EXPRESSION OF NEUROTROPHIC FACTORS RELATED TO PLASTICITY OF MOTONEURONS

与运动神经元可塑性相关的神经营养因子的表达

• 批准号: 13640669
• 负责人: MATSUMOTO Akira
• 金额: $ 1.22万
• 依托单位: JUNTENDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640669/
• 关键词: rat; bulbocavernosus muscle; endomysium; fibroblast; neurotrophin-3; mRNA; In situ hybridization; ニューロトロピン-3(NT-3); SNB運動ニューロン; NGFR; TrkA; TrkB; TrkC; IGF-I; BDNF; NT-3; CNTF

Motoneurons of the spinal nucleus of the bulbocavernosus of male rats innervate the perineal striated muscles bulbocavernosus and levator ani that attach to penis. They have an important role in copulatory behavior, which is sensitive to alterations in circulating levels of androgen. The perineal motoneurons are thought to be regulated by muscle-derived neurotrophic factors. To clarify regulatory mechanisms of muscle-derived neurotrophic factors, we examined expression of neurotrophic factors such as brain-derived neurotrophic factor (BDNF), neurotropnin-3 (NT-3), ciliary neurotrophic factor (CNTF) and insulin-like growth factor-1 (LGF-1) by immunohistochemistry and in situ hybridization. For immunohistochemistry, anti-BDNF,-NT-3,-CNTF and -IGF-1 were used, and for in situ hybridization, cDNAs for BGNF, NT-3,CNTF and IGF-1 were used. CNTF and NT-3 immunoreactivity was not detected in the bulbocarvenosus muscles. BDNF and IGF-1 immunoreactivtiy was partially detected in them. mRNA of NT-3 expressed in the muscle cells and fibroblasts of endomysium of the bulbocavernosus muscles. However, mRNAs of CNTF, IGF-1 and BDNF were not expressed in both of them. These results seem to indicate that mRNA of NT-3 expresses in the bulbocavernosus muscles although NT-3 is not detected in them by immunohistochemistry.

雄性大鼠鳞茎的脊柱核的运动神经元，支撑着附着在阴茎上的肌肉横纹肌肉和左旋ANI。它们在交配行为中具有重要作用，这对循环水平的改变敏感。会阴运动神经元被认为受肌肉衍生的神经营养因素调节。为了阐明肌肉衍生的神经营养因素的调节机制，我们检查了神经营养因子的表达，例如脑衍生的神经营养因子（BDNF）（BDNF），神经肉质素-3（NT-3），睫状神经肉芽纤维养分因子（CNTF）和胰岛素般的生长因子（CNTF）和胰岛素般的生长因子（CNTF） 1（LGF-1）通过免疫组织化学和原位杂交。对于免疫组织化学，使用了抗BDNF，-nt-3，-cntf和-igF-1，用于原位杂交，用于BGNF，NT-3，CNTF和IGF-1的CDNA。在球囊肌肉中未检测到CNTF和NT-3免疫反应性。在其中部分检测到BDNF和IGF-1免疫反应。 NT-3的mRNA在肌肉细胞中表达，肌肉肌中肌肉的内膜成纤维细胞。但是，CNTF，IGF-1和BDNF的mRNA均未表达。这些结果似乎表明，尽管没有通过免疫组织化学检测到NT-3，但NT-3的mRNA在鳞茎肌肉中表达。

项目成果

Matsumoto A.: "Age-dependent changes in CREB-binding protein immunoreactivity in the perineal motoneurons of aged male rats"Brain Res. (in press).

Matsumoto A.：“老年雄性大鼠会阴运动神经元中 CREB ​​结合蛋白免疫反应性的年龄依赖性变化”Brain Res。

Matsumoto A.: "Androgen regulates expression of androgen receptor protein in the perineal motoneurons of aged rats"Tabajos del Institio Cajal. LXXX. 100-101 (2003)

Matsumoto A.：“雄激素调节老年大鼠会阴运动神经元中雄激素受体蛋白的表达”Tabajos del Institio Cajal。

Matsumoto A.: "Age-dependent changes in CREB-binding protein immunoreactivity in the perineal"Brain Res. (印刷中).

Matsumoto A.：“会阴 CREB ​​结合蛋白免疫反应性的年龄依赖性变化”Brain Res。

Matsumoto A.: "Age-dependent changes in CREB-binding Protein immunoreactivity in the perineal motoneurons of aged male rats"Brain Res. (印刷中).

Matsumoto A.：“老年雄性大鼠会阴运动神经元中 CREB ​​结合蛋白免疫反应性的年龄依赖性变化”Brain Res。

Matsumoto A.: "Androgen regulates expression of androgen receptor protein in the perineal"Tabajos del Institio Cajal. LXXX. 100-101 (2003)

Matsumoto A.：“雄激素调节会阴雄激素受体蛋白的表达”Tabajos del Institio Cajal。