Studies on the inhibition to its biofilm formation by an antibody against the coaggregation of periodontopathic bacteria

牙周病菌共聚抗体抑制其生物膜形成的研究

• 批准号: 12672035
• 负责人: KAWAMOTO Toru
• 金额: $ 2.18万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672035/
• 关键词: periodontopatic bacteria; coaggregation factor; antibody; Fusobacterium nucleatum; bacterial biofilm; hemagglutinin; fusobacterium nucleatum; 最近バイオフィルム; 赤血球凝集因子

In this project, we analyzed the inhibition to its biofilm formation by an antibody against the coaggregation of periodontopathic bacteria. We have reported that our purified L-arginine sensitive hemagglutinin (A-HA) from Fusobacterium nucleatum ATCC10953 has a high potential hemagglutination activity. We have and characterized L-arginine sensitive hemagglutinin (A-HA) from F. necleatum ATCC10953, and reported the dominant role of A-HA in the adherence of the bacterium to streptococci.For the purpose of making a study of the effects of the inhibition to bacterial biofilm formation at periodontal pockets by the antibody as a target for A-HA, we examined a basic experimentation to the following effect.1. The A-HA rabbit polyclonal antibody inhibited not only the hemagglutination with F. nucleatum and human erythrocytes but also the coaggregation of F. nucleatum with Campybacter rectus, Porphyromonas gingivalis, Prevotella intermedia or Eikenella corrodens.2. The human IgG antibody were extracted from the serum of patients with periodontal disease inhibited the hemagglutination and the coaggregation in the same way as the case of the rabbit polyclonal antibody.3. A mouse monoclonal antibody against the A-HA was made from the hybridoma by fusion with mouse myeloid cell 31 and mouse Balb/c lymph node cell.4. The bacterial biofilm formation experiment (in vitro) which the way of co-culture of periodontopathic bacteria was used for was made to be established.It was became clear with the A-HA that it had the activity of the hemagglutination and the coaggregation. It was suggested that an anti-A-HA antibody was effective in the inhibition of the baterial biofilm formation at periodontal pockets, for the reason that the antibody inhibited the hemaglutination and the coaggregation.

在这个项目中，我们分析了针对牙周病细菌共聚集的抗体对其生物膜形成的抑制作用。我们报道，从具核梭杆菌 ATCC10953 中纯化的 L-精氨酸敏感血凝素 (A-HA) 具有很高的潜在血凝活性。我们对来自 F. necleatum ATCC10953 的 L-精氨酸敏感血凝素 (A-HA) 进行了表征，并报道了 A-HA 在细菌与链球菌粘附中的主导作用。为了通过以A-HA为靶标的抗体抑制牙周袋细菌生物膜的形成，我们检查了以下效果的基本实验： 1． A-HA兔多克隆抗体不仅能抑制具核梭菌和人红细胞的血凝反应，而且能抑制具核梭菌与直肠弯曲杆菌、牙龈卟啉单胞菌、中间普雷沃氏菌、腐蚀艾肯氏菌的共凝作用。 2．从牙周病患者血清中提取的人IgG抗体与兔多克隆抗体一样，抑制血凝和共聚。 3．将杂交瘤细胞与小鼠骨髓细胞31和小鼠Balb/c淋巴结细胞融合，制备小鼠抗A-HA单克隆抗体。 4．建立了牙周病菌共培养方式的细菌生物膜形成实验（体外），明确了A-HA具有血凝和共凝活性。表明抗A-HA抗体可有效抑制牙周袋处细菌生物膜的形成，因为该抗体抑制血凝和共凝集。

项目成果

Munemasa T., ef al.: "Adeherence of Bacteroides forsythus to host cells"Microbios. 101. 115-126 (2000)

Munemasa T.等人：“福赛拟杆菌对宿主细胞的粘附”微生物。

Munemasa T.,Takemoto T. ほか: "Adherence of Bacteroides forsythus to host cells"Microbios. 101. 115-126 (2000)

Munemasa T.、Takemoto T. 等人：“福赛拟杆菌对宿主细胞的粘附”微生物学 101. 115-126 (2000)

Munemasa T: "Adeherence of Bacteroides forsythus to host cells"Microbios. 101. 115-126 (2000)

Munemasa T：“福赛拟杆菌对宿主细胞的粘附”微生物。

Okada M: "Clinical periodontal findings and microflora profiles in children with chronic neutropenia under supervised oral hygiene"J periodontol. 72. 945-952 (2001)

Okada M：“在口腔卫生监督下患有慢性中性粒细胞减少症的儿童的临床牙周表现和微生物群谱”J periodontol。

Okada, M., et al.: "Clinical periodontal findings and microflora profiles in children with chronic neutropenia under supervised oral hygiene"J.Periodontol.. 72. 945-952 (2001)

Okada, M., 等人：“在口腔卫生监督下患有慢性中性粒细胞减少症的儿童的临床牙周表现和微生物群谱”J.Periodontol.. 72. 945-952 (2001)