Detection of Helicobacter sp. in bile and gallbladder tissue in patients with biliary tract diseases.

螺杆菌的检测。

• 批准号: 13671353
• 负责人: YOSHIDA Keisuke
• 金额: $ 2.18万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671353/
• 关键词: Gallbladder cancer; Hericobacter; Hericobacter infection of the biliary tract

To clarify the role of helicobacter infection in the pathogenesis of biliary tract cancer, evidence of helicobacter infection in patients who had cholecys^ectomy for the treatment of biliary tract diseases was investigated.Sixty-six patients who had biliary surgery between June 2001 and August 2002 were studied.Using removed gallbladder tissue and bile, Helicobacter specific genomic DNA was examined by Polymerase Chain Reaction (PCR) for 16S rRNA and by nested PCR for 860 bp genomic DNA fragment. 16S rRNA was positive in only one gallbladder tissue and helicobacter pylori specific 860 bp DNA fragment was detected hi 12 patients (18.2%). However, culture of gallbladder tissue and bile for helicobacter was negative in all. Histological examination revealed Helicobacter-4ikerod^brm microorganism in only one gallbladder specimen.Conclusion: Even though DNA fragment of helicobacer (probably of H.pylori) was detected in 18 % of the patients, our results did not support the presumption that we have significantly high prevalence of biliary tract helicobacter infection among patients of Niigata district where was well known as the high risk area for biliary tract cancer. However, a recent report from Tokyo described the surprisingly high incidence of helicobacter bilis specific 16S rRNA in bile of Japanese and Thai patients with biliary diseases. It was considered important to reconstruct research program focusing on H.bilis using H.bilis specific culture method and H.bilis specific PCR system.

为了阐明螺杆菌感染在胆道癌发病机制中的作用，对因治疗胆道疾病而接受胆囊切除术的患者中螺杆菌感染的证据进行了调查。 2001年6月至2002年8月期间接受胆道手术的66名患者使用取出的胆囊组织和胆汁，通过聚合酶链反应 (PCR) 检查螺杆菌特异性基因组 DNA 16S rRNA 并通过巢式 PCR 获得 860 bp 基因组 DNA 片段。仅 1 例胆囊组织中 16S rRNA 呈阳性，12 名患者（18.2%）检测到幽门螺杆菌特异性 860 bp DNA 片段。然而，胆囊组织和胆汁的螺杆菌培养均为阴性。组织学检查仅在一份胆囊标本中发现 Helicobacter-4ikerod^brm 微生物。结论：尽管在 18% 的患者中检测到了 Helicobacter-4ikerod^brm 微生物，但我们的结果并不支持我们的假设：新泻地区是众所周知的胆道癌高发区，胆道螺杆菌感染率很高。然而，东京最近的一份报告描述了日本和泰国胆道疾病患者胆汁中胆汁螺杆菌特异性 16S rRNA 的出现率惊人地高。利用H.bilis特异性培养方法和H.bilis特异性PCR系统重建以H.bilis为中心的研究计划被认为是重要的。

项目成果

Wakai T.: "Early gallbladder carcinoma does not warrant radical resection"Br J Sure. 88・5. 675-678 (2001)

Wakai T.：“早期胆囊癌不需要根治性切除”Br J Sure 88・5 (2001)。

Yasuo Tsutiya: "Genetic polymorphism of cytochrome P450 1A1 and risk of gallbladder cancer"J Exp Clin Cancer Res. 21・1. 119-124 (2002)

Yasuo Tsutiya：“细胞色素 P450 1A1 的遗传多态性与胆囊癌的风险”J Exp Clin Cancer Res 21・1 (2002)。

Yokoyama N.: "Immunohistochemically detected hepatic micrometastases predict a high risk of intrahepatic recurrence after resection of colorectal carcinoma liver"Cancer. 94・6. 1642-1647 (2002)

Yokoyama N.：“免疫组织化学检测的肝微转移预测结直肠癌肝脏切除后肝内复发的高风险”癌症 94・6 (2002)。

Yasuo Tsutiya, Sato T, Kiyouhara C, Yoshida K, Ogophi K, Nakamura K, Yamamoto M.: "Genstic polymorphism of cytochrome P450 1A1 and risk of gallbladder cancer."J Exp Clin Cancer Res. 21(1). 119-124 (2002)

Yasuo Tsutiya、Sato T、Kiyouhara C、Yoshida K、Ogophi K、Nakamura K、Yamamoto M.：“细胞色素 P450 1A1 的基因多态性与胆囊癌的风险。”J Exp Clin Cancer Res。

Wakai T.: "Early gallbladder carcinoma does not warrant radical resection"Br J Surg. 88・5. 675-678 (2001)

Wakai T.：“早期胆囊癌不需要根治性切除”Br J Surg. 88・5 (2001)。