Cell-cell communication in the ovarian tissue and its regulation by pituitary prolactin : in case of GnRH and annexin 5

卵巢组织中的细胞间通讯及其垂体催乳素的调节：以 GnRH 和膜联蛋白 5 为例

基本信息

批准号：
13660306
负责人：
HASHIMOTO Inoru
金额：
$ 2.05万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660306/
关键词：
pseudopergnancy GnRH corpus luteum prolactin annexin 5 Cell-cell communication progesterone apoptosis 卵巣黄体形成

项目摘要

In the ovarian tissue, the expression of annexin 5, a calcium-phospholipid binding protein and a novel target of GnRH, in the corpus luteum is suppressed by prolactin. During pseudopregnancy of rats in which two daily prolactin surges occur, inhibition of prolactin secretion by dopamine agonist, 2α-bromoergocriptine mesylate (CB-154), resulted in the augmentation of annexin 5 expression and the occurrence of TUNEL positive reaction in the same luteal cells. Local administration of GnRH antagonist, Cetrorelix, to unilateral ovarian bursa of a CB-154 administered pseudopregnant rat with an osmotic pump completely suppressed both apoptosis and annexin 5 expression, while they were observed in the luteal cells of the contralateral non-treated ovary. The direct effect of GnRH on luteal cells was examined by administering GnRH agonist (GnRHa) with an osmotic pump to hemilateral ovarian bursa of a pseudopregnant rat. GnRHa induced apoptosis and annexin 5 expression in the same luteal cells. Since the administration of progesterone did not suppressed apoptosis and annexin 5 expression in the CB-154 treated pseudopregnant rats, prolactin is thought to inhibit GnRH action directly in the ovary. Getting together, it is suggested that prolactin maintains the functional luteal phase at least partly by suppressing local GnRH action. (this work was published in Endocrinology 2003 144:3625-3631).

在卵巢组织中，肠胃脂素抑制了肠道蛋白5，钙磷脂结合蛋白和GnRH的新靶标的表达。在大鼠的伪孕期间，每天发生两次催乳素的潮流，多巴胺激动剂抑制催乳素分泌，2α-溴连甲基甲基酸酯（CB-154）导致膜联蛋白5表达的增强，并在同一luteal细胞中发生TUNEL阳性反应的发生。 GNRH拮抗剂cetrorelix的局部给药以渗透泵完全抑制了凋亡和膜联蛋白5的表达，同时观察到了相对非疾病的非疾病的卵巢卵形，渗透泵完全抑制了凋亡和膜联蛋白5的表达。通过用渗透泵对伪孕鼠大鼠的半侧卵巢囊来检查GNRH对黄体细胞的直接影响。 GNRHA在同一黄体细胞中诱导凋亡和膜联蛋白5的表达。由于孕酮的给药未抑制凋亡的凋亡，并且在CB-154治疗的伪孕剂大鼠中的膜毒素5表达，因此人们认为催乳素可以直接抑制卵巢中的GNRH作用。聚在一起，建议催乳素至少通过抑制局部GNRH作用至少部分地保持功能性黄体期。（这项工作发表在《内分泌学》 2003 144：3625-3631中。