Functionalization of Electrodes Using Monolayrs of Organic Thiolate Derivatives

使用单层有机硫醇盐衍生物对电极进行功能化

• 批准号: 08455397
• 负责人: KUWABATA Susumu
• 金额: $ 1.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08455397/
• 关键词: self-assembled monolayr; thiolate derivatives; microelectrodes assembly; functionalized electrode; electrocatalysis; biosensor; cholesterol; uric acid; NAD_+; NADH

In this study, investigations have been done concerning three subjects and significant results were obtained for each subject as given in the followings.1 Preparation of microelectrodes assembly by eliminating organic thiolates adsorbed on Au electrodeImmersion of an Au electrode coated with a self-assembled monolayr of alkanethiolate in KCN aqueous solution caused partial desorption of chemisorbed thiolate molecules. The desorption was initiated at pinholes of the thiolate-coated electrode, giving desorbed holes distributed homogeneously over th entire surface of the electrode. It was found that the resulting electrode exhibited electrochemical activities characteristics of a microelectrode assembly.2 Electrochemical properties of Au electrode coated with a monolayr of organic thiolates having electrochemically active substituentsTheoretical equations were successfully derived on current-voltage curves obtained by potential sweep measurements of metal electrode coated with a self-asse … More mbled monolayr of redox-active thiolates. Explicit expressions derived for the peak-current and the peak potential were similar in their forms to those published previously using the Frumkin adsorption isotherms. Furthermore, Au electrodes coated with monolayrs of thiolates having phenothiazine derivatives were prepared and their electrocatalytic oxidation of NADH were investigated in detail.3 Immobilization of enzymes on electrode surface with use organic thiolates and their electrochemical properties as biosensorsImmobilization of cholesterol oxidase, cholesterol esterase, and uricase on Au electrodes coated with a monolayr of 2-aminoethanethiolate have been achieved by using glutaraldehyde as a cross-linking agent. It was found that the electrode on which cholesterol and cholesterol esterase were immobilized posessed amperometric sensitivities to free cholesterol as well as esterified cholesterol, while the electrode having uricase exhibited sensitivities to uric acid. Their electrochemical properties as biosensors have been investigated. Less

在这项研究中，已经进行了有关三个受试者的研究，并为每个受试者获得了显着的结果。1通过消除在Au电极上吸附的有机硫醇来吸附的有机硫醇材料，以au电极的au电极涂层，用胶质溶解剂的au电极的au电极上的au电极上的溶液溶液中的碱性溶液中的alkcnotiol soperation soperation ioloyer so的剂量制备，使其对au电极的au电极进行了反应。分子。解吸是在硫醇料涂层电极的针孔上启动的，从而在电极的整个表面上均匀地分布着解吸孔。结果发现，微电极组装的暴露电极暴露的电化学活性特征。2Au电极的电化学特性，用覆盖的有机硫醇材料覆盖的有机硫醇酯覆盖的有机硫醇酸盐具有电化化理论的电化学理论方程，从而在电流曲线上成功地得出了更多的范围的量度……氧化还原活性硫醇酯。峰值电流和峰电势得出的显式表达式与先前使用frumkin添加吸附等温线的形式相似。 Furthermore, Au electrodes coated with monolayrs of thiolates having phenothiazine derivatives were prepared and their electrocatalytic oxidation of NADH were investigated in detail.3 Immobilization of enzymes on electrode surface with use organic thiolates and their electrochemical properties as biosensorsImmobilization of cholesterol oxide, cholesterol esterase, and uricase on Au通过使用戊二醛作为交联剂，已经实现了涂有2-氨基乙乙酯的单层涂层的电极。发现将胆固醇和胆固醇酯酶的电极固定在对游离胆固醇和酯化胆固醇的固定剂量敏感性上，而电极对尿酸的尿素敏感性暴露于尿酸。已经研究了它们作为生物传感器的电化学特性。较少的

项目成果

Ohtani, Masayuki ; Kuwabata, Susumu ; Yoneyama, Hiroshi: "Electrochemical oxidation of reduced nicotinamide coenzymes at Au electrodes modified with phenothiazine derivative monolayrs" J.Electroanal.Chem. 422 (1-2). 45-54 (1997)

大谷正之；

Ohtani, Masayuki ; Kuwabata, Susumu ; Yoneyama, Hiroshi: "Voltammetric response accompanied by inclusion of ion pairs and triple ion formation of electrodes coated with an electroactive monolayr film" Anal.Chem.69 (6). 1045-1053 (1997)

大谷正之；

M.Ohtani,et al.: "Preparation of a Microelectrode Array Using Desorption of a Self-assembled Monolayer of Hexadecylthiolate on a Gold Electrode in Cyanide Solution" Journal of Electroanalytical Chemistry. (印刷中). (1997)

M.Ohtani 等人：“利用氰化物溶液中金电极上的十六烷基硫醇盐自组装单层的解吸来制备微电极阵列”电分析化学杂志（1997 年出版）。

M.Ohtani, et al.: "Voltammetric response accompanied by inclusion of ion pairs and triple ion formation of electrodes coated with an electroactive monolayer film" Analytical Chemistry. 69(6). 1045-1053 (1997)

M.Ohtani 等人：“伏安响应伴随着离子对的包含和涂有电活性单层膜的电极的三离子形成”分析化学。

T.Nakaminami, et al.: "Electrochemical oxidation of cholesterol catalyzed by cholesterol oxidase with use of an artificial electron medeator" Analytical Chemistry. 69(13). 2367-2372 (1997)

T.Nakaminami 等人：“使用人工电子介体通过胆固醇氧化酶催化胆固醇的电化学氧化”分析化学。