Development of Biosensing Method Using Electrochemical AC Impedance Measurement

使用电化学交流阻抗测量的生物传感方法的开发

• 批准号: 13650884
• 负责人: KUWABATA Susumu
• 金额: $ 2.11万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13650884/
• 关键词: biosensor; AC impedance measurement; glucose oxidase; interference material; electron mediator; グルコースオキシターゼ; 複素インピーダンスプロット; 妨害物質

Cyclic voltammetry and electrochemical AC impedance measurements were made by using a glassy carbon electrode in electrolyte solution (pH 7) containing electron mediator and glucose oxidase. The electron mediators used in this study were ferrocenecarboxylic acid and osmium bipyridine complex. In case of using the electrolyte solution containing the electron mediator alone, impedance plots gave linear relation with a slope angle of 45^○, implying that diffusion of the redox species controlled the reaction rate. When glucose oxidase and glucose were added to the solution, impedance plots with a semicircle shape were obtained and its diameter decreased with an increase in concentration of glucose. This behavior indicated that the electron transfer step determined the reaction rate. Since the decrease in diameter of the semicircle of plots means an increase in the reaction rate, then this principle allowed it to establish a method to determine glucose concentration from the impedance plots.It was, furthermore, found that the AC impedance measurement was useful sensing method with avoiding infuence of interference material in the solution. The biosensor with use of ferrocenecarboxylic acid could avoid influence of ascorbic acid. If osmium bipyridine complex was used as an electron mediator, influence of uric acid as well as ascorbic acid was erased in the obtained data. Those results strongly suggested that new biosensing way would be developed with use of electrochemical AC impedance measurements.

通过在含有电子介质和氧化葡萄糖的电解质溶液（pH 7）中使用玻璃碳电极（pH 7），进行了环状伏安法和电化学交流阻抗测量。这项研究中使用的电子介体是铁甲基二羧酸和双吡啶络合物络合物。如果仅使用包含电子介体的电解质溶液，则阻抗图具有45^○斜率角度的线性关系，这意味着氧化还原物种的扩散控制了反应速率。当将葡萄糖氧化酶和葡萄糖添加到溶液中时，获得了半圆形的阻抗图，并且其直径随着葡萄糖浓度的增加而降低。该行为表明电子转移步骤确定了反应速率。由于图的半圆的直径减小意味着反应速率的增加，因此该原理可以建立一种从阻抗图确定葡萄糖浓度的方法。此外，它发现AC阻抗测量是有用的感测方法，具有避免溶液中干扰物质的影响。使用铁甲基羧酸的生物传感器可以避免抗坏血酸的影响。如果将双吡啶复合物用作电子介体，则在获得的数据中消除了尿酸以及抗坏血酸的影响。这些结果强烈表明，通过使用电化学AC阻抗测量结果将开发出新的生物传感方式。

项目成果

Kuwabata, Susumu: "Novel Utilization of Impedance Measurement for Electrochemical Biosensing Aiming at Elimination of Influence by Interference Materials"Chemistry Letter. 32(1). 52-53 (2003)

Kuwabata，Susumu：“电化学生物传感阻抗测量的新用途，旨在消除干扰材料的影响”化学快报。

Kuwabata, Susumu: "Novel Utilization of Impedance Measurement for Electrochemical Biosensing Aiming at Elimination of Influence by Interference Materials"Chemistry Letters. 32・1. 52-53 (2003)

Kuwabata，Susumu：“旨在消除干扰材料影响的电化学生物传感的阻抗测量的新用途”化学快报 32・1（2003）。

Kuwabata, Susumu: "Novel Utilization of Impedance Measurement for Electrochemical Biosensing Aiming at Elimination of Influence by Interference Materials"Chemistry Letters. 32. 52-53 (2003)

Kuwabata，Susumu：“电化学生物传感阻抗测量的新用途，旨在消除干扰材料的影响”化学快报。