新。

项目成果

Muramatsu K: "Behavior of male-specific antigen in limb transplantation"Journal of surgical research. 115(1). 106-12 (2003)

Muramatsu K：“男性特异性抗原在肢体移植中的表现”外科研究杂志。

Donor cell engraftment in recipient lymphoid tissues after rat limb allograft

• DOI: 10.1016/j.jss.2004.09.007
• 发表时间: 2005-03-01
• 期刊: JOURNAL OF SURGICAL RESEARCH
• 影响因子: 2.2
• 作者: Muramatsu, K;Kurokawa, Y;Kawai, S
• 通讯作者: Kawai, S

Muramatsu K: "Fate of donor cell in vascularized bone grafts"Plastic and reconstructive surgery. 111(2). 763-72 (2003)

Muramatsu K：“供体细胞在血管化骨移植物中的命运”整形和重建手术。

Neurospheres induced from bone marrow stromal cells are multipotent for differentiation into neuron, astrocyte, and oligodendrocyte phenotypes

• DOI: 10.1016/j.bbrc.2004.07.201
• 发表时间: 2004-09-24
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Suzuki, H;Taguchi, T;Kawai, S
• 通讯作者: Kawai, S

Prolonged survival of rat hindlimb allografts following short-course FK506 and mycophenolate mofetil combination therapy.

短期 FK506 和吗替麦考酚酯联合治疗后大鼠后肢同种异体移植物的存活时间延长。

• 发表时间: 2005
• 期刊: Microsurgery May 4
• 作者: Muramatsu K;Muramatsu K;Song YX
• 通讯作者: Song YX