Functionalization of Electrodes Using Monolayrs of Organic Thiolate Derivatives

使用单层有机硫醇盐衍生物对电极进行功能化

• 批准号: 08455397
• 负责人: KUWABATA Susumu
• 金额: $ 1.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08455397/
• 关键词: self-assembled monolayr; thiolate derivatives; microelectrodes assembly; functionalized electrode; electrocatalysis; biosensor; cholesterol; uric acid; NAD_+; NADH

In this study, investigations have been done concerning three subjects and significant results were obtained for each subject as given in the followings.1 Preparation of microelectrodes assembly by eliminating organic thiolates adsorbed on Au electrodeImmersion of an Au electrode coated with a self-assembled monolayr of alkanethiolate in KCN aqueous solution caused partial desorption of chemisorbed thiolate molecules. The desorption was initiated at pinholes of the thiolate-coated electrode, giving desorbed holes distributed homogeneously over th entire surface of the electrode. It was found that the resulting electrode exhibited electrochemical activities characteristics of a microelectrode assembly.2 Electrochemical properties of Au electrode coated with a monolayr of organic thiolates having electrochemically active substituentsTheoretical equations were successfully derived on current-voltage curves obtained by potential sweep measurements of metal electrode coated with a self-asse … More mbled monolayr of redox-active thiolates. Explicit expressions derived for the peak-current and the peak potential were similar in their forms to those published previously using the Frumkin adsorption isotherms. Furthermore, Au electrodes coated with monolayrs of thiolates having phenothiazine derivatives were prepared and their electrocatalytic oxidation of NADH were investigated in detail.3 Immobilization of enzymes on electrode surface with use organic thiolates and their electrochemical properties as biosensorsImmobilization of cholesterol oxidase, cholesterol esterase, and uricase on Au electrodes coated with a monolayr of 2-aminoethanethiolate have been achieved by using glutaraldehyde as a cross-linking agent. It was found that the electrode on which cholesterol and cholesterol esterase were immobilized posessed amperometric sensitivities to free cholesterol as well as esterified cholesterol, while the electrode having uricase exhibited sensitivities to uric acid. Their electrochemical properties as biosensors have been investigated. Less

在本研究中，对三个课题进行了研究，每个课题都获得了如下显着结果。 1 通过消除金电极上吸附的有机硫醇盐制备微电极组件浸入涂有自组装单层纳米粒子的金电极KCN 水溶液中的链烷硫醇盐引起化学吸附的硫醇盐分子的部分解吸，解吸在硫醇盐涂层电极的针孔处开始，从而产生。脱附的空穴均匀分布在电极的整个表面上，结果表明所得到的电极表现出微电极组件的电化学活性特征。2单层具有电化学活性取代基的有机硫醇盐涂覆的Au电极的电化学性能成功地推导了理论方程。通过对涂有氧化还原活性硫醇盐的自组装单层金属电极进行电位扫描测量获得的电流-电压曲线。峰值电流和峰值电位的导出形式与之前使用 Frumkin 吸附等温线发表的形式相似。此外，制备了涂有单层吩噻嗪衍生物硫醇盐的金电极，并详细研究了它们对 NADH 的电催化氧化。 3 利用有机硫醇盐将酶固定在电极表面及其作为生物传感器的电化学特性胆固醇氧化酶、胆固醇酯酶和胆固醇氧化酶的固定化通过使用戊二醛作为交联剂，在涂有单层 2-氨基乙硫醇的金电极上实现了尿酸酶的检测。发现固定有胆固醇和胆固醇酯酶的电极对游离胆固醇和酯化胆固醇具有电流敏感性。 ，而具有尿酸酶的电极显示出对尿酸的敏感性。它们作为生物传感器的电化学特性已被研究。较少的

项目成果

Ohtani, Masayuki ; Kuwabata, Susumu ; Yoneyama, Hiroshi: "Electrochemical oxidation of reduced nicotinamide coenzymes at Au electrodes modified with phenothiazine derivative monolayrs" J.Electroanal.Chem. 422 (1-2). 45-54 (1997)

大谷正之；

Ohtani, Masayuki ; Kuwabata, Susumu ; Yoneyama, Hiroshi: "Voltammetric response accompanied by inclusion of ion pairs and triple ion formation of electrodes coated with an electroactive monolayr film" Anal.Chem.69 (6). 1045-1053 (1997)

大谷正之；

M.Ohtani,et al.: "Preparation of a Microelectrode Array Using Desorption of a Self-assembled Monolayer of Hexadecylthiolate on a Gold Electrode in Cyanide Solution" Journal of Electroanalytical Chemistry. (印刷中). (1997)

M.Ohtani 等人：“利用氰化物溶液中金电极上的十六烷基硫醇盐自组装单层的解吸来制备微电极阵列”电分析化学杂志（1997 年出版）。

M.Ohtani, et al.: "Voltammetric response accompanied by inclusion of ion pairs and triple ion formation of electrodes coated with an electroactive monolayer film" Analytical Chemistry. 69(6). 1045-1053 (1997)

M.Ohtani 等人：“伏安响应伴随着离子对的包含和涂有电活性单层膜的电极的三离子形成”分析化学。

T.Nakaminami, et al.: "Electrochemical oxidation of cholesterol catalyzed by cholesterol oxidase with use of an artificial electron medeator" Analytical Chemistry. 69(13). 2367-2372 (1997)

T.Nakaminami 等人：“使用人工电子介体通过胆固醇氧化酶催化胆固醇的电化学氧化”分析化学。