Ion mobility mass spectrometer with electrospray ionisation (ESI-IMS-MS) for protein analysis

用于蛋白质分析的电喷雾电离离子淌度质谱仪 (ESI-IMS-MS)

• 批准号: 531399310
• 金额: --
• 依托单位: Universität zu Köln
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/531399310?language=en
• 关键词: Ion; mobility; mass; spectrometer; electrospray

The newly established plant metabolic biochemistry lab at the University of Cologne uses a combination of mass spectrometry and biochemical approaches to study plant metabolic processes in the context of environmental interactions with a focus on protein and proteome analyses. The Department of Plant Metabolic Biochemistry is part of the Cluster of Excellence on Plant Sciences (CEPLAS) and will contribute proteomic approaches to various projects in cooperation with other groups of the cluster. The proposed ion mobility mass spectrometer with electrospray ionization (ESI-IMS-MS) is required for new research projects and to continue running projects at the new location. Signaling processes in the interaction of plants with their biotic and abiotic environment are the focus of research interest. In order to reliably detect and quantify the dynamics of protein abundances, the interaction of proteins with other proteins or ligands, as well as post-translational modifications at individual proteins, a highly sensitive mass spectrometer with a high resolution is required. In addition to separation by hydrophobicity (HPLC), separation by mass-to-charge (m/z) ratio (quadrupole) and mass analysis (ion trap/flight tube), IMS mass spectrometry systems have an additional module that analyses ion mobility and uses it as a further separation criterion. This fourth dimension of separation significantly increases the number of peptides analyzed and thus achieves a dramatic increase in both the number of proteins identified and the accuracy of quantification. The IMS module is furthermore required to enable the separation of molecules of identical mass based on their three-dimensional structure. The lead applicant's working group was previously based in the Department of Plant Proteomics at Leibniz Universität Hannover. Three mass spectrometers for proteome analysis were available in the department allowing the group members to gain hands-on experience and develop a broad expertise for plant proteomics.

科隆大学的新成立的植物代谢生物化学实验室结合使用质谱和生化方法的结合，在环境相互作用的背景下研究植物代谢过程，重点是蛋白质和蛋白质分析。植物代谢生物化学系是植物科学卓越群（CEPLAS）的一部分，并将与其他集群合作为各种项目贡献蛋白质组学方法。新的研究项目需要使用电喷雾电离（ESIIMS-MS）的拟议的离子移动质谱仪，并继续在新位置运行项目。植物与生物和非生物环境的相互作用中的信号传导过程是研究兴趣的重点。为了可靠地检测和量化蛋白质丰度的动力学，蛋白质与其他蛋白质或配体的相互作用以及在单个蛋白质上的翻译后修饰，需要具有高分辨率的高度敏感的质谱仪。除了疏水性分离（HPLC）外，通过质量到荷尔兰（M/Z）比（四极杆）和质量分析（离子陷阱/飞行管）分离，IMS质谱系统还具有一个额外的模块，可以分析离子迁移率，并将其用作进一步的分离标准。分离的第四维显着增加了所分析的胡椒体的数量，因此达到了IMS模块，还需要基于其三维结构来实现相同质量分子的分离。 Lead Application的工作组以前曾在LeibnizUniversitätHannover的植物蛋白质组学系设在植物蛋白质组学系。该部门提供了三个用于蛋白质分析的质谱仪，使小组成员能够获得动手经验，并为植物蛋白质组学提供广泛的专业知识。