Molecular analysis of UV-B protection and of UV-B signaling in soybean.

大豆 UV-B 防护和 UV-B 信号传导的分子分析。

• 批准号: 13660002
• 负责人: AKADA Shinji
• 金额: $ 2.11万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660002/
• 关键词: UV-B; soybean; R2R3-MYB transcription factors; Cahalcone Synthase; nitrogen nutrient; Lotus japonicus; chalcone synthase; 紫外線UV-B; MYB転写因子; シグナル伝達; タンパクのリン酸化; 紫外線UV-B耐性機構; UV-Bシグナル伝達; Chryptochrome遺伝子; Yeast two-hybridシステム

1.Function of UV-B responsive transcription factor GmMYB29B1 in soybean.(1)GmMYB29B1(B1) was expressed in yeast was found to function as a transcriptional activator and its C-terminal region of 29 amino acid residues was both necessary and sufficient as the activator domain in the yeast system.(2)The DNA binding domain of B1(BD) expressed in E coli was used for electrophoretic mobility-shift assay(EMSA). BD interacted specifically with H-box sequence on the promoters of soybean chalcone synthase genes(Gmchs).(3)A plasmid construct of B1 coding region inserted into a legume virus vector(pCIYVV) was inoculated into broad bean. It was found that both accumulation of anthocyanin and expression of CHS were enhanced in the inoculated plans, indicating that B1 functioned as a transcriptional activator of CHS, in vivo, as well.2.Isolation of R2R3-MYB transcription factors responsive to nitrogen nutrient-limited conditions.(1)Three Lotus genes (LjMYB101,LjMYB102, and LjMYB103), coding for R2R3-MYB transcription factors responsive to nitrogen nutrient-limited conditions were isolated. In addition, a candidate for the soybean orthologue of LjMYB101 was isolated and designated GmMYB101. They all belonged to subgroup 10 of the plant R2R3-MYB superfamily.(2)LjMYB101 was responsive to nitrate only in roots, while LjMYB103 was responsive only in shoots. Considering their phylogenetic relationship, the two genes seem to have diverged through acquisition of their organ-specific responsiveness, which might have differentiated their physiological roles.(3)The DNA binding domain of LjMYB101 expressed in E.coli interacted specifically with H-box sequence and its related MYB Type IID sequences. It was suggested that LjMYB101 functioned as a transcription factor of CHS and Gln1, which also showed up-regulated expression under low nitrogen conditions.

1.发现在酵母中表达gmmyb29b1（B1）的UV-B响应转录因子GMMYB29B1的功能。（1）在酵母中表达GMMYB29B1（B1）的功能，在29个氨基酸残基的C末端区域起作用，既需要且足够作为在YEAST系统中的激活域的c-末端。电泳移动迁移测定法（EMSA）。 BD在大豆沙尔酮合酶基因（GMCHS）的启动子上特异性相互作用。（3）将插入豆类病毒载体（PCIYVV）的B1编码区域的质粒构建体接种到宽豆中。发现在接种的计划中，花青素的积累和CH的表达都得到了增强，表明B1在体内也充当CHS的转录激活剂。22。R2R3-MYB转录因子响应响应于氮营养素含量的条件。分离了对氮营养限制条件的R2R3-MYB转录因子的编码。此外，分离并指定了LJMYB101大豆直系同源物的候选者。它们都属于植物R2R3-MYB超级家族的亚组10。（2）LJMYB101仅在根中对硝酸盐有反应，而LJMYB103仅在芽中反应良好。考虑到它们的系统发育关系，这两个基因似乎通过获得器官特异性响应能力而有所不同，这可能会区分它们的生理作用。（3）在E.Coli中表达的LJMYB101的DNA结合结构域与H-box序列及其相关的MYB类型IID序列特别相互作用。有人提出，LJMYB101充当CHS和GLN1的转录因子，在低氮条件下也表现出上调的表达。

项目成果

Miyake, K., T.Ito, M.Senda, R.Ishikawa, T.Harada, M.Niizeki: "Isolation of a subfamily of genes for R2R3-MYB transcription factors showing up-regulated expression under nitrogen nutrient-limited conditions."Plant Mol.Biol.. 53. 237-245 (2003)

Miyake, K.、T.Ito、M.Senda、R.Ishikawa、T.Harada、M.Niizeki：“R2R3-MYB 转录因子基因亚家族的分离显示在氮营养限制条件下表达上调。

Miyake, Kunihiko: "Isolation of a subfamily of genes for R2R3-MYB transcription factors showing up-regulated expression under nitrogen nutrient-limited conditions"Plant Molecular Biology. 53. 237-245 (2003)

Miyake、Kunihiko：“R2R3-MYB 转录因子基因亚家族的分离显示在氮营养限制条件下表达上调”植物分子生物学。

Miyake, Kunihiko: "Isolation of a subfamily of genes for R2R3-MYB transcription factors showing up-regulated expression under nitrogen nutrient-limited conditions."Plant Molecular Biology. 53. 237-245 (2003)

Miyake、Kunihiko：“R2R3-MYB 转录因子基因亚家族的分离显示在氮营养限制条件下表达上调。”植物分子生物学。