Clarification of significance of human brain carboxypeptidase B in cerebrospinal fluid as a diagnostic marker of dementia

阐明脑脊液中人脑羧肽酶 B 作为痴呆症诊断标志物的意义

• 批准号: 16590860
• 负责人: MATSUMOTO Akira
• 金额: $ 2.3万
• 依托单位: Foundation for Biomedical Research and Innovation
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590860/
• 关键词: cerebrospinal fluid; Alzheimer's disease; mild cognitive impairment; early diagnostic marker; mass spectrometry; neurological disease; beta amyloid; carboxypeptidase B; カルボキシペプチダーゼB; ヒト脳型カルボキシペプチダーゼB; 臨床情報データベース; 髄液

Human brain carboxypeptidase B (HBCPB) exhibits unique characteristics as a candidate marker protein for Alzheimer's disease (AD) ; its prominent expression in the hippocampus which plays significant role in memory and recognition, its in vitro dissociation activity for synthetic beta-amyloid oligomer which inhibits long-term potentiation, its physiological expression in cerebrospinal fluid (CSF), and declined expression in the hippocampus of sporadic AD brains. To explore diagnostic significance of HBCPB expression in CSF, both qualitative and quantitative analyses using mass spectrometry were performed for HBCPB peptides and an array of C-terminally truncated beta-amyloid peptides. The analyses together with clinical informations such as clinical evaluation laboratory examination and image information, statistical study in terms of early diagnostic marker is now being carried out.In the fiscal years of 2004 and 2005, 66 and 31 cases, respectively, were subjected to the study of CSF a … More nd clinical information. Before disclosure limit of clinical information, end of fiscal 2005, all CSF samples were subjected to analysis of HBCPB and bata-amyloid peptides using antibody-assisted mass spectrometric analysis by SELDI TOF-MS platform. According to the results of this analysis, cases were divided into abnormal group (abnormal HBCPB peptide pattern and low A-beta 1-42/A-beta 1-40 ratio), normal group (normal HBCPB peptide pattern and normal A-beta 1-42/A-beta 1-40 ratio) and unclassified group (rest of the above ). After the disclosure of clinical information, selection of HBCPB-derived marker peptide is now under selection and identification, and statistical analysis in terms of AD-diagnostic marker will be under investigation.On the other hand, MCI, which is regarded as the preclinical stage of AD and is actually a crucial research target in the United States, is introduced as sub-category in this study. Since definitive diagnosis of MCI retrospective in nature, to check transition of MCI cases to AD, additional 5-year follow-up study is necessary. Up to now, CSF HBCPB-peptide designated C14EP71-1 and A-beta 1-42/A-beta 1-40 ratio are two candidate markers for early diagnosis of AD/MCI and will be comparatively analyzed. Less

人脑大脑羧蛋白酶B（HBCPB）在海马吉奇（Hippocampus Jichs）中的阿尔茨海默氏病（AD）ression的候选特征是一种候选标记蛋白，在某些IGOMER中的作用显着，在某些IGOMER中的作用显着，抑制长期增强，抑制其物理学表达，在囊肿的体液中（CSF）中的某些重要性（CSF） CPB肽和一系列C截断的β-淀粉样蛋白肽以及临床评估实验室开采和图像图像信息等临床信息，现在正在阐明早期诊断标记的统计研究。在2005年和2005年，66例和31例病例中，对CSF A的研究进行了研究... 2005财年，所有CSF样品均经过HBCPB和BATA - 淀粉样蛋白肽的分析SELDI TOF-MS平台的光谱分析。 42/A-BETA 1-40比率）和未分类组（上述其余部分）。在本研究中，MCI作为AD的临床前阶段实际上是一个至关重要的研究目标。 -beta 1-42/a-beta 1-40比例是两个候选标记，用于早期诊断AD/MCI，将进行比较

项目成果

Glycolysis regulates the induction of lactate utilization for synaptic potentials after hypoxia on the granule cell of guinea pig hippocampus.

豚鼠海马颗粒细胞缺氧后，糖酵解调节突触电位的乳酸利用诱导。

• 发表时间: 2004
• 期刊: Neurosci.Res. 50(4)
• 作者: Hinokio Y;Suzuki S;Oka Y.;T.Tanaka et al.
• 通讯作者: T.Tanaka et al.

Glycolysis regulates the induction of lactate utilization for synaptic potentials after hypoxia in the granule cell of guinea pig hippocampus

• DOI: 10.1016/j.neures.2004.08.008
• 发表时间: 2004-12-01
• 期刊: NEUROSCIENCE RESEARCH
• 影响因子: 2.9
• 作者: Takata, T;Yang, B;Yokono, K
• 通讯作者: Yokono, K

Learning deficits in N279K tau transgenic mice and an assembly model of tau protein in Molecular Neurobiology of Alzheimer Disease and Related Disorders

阿尔茨海默病及相关疾病的分子神经生物学中 N279K tau 转基因小鼠的学习缺陷和 tau 蛋白的组装模型

• 发表时间: 2004
• 作者: Taizo Taniguchi;Shogo Matsuyama;et al.
• 通讯作者: et al.

Transgenic mice expressing mutant (N279K) human tau show mutation dependent cognitive deficits without neurofibrillary tangle formation.

表达突变型 (N279K) 人类 tau 蛋白的转基因小鼠表现出突变依赖性认知缺陷，但没有神经原纤维缠结的形成。

• 发表时间: 2005
• 期刊: FEBS Lett. 579
• 作者: Taniguchi;T.他
• 通讯作者: T.他

Spatial learnining of mice lacking a neuron-specific EGF family protein, NELL2,

缺乏神经元特异性 EGF 家族蛋白 NELL2 的小鼠的空间学习

• 发表时间: 2005
• 期刊: J. Pharmacol. Sci. 98(3)
• 作者: ShogoMatsumoto;Nobuyuki Doe;Naoki Kurihara et al.
• 通讯作者: Naoki Kurihara et al.