Molecular changes at the posterior horn of spinal cord resulting from spinal root avulsion

脊髓根撕脱引起的脊髓后角的分子变化

基本信息

批准号：
12680733
负责人：
IWAKI Toru
金额：
$ 1.92万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680733/
关键词：
Root avulsion Heat shock protein 27 P38 MAP kinase Substantia gelatinosa GFAP glial cell line-derived neurotropic factor 神経外傷脊髄痛覚神経再生神経栄養因子アストロサイトシュワン細胞 Hsp27 ミクログリア p38

项目摘要

The aim of this study was to demonstrate acute to subacute molecular episodes in the dorsal horn following root avulsion using immunohistochemical methods. Hsp27 was accumulated selectively in the injured substantia gelatinosa 24 hours after avulsion injury. The localization of Hsp27 in astrocytes within the substantia gelatinosa was confirmed by the double immunofluorescence and by immunoelectron microscopy for Hsp27. The pattern of Hsp27 expression subsequently changed from glial pattern to punctate pattern by 7 days. Immunoelectron microscopy revealed that the punctate pattern in the subacute stage corresponded to distal parts of the astrocytic processes. We conclude that Hsp27 is a useful marker for demonstrating dorsal horn lesions following avulsion. Next, we examined temporal molecular alterations from the CNS-PNS junction to the ruptured dorsal root after traction injury. At 28 days after dorsal rupture injury, GFAP-positive structures were identified showing rather straight lines from the centro-peripheral junction toward the peripheral stump in the ruptured dorsal root. Glial cell line-derived neurotrophic factor immunoreactivity had appeared showing a process-like profile on the ruptured dorsal root by 28 days, and it was closely related with GFAP-positive structures. We therefore conclude that the astrocytes show a unique ability to extend their processes toward the stump. This ability may provide a new medium for the study of axonal regeneration in future clinical experiments.

本研究的目的是使用免疫组织化学方法证明根撕脱后背角的急性至亚急性分子发作。撕脱伤后24小时，Hsp27选择性地积聚在受伤的胶质质中。通过双重免疫荧光和 Hsp27 的免疫电子显微镜证实了 Hsp27 在星形胶质细胞中的定位。随后 7 天，Hsp27 表达模式从胶质模式变为点状模式。免疫电子显微镜显示亚急性阶段的点状图案对应于星形胶质细胞过程的远端部分。我们得出结论，Hsp27 是显示撕脱后背角损伤的有用标记。接下来，我们检查了牵引损伤后从 CNS-PNS 交界处到破裂背根的时间分子变化。背侧破裂损伤后 28 天，鉴定出 GFAP 阳性结构，显示从破裂背根的中央-外周交界处到外周残端的相当直线。 28天时，胶质细胞源性神经营养因子免疫反应性在破裂的背根上出现了突起样的特征，并且与GFAP阳性结构密切相关。因此，我们得出结论，星形胶质细胞显示出将其过程向残端延伸的独特能力。这种能力可能为未来临床实验中轴突再生的研究提供新的介质。