CHANGES OF THE EXPRESSION FOR THE STRESS PROTEINS IN RAT SKELETAL MUSCLES

大鼠骨骼肌应激蛋白表达的变化

基本信息

批准号：
12680041
负责人：
OISHI Yasuharu
金额：
$ 1.86万
依托单位：
KUMAMOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680041/
关键词：
heat shock proteins myosin heavy chain HSP72 muscle hypertrophy muscle atrophy fiber type composition muscle injury soleus heat shock protein ミオシン重鎖筋線維横断面積筋繊維組成ストレス蛋白質ブピバカイン筋損傷ヒラメ筋

项目摘要

We performed two experiments in 2000-2002. One is the expression of heat shock proteins (HSPs) in regenerating rat soleus muscle, and the other is the expression of HSPs in hypertrophied rat soleus muscle.(1) The expression of HSPs in regenerating rat soleus muscle : the soleus muscle fibers were degenerated by injection of bupivacain. The regenerate fibers were observed at day 4 after the injection, and the fiber area gradually increased during recovery week 4. The HSP72 levels in regenerating fibers increased consistently with the growth for the regenerating fibers. These results may suggest that the HSP72 is related to the growth for the regenerating fibers.(2) The expression of HSPs in hypertrophied rat soleus muscle : soleus muscle fibers, especially the fast-twitch fibers, were hypertrophied by clenbuterol administration in drinking water for 4 weeks. The fiber type composition and the profiles of myosin heavy chain composition were changed toward "Fast" types in the slow soleus muscle treated with clenbuterol. Coexpression of myosin heavy chain isoforms were observed in the clenbuterol-treated soleus single fibers. However, the expression level of HSP72 was dramatically decreased in hypertrophied solesu muscle. Our results clearly indicate that the HSP72 was decreased in hypertrophied soleus muscle induced by clenbuterol, and suggest that the HSP72 is not related directly to the muscle hypertrophy.

我们在 2000 年至 2002 年进行了两次实验。一是再生大鼠比目鱼肌中热休克蛋白（HSPs）的表达，二是肥大大鼠比目鱼肌中HSPs的表达。（1）再生大鼠比目鱼肌中HSPs的表达：比目鱼肌纤维退化通过注射布比卡因。注射后第4天观察到再生纤维，并且在恢复第4周期间纤维面积逐渐增大。再生纤维中的HSP72水平随着再生纤维的生长而一致增加。这些结果可能表明HSP72与再生纤维的生长有关。(2)肥大的大鼠比目鱼肌中HSP的表达：在饮用水中给予克伦特罗后，比目鱼肌纤维，特别是快肌纤维肥大。 4周。在用克伦特罗治疗的慢比目鱼肌中，纤维类型组成和肌球蛋白重链组成的分布向“快速”类型改变。在克仑特罗处理的比目鱼肌单纤维中观察到肌球蛋白重链亚型的共表达。然而，肥大的比目鱼肌中 HSP72 的表达水平显着降低。我们的结果清楚地表明，在克伦特罗诱导的比目鱼肌肥大中，HSP72 减少，并表明 HSP72 与肌肉肥大没有直接关系。