Gene expression of the mouse reproduced without meiotic division

未经减数分裂繁殖的小鼠的基因表达

基本信息

批准号：
12680722
负责人：
KANEKO-ISHINO Tomoko
金额：
$ 2.3万
依托单位：
TOKAI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680722/
关键词：
cloned mouse anomalies in placentas imprinted genes gene expression ES cells epigenetics primordial germ cell erasing genomic imprinting memory

项目摘要

We cloned mice from the nuclei of fresh or primary cultured donor cells with defined genetic backgrounds under standardized experimental conditions. Most (99/107)clones developing to term were alive and apparently normal. Imprinted genes were all expressed "from appropriate parental alleles in both the cloned embryos and their placentas. However, " significantly reduced transcript levels for several imprinted and non-imprinted genes occurred in clone-associated placentas (which were invariably hypertrophic) although to a "minimal extent in corresponding fetuses. Transcript levels for Igf2 and H19, imprinted" "genes presumed to be prone to cloning-induced epimulation, were within the control range." "Thus, cloning by nuclear transfer does not quantitatively perturb parent-specific imprinting" "memory established during gametogenesis. Moreover, the epigenetic effects of nuclear" "transfer cloning are not stochastic, and can be traced to specific genes expressed in the" placenta. Genomic … More imprinting is an epigenetic mechanism that causes functional differences between "paternal and maternal genomes, and plays an essential role in mammalian development." Stage-specific changes in the DNA methylation patterns of imprinted genes suggest that "their imprints are erased some time during the primordial germ cell (PGC) stage, before" their gametic patterns are re-established during gametogenesis according to the sex of individuals. To define the exact timing and pattern of the erasure "process, we analyzed parental-origin-specific expression of imprinted genes and DNA" "methylation patterns of differentially methylated regions (DMRs) in embryos, each derived" from a single day -11.5 to day -13.5 PGC by nuclear transfer. Analysis of DNA methylation in day -10.5 to -12.5 PGCs demonstrated that PGC clones represented the DNA methylation status of donor PGCs well. These findings provide strong evidence that the erasure process "of genomic imprinting memory proceeds in the day -10.5 to -11.5 PGCs, with the timing" precisely controlled for each imprinted gene. Less

我们从定义的遗传背景（99/107）的新鲜或原代供体细胞的核中克隆小鼠。。在配子发生过程中建立的核心的表观遗传效应“”转移克隆不是随机的，并且可以在“胎盘机制”中表达，从而在“胎盘机制”中表达，从而在“父亲和母体基因组”之间引起功能差异，并且在哺乳动物发育中起着重要的作用。 “阶段特异性的变化在及时线细胞（PGC）阶段的一段时间的刻痕基因的DNA甲基化模式中，在“根据个体的性别，在配子发生过程中重新建立了它们的配子模式。擦除的时机和模式“过程，我们分析了胚胎中差异甲基化区域（DMR）在胚胎中的父母 - 原始基因DNA的表达”，每天都从单天-11.5到白天通过核-13.5 pgc得出。我们的PGC的转移。