Screening of the naturally nematicidal compounds against plant parasitic nematode Pratylenchus penetrans Mechanisms

天然杀线虫化合物对植物寄生线虫穿透短体线虫的筛选及其机制

基本信息

批准号：
13660107
负责人：
KIMURA Yasuo
金额：
$ 2.24万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

In the course of a screening search for new nematicidal compounds among the fungal metabolites, using bioassay method with a root-lesion nematode Pratylenchus penetrans, suitable for developing new nematicides and new lead compounds, I found the presence of active compounds in the metabolites of Penicillium bilaiae Chalabuda, Penicillium cf. simplicissimum, and an unidentified Aspergillus sp.Biosassay-guided fractionation led to the isolation of four active compounds,2,6-pyridinedicauboxylic acid,6-methoxypicolinic acid, p-hydroxyacetophenone and a new compound named pratynolene from the culture filtraste of Penicillium bilaiae Chalabuda. Three active compounds together with a related but inactive compound were also isolated from the mycelial mats of Penicillium cf.simplicissimum. Four curvuralin analogs were obtained from the culture filtrate and mycelial mats,although two of them were inactive.From these results together with bioassays with rice and lettuce seedlings,the following facts became clear.1) Active compounds are present in the culture filtrate or mycelial mats.2) Some of the nematicidal compounds shows phytotoxic activity.3) Some compounds have selectivity such as a compound shows nematicidal activity against P.penatrans but sit is inactive against C. elegance.

在筛选真菌代谢产物之间寻找新的甲状腺素化合物的过程中，使用生物测定方法与根部神经线虫pratylenchus渗透性，适合开发新的甲酸甲酸酯和新的铅化合物，我在bitirilium bilaiae chalababuda chalabababuda s，Penicillium cff的代谢物中存在活性化合物的存在。简单的质量和未鉴定的曲霉Sp.Biosassay引导的分级，导致四种活性化合物，2,6-吡啶氨基氧基酸，6-甲氧甲状腺素酸，p-羟基乙烯二苯甲酮和一种新的化合物与培养基filtraste chal chal chalaiiaiaiaiaigab of peneiai filaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiai chal chal chal酸性化合物。三种活性化合物以及相关但非活性化合物也从菌根CF的菌丝垫中分离出来。从培养的滤液和菌丝垫中获得了四个弯曲蛋白类似物，尽管其中两个是不活跃的。针对peNaTrans的甲状腺杀菌活性，但坐骨是不活跃的。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Kimura,Y., Kusano,M., Nakahara,S.: "Nematicidal compounds from the fungi"Abstract of 3^<rd>Pan Pacific Pestiside Science, (in press).

Kimura,Y.、Kusano,M.、Nakahara,S.：“来自真菌的杀线虫化合物”第 3 届泛太平洋农药科学摘要，（正在印刷中）。

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Kimura, Y.: "Nematicidal compounds from the fungi"Abstract of 3^<rd> Pan Pacific Conference. (in press). (2003)

Kimura, Y.：“来自真菌的杀线虫化合物”第三届泛太平洋会议摘要。

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Kusano, M.: "βγ-Dehydrocurvuralin and related compounds as nematicides of Pratylenchus penetrans from the fungus Aspergillus sp."Biosci. Biotechnol. Biochem. 67. 1413-1416 (2003)

Kusano，M.：“βγ-脱氢曲夫拉林和相关化合物作为来自真菌曲霉属的短体线虫的杀线虫剂。”Biosci. Biochem. 67. 1413-1416 (2003)

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Kusano,M., Nakagami,K., Fujioka,S., Kawano,T., Shimada,A., Kimura,Y.: "βγ-Dehydrocurvularin and reloated compounds as nematicide of Pratylenchus penetrans from the fungus Asergillus sp"Biosci.Biotech.Boichem.. 67. 1413-1416 (2003)

Kusano, M.、Nakagami, K.、Fujioka, S.、Kawano, T.、Shimada, A.、Kimura, Y.：“βγ-脱氢曲霉菌素和相关化合物作为来自真菌 Asergillus sp 的穿透短体线虫的杀线虫剂”Biosci。生物技术.Boichem.. 67. 1413-1416 (2003)