Analysis of receptor carbohydrates for Helicobacter pylori from human fluds

人类体液中幽门螺杆菌受体碳水化合物的分析

• 批准号: 12680619
• 负责人: KOJIMA Naoya
• 金额: $ 2.37万
• 依托单位: TOKAI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680619/
• 关键词: Helicobacter pylori; Human fluids; Glycosphingolipids; Bacterial adhesion; ルイスb型血液型糖鎖; スルファチド; 糖脂質; スフィンゴ糖脂質; 人工糖脂質; 人工糖タンパク質; α-ヒドロキシ脂肪酸

In order to investigate the receptor molecules for H.pylori in human, this study involved1)Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaecharide-conjugated neoglycoconjugates. "These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to" "ceramide, sphingomyelin, or polyacrylamides conjugated with β-galactose, lactose, 3'-" "sialyllactose or 3'-sulfo-β-galactose. Furthermore, neoglycolipids or BSA-derivatives" with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with α-hydroxyl fatty acid was much stronger than GalCer with the non-hydroxyl fatty acid.2) When direct adhesion of H. pylori to immobilized human fluids, all of the clinical "isolated strains strongly adhered to immobilized human gastric juice. On the other hand, " 2 of 5 strains did not adhered to Leb-antigen and one strain did not adhered to sulfatide. The adhesion of H. pylori to human gastric juice remarkably reduced when gastric juice "immobilized on the plate were oxidized by periodate, but that was not affected by the" "treatment with sialidase, indicating that the receptors in gastric juice are non-sialylated" "glycoconjugates. In addition, Leb-oligosaccharides, sulfatide, and sulfated" oligosaccharides did not inhibit the adhesion to gastric juice.

为了研究人类幽门螺杆菌的受体分子，本研究涉及1)将幽门螺杆菌对固定化鞘糖脂（GSL）的直接粘附与其相应的寡糖结合的新糖结合物进行比较“发现这些细菌粘附于硫苷脂。 、GM3、GalCer 和 LacCer，但不包括“神经酰胺”、鞘磷脂或与β-半乳糖、乳糖、3'-"唾液酸乳糖或3'-磺基-β-半乳糖缀合的聚丙烯酰胺此外，具有相应寡糖的新糖脂或BSA衍生物不能作为配体。幽门螺杆菌粘附到GalCer。含有α-羟基脂肪酸的GalCer比含有非羟基脂肪酸的GalCer强得多。2）直接粘合时将幽门螺杆菌与固定化人体液体进行比较后，所有临床“分离菌株均强烈粘附于固定化人体胃液。另一方面，”5 株菌株中的 2 株不粘附 Leb 抗原，1 株不粘附脑硫苷脂。当“固定在平板上的胃液被高碘酸盐氧化”时，幽门螺杆菌对人胃液的粘附力会尴尬地降低，但这并没有受到“”处理的影响。唾液酸酶，表明胃液中的受体是非唾液酸化的糖酸酯。此外，Leb-低聚糖、脑硫苷脂和硫缀合的低聚糖不抑制对胃液的粘附。

项目成果

Tang.W.: "Requirement of ceramides for adhesion of Helicobacter pylon to glycosphingolipids"FEBS Lett. 504. 31-35 (2001)

Tang.W.：“幽门螺杆菌与鞘糖脂粘附所需的神经酰胺”FEBS Lett。

Tang. W., Seino, K., Ito, M., Konishi, T., Senda, H., Makuuchi, M., Kojima, N., &Mizuochi, T: "Requirement of ceramides for adhesion of Helicobacter pylori to glycosphingolipids."FEBS Lett.. 504. 31-35 (2001)

唐。

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黑田 Y.、中田 M.、广濑 S.、白井 T.、岩本 M.、泉井 S.、小岛 N.、

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Kuroda, Y: "Abnormal IgG galactosylation in MRL-lpr/lpr mice:pathogenic roles on the development of arthriti"Pathol.Int. 51. 909-915 (2001)

Kuroda, Y：“MRL-lpr/lpr 小鼠中异常的 IgG 半乳糖基化：对关节炎发展的致病作用”Pathol.Int。