High Sensitive Intra-operative Fluorescent Diagnosis for Extent and Metastasis of Malignant Tumors

高灵敏术中荧光诊断恶性肿瘤的范围和转移

• 批准号: 12680824
• 负责人: MATSUMURA Akira
• 金额: $ 2.05万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680824/
• 关键词: photodynamic diagnosis; fluoerscence; 5-aminolevulinic acid; malignant glioma; gastric cancer; lung cancer; sentinel diagnosis; intraoperative diagnosis; Photodunamic diagnosis; Aminolevulici asid; fluorescence; tumor diagnosis; porphyrin; na

1. GI surgery (Dr. Adachi)1) Intraoperative Fluorescence DiagnosisThree early one advanced gastric cancer patients were diagnosed by 5-ALA photodynamic diagnosis (PDD). All main lesions were positive. Lymph nodes showing fluorescence were positive for metastasis.2) Lymph node micro metastasis in advanced cancersAmong 134 lymph nodes, 35 lymph nodes were positive for PDD. Only two lesion showed pathological metastasis. However, all the PDD positive lymph nodes were positive for CK19, which indicate micro-metastasis before the pathological metastasis become evident.2. Respiratory Surgery (Dr. Ishikawa)7 cases were studied by 5-ALA tneidiated PDD (4 bronchoscopy and 3 intraoperative). Intraoperative PDD was not useful because the lesion were not on the surface. Bronchoscopic PDD was useful in defining the lesion at the wall of the trachea, which was better than autofluorescence technique.3. Neurosurgery (Dr. Matsumura, Nose, Takano)PDD was performed in 31 cases. The positive fluorescence was obtained in 23 cases (74.2%). The negative fluorescence were seen in patients with either no Gd(+) lesion or patients under corticosteroid therapy. The PDD was useful in determining the border of the tumor which was not clear with naked eyes. The pathological specimen shoed also good correlation of fluorescence and cell density and MIB-1 index, which indicate the tumor speciflticity of this method.

1.胃肠道手术（足立博士） 1）术中荧光诊断 3例早期1晚期胃癌患者通过5-ALA光动力诊断（PDD）进行诊断。所有主要病变均呈阳性。淋巴结呈荧光显示转移阳性。2）晚期癌症淋巴结微转移134个淋巴结中，35个淋巴结PDD阳性。仅两个病灶出现病理转移。但所有PDD阳性淋巴结CK19均呈阳性，提示在病理转移明显之前已存在微转移。2．呼吸外科（Ishikawa 博士）通过 5-ALA 辅助 PDD 对 7 例病例进行了研究（4 例支气管镜检查和 3 例术中）。术中 PDD 没有用，因为病变不在表面。支气管镜PDD可明确气管壁病变，优于自发荧光技术。 3．神经外科（Matsumura博士、Nose、Takano）对31例进行了PDD。荧光阳性23例（74.2%）。在没有 Gd(+) 病变的患者或接受皮质类固醇治疗的患者中观察到负荧光。 PDD 可用于确定肉眼不清楚的肿瘤边界。病理标本的荧光与细胞密度及MIB-1指数也有良好的相关性，表明该方法具有肿瘤特异性。

项目成果

松村 明, 山本哲哉, 張 天, 高野晋吾, 木村 泰, 坪井康次, 安田 貢, 能勢忠男: "高感度CCDカメラを用いた悪性脳腫瘍の蛍光ナビゲーション手術"Neuro-Oncology. 11(2). 105-111 (2001)

Akira Matsumura、Tetsuya Yamamoto、Tian Zhang、Shingo Takano、Yasushi Kimura、Koji Tsuboi、Mitsugu Yasuda、Tadao Nose：“使用高灵敏度 CCD 相机进行恶性脑肿瘤的荧光导航手术”神经肿瘤学 11(2)。 -111 (2001)

Matsumura A. et al.(分担): "In vivo and in vitro uptake study of boronated porphyrins for neutron capture therapy. In : Frontiers in Neutron Capture Therapy Vol.2, pp283-287"Hawthrone MF, Shelly K, Wiersema RJ eds, ., Plenum Press, NewYork. 1469 (2001)

Matsumura A. 等人（贡献者）：“用于中子捕获疗法的硼化卟啉的体内和体外摄取研究。见：中子捕获疗法前沿第 2 卷，第 283-287 页”Hawthrone MF、Shelly K、Wiersema RJ 编辑，.，全会出版社，纽约 1469 (2001)。

Shibata Y, Matsumura_A, Yoshida F, Yamamoto T, Nakai K, Nose T, Sakata I, Nakajima S: "The advantage of porphyrin for BNCT from the point of view of cell cycle"Frontiers in Neutron Capture Therapy Vol.2. Hawthrone MF, Shelly K. Wiersema RJ eds., Plenum Pr

Shibata Y、Matsumura_A、Yoshida F、Yamamoto T、Nakai K、Nose T、Sakata I、Nakajima S：“从细胞周期的角度来看卟啉用于 BNCT 的优势”中子捕获疗法前沿第 2 卷。

高野晋吾 ら: "肺転移をきたした小児斜台部chordoma。"Neuro-oncology. 10. 89-92 (2000)

Shingo Takano 等人：“儿童斜坡脊索瘤伴肺转移。” 神经肿瘤学 10. 89-92 (2000)

Shibata Y, Matsumura A, Nose T, (5 other authors): "Competitive uptake of porphyrin and LDL via the LDL receptor in glioma cell lines : flow cytometric analysis"Cancer Lett. 166. 79-87 (2001)

Shibata Y、Matsumura A、Nose T（其他 5 位作者）：“胶质瘤细胞系中通过 LDL 受体竞争性摄取卟啉和 LDL：流式细胞术分析”Cancer Lett。