Roles of Extracellular Matrix in Developmental Mechanism and Treatment of Glaucoma

细胞外基质在青光眼发育机制和治疗中的作用

基本信息

批准号：
12671732
负责人：
TAWARA Akihiko
金额：
$ 2.11万
依托单位：
University of Occupational and Environmental Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

1. We examined immunohistochemically expression of the extracellular matrixes and their metabolizing enzymes in the monkey eyes that were injected triamcinolone acetonide under the tenon capsule to study the effect of corticosteroid on the extracellular matrix in the trabecular meshwork. The results suggested that corticosteroid could increase the amount of MYOC protein in the trabecular meshwork, and that it also could increase expression of TIMP-2 in the trabecular meshwork and MMP-3, MMP-9 and TIMP-2 in the ciliary body.2. We immunohistochemically stained the extracellular matrixes in the trabecular meshwork obtained by trabeculectomy from a patient with corticosteroid-induced glaucoma. The results indicated that MYOC protein might increase in the glaucomatous cornea but not in the trabecular meshwork. The results also showed that both heparan sulphate-type proteoglycan and type IV collagen increase in the trabecular meshwork. With double staining, MYOC protein was co-localized with … More type VI collagen in the tissue.3. We examined immunohistochemically localization of MMPs and TIMPs in the normal rat eyes. The results indicated that MMP-9 and TIMP-1 are localized in the cornea, MMP-9 and TIMP-2 are in the trabecular meshwork, and MMP-9, TIMP-1 and TIMP-3 are in the ciliary body.4. We examined immunohistochemically the effect of anti-glaucoma drug of nipradilol, both α- and β-blocker, on MMPs and TIMPs in rat eyes. From this study, it is indicated that nipradilol increases the amount of MMP-2 and MMP-9, and TIMP-1, and decreases TIMP-2 in the trabecular meshwork.5. We examined histologically, immunohistochemically and with in situ hybridization the trabecular tissue from rabbit with experimental anterior segment ischemia to investigate the mechanism of neovascularization in the anterior segment of the eye. The results suggested that vascular endothelial growth factor (VEGF) plays an important role in development of neovascularization in the anterior segment of the eye. Less

1.我们通过免疫组化检测了Tenon囊下注射曲安奈德的猴眼细胞外基质及其代谢酶的表达，以研究皮质类固醇对小梁网细胞外基质的影响。小梁网中 MYOC 蛋白的量，并且还可以增加小梁网中 TIMP-2 的表达2．对皮质类固醇诱发性青光眼患者小梁切除术中获得的小梁网细胞外基质进行免疫组织化学染色，结果表明MYOC蛋白可能增加。结果还表明，硫酸乙酰肝素型蛋白多糖和青光眼角膜中都没有。 IV 型胶原蛋白在小梁网中增加。通过双重染色，MYOC 蛋白与组织中的 VI 型胶原蛋白共定位。3. 我们检查了正常大鼠眼中 MMP 和 TIMP 的免疫组织化学定位。 -9 和 TIMP-1 位于角膜，MMP-9 和 TIMP-2 位于小梁网，MMP-9、TIMP-1 和TIMP-3位于睫状体中。4．我们用免疫组织化学方法检测了抗青光眼药物尼普地洛（α-阻滞剂和β-阻滞剂）对大鼠MMPs和TIMPs的影响。 5. 我们检查了小梁网中 MMP-2 和 MMP-9 以及 TIMP-1 的量，并减少了 TIMP-2。采用组织学、免疫组织化学和原位杂交的方法，对实验性眼前节缺血的家兔小梁组织进行研究，研究眼前段新生血管形成的机制，结果提示血管内皮生长因子（VEGF）在眼前节的发生发展中发挥着重要作用。眼前节新生血管较少。