MOLECULAR BIOLOGIC STUDY ON CORTICOSTEROID-INDUCED GLAUCOMA

皮质类固醇诱发青光眼的分子生物学研究

• 批准号: 07671925
• 负责人: TAWARA Akihiko
• 金额: $ 1.47万
• 依托单位: WAKAYAMA MEDICAL COLLEGE (1996)Kyushu University (1995)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671925/
• 关键词: corticosteroid-induced glaucoma; triamcinolone acetonide; histochemistry; extracellular matrix; Proteoglycans; exfoliation syndrome; goniodysgenetic glaucoma; neovascular glaucoma; ステロイド縁内障; 隅角発育異常縁内障; 血管新生縁内障; 接着分子; 免疫組織化学

We performed subtenon injection of 20 or 40 mg triamcinolone acetonide into right eyes of Japanese rabbits, and measured their intraocular pressures periodically for 4 weeks. The anterior chamber angle tissues obtained from the animals were examined histologically and histochemically using alcian blue or cupromeronic Blue. There was no significant increase of the intraocular pressure compared to control left eyes. No histological or histochemical change was detected in the trabecular tissues from corticosteroid injected eyes. The results suggest that there may be some difference in reactivity to corticosteroid between human and rabbit eyes.The effects of anterior segment ischemia on angiogenesis in the anterior segment of the eye were investigated. We cauterized both of the long posterior ciliary arteries in the right eye of 14 rabbits. The eyes were enucleated 1,2,4,7,9 and 14 days after cauterization and were studied with light microscopy. The localization of vascular endothelial gro … More wth factor (VEGF) protein and the expression of VEGF mRNA in the tissues were studied. Neovascularization on the anterior surface of the iris and in the trabecular meshwork was detected. No histological changes were observed in the retina. VEGF protein was detected in the epithelial and vascular cells of the iris. Expression of VEGF mRNA was detected in the epithelial cells of the ciliary body. These results suggest that anterior segment ischemia causes neovascularization in the anterior segment of the eye.The trabecular tissues from 4 enucleated human eyes with exfoliation syndrome were examined histologically and immunohistochemically. Exfoliation fibers were revealed to contain chondroitin sulfate dermatan sulfate and heparan sulfate-type proteoglycans as well as HNK-I epitope, while microfibrils in the extracellular matrix did not. The results suggest that depositions of proteoglycans and other macromolecules on the microfibrils may be closely associated with the formation of exfoliation materials.We examined morphologically the ratio of the ciliary body band width to the width of the posterior limbus, termed the theoretical C/L ratio, of enucleated human eyes, including those from premature infants as well as from older subjects. We also examined clinically the appearance of the ciliary body band on gonioscopy in patients having glaucoma with open iridocorneal angles, and in age mathched controls. The theoretical C/L ratio was 1.4(]SY.+-。[)2.7% in premature infants, 24.0(]SY.+-。[)5.4% in children and 36.7(]SY.+-。[)8.0% in the older subjects. On gonioscopy, 77.8% glaucomatous eyes and 13.8% control eyes showed either an invisible or very narrow ciliary body band. The results indicate that an invisible or very narrow ciliary body band represents an nuderdevelopment of the angle. Less

我们将20或40毫克曲安奈德进行眼筋膜下注射到日本兔的右眼中，并定期测量其眼压，使用阿新蓝或铜色蓝对从动物中获得的前房角组织进行组织学和组织化学检查。与对照左眼相比，眼内压没有显着增加，在小梁组织中未检测到组织学或组织化学变化。结果表明，人眼和兔眼对皮质类固醇的反应可能存在一些差异。我们研究了眼前段缺血对眼前段血管生成的影响。烧灼后 1、2、4、7、9 和 14 天摘除 14 只兔子的右眼，并用光学显微镜研究定位。研究了血管内皮生长因子 (VEGF) 蛋白的变化以及组织中 VEGF mRNA 的表达，在视网膜中未观察到 VEGF 的组织学变化。在虹膜上皮细胞和血管细胞中检测到 VEGF mRNA 在睫状体上皮细胞中表达。眼前节缺血导致眼前节新生血管形成。对4例患有剥脱综合征的摘除人眼的小梁组织进行组织学和免疫组织化学检查，发现剥脱纤维含有硫酸软骨素硫酸皮肤素和硫酸乙酰肝素型蛋白聚糖以及HNK。 -I 表位，而细胞外基质中的微纤维则没有。蛋白聚糖和其他大分子在微纤维上的沉积可能与剥落材料的形成密切相关。我们从形态学上检查了去核的睫状体带宽度与后缘宽度的比率，称为理论C/L比。我们还通过房角镜检查对患有开放性青光眼的患者的睫状体带的外观进行了临床检查。虹膜角膜角，在年龄计算的对照中，理论 C/L 比率为 1.4(]SY.+-。早产儿为 [)2.7%，儿童为 24.0(]SY.+-.[)5.4%，老年受试者为 36.7(]SY.+-.[)8.0%。在房角镜检查中，77.8% 患有青光眼，13.8% 患有青光眼。对照眼睛显示不可见或非常窄的睫状体带。结果表明不可见或非常窄的睫状体带代表角的裸露发育。较少的

项目成果

Toshiaki Kubota, Akihiko Tawara, et al.: "Neovascular tissue in the intertrabecular spaces in eyes with neovascular glaucoma" Brit J Ophthalmol. 80-8. 750-754 (1996)

Toshiaki Kubota、Akihiko Tawara 等人：“新生血管性青光眼眼小梁间隙中的新生血管组织”Brit J Ophthalmol。

Tawara A.,et al.: "Distribution and characterization of proteoglycans associated with exfoliation material." Curr.Eye Res.15・11. 1101-1111 (1996)

Tawara A., et al.：“与剥落材料相关的蛋白多糖的分布和表征。”Curr.Eye Res.15・11 (1996)。

Kaoru Motomatsu, Toshiaki Kubota, Akihiko Tawara: "Correlation between optic disc parameters and visual field in glaucoma by laser scanning tomography" Jpn J Clinic Ophthalmol. 50-4. 525-533 (1996)

Kaoru Motomatsu、Toshiaki Kubota、Akihiko Tawara：“激光扫描断层扫描青光眼视盘参数与视野之间的相关性”Jpn J Clinic Ophthalmol。

Akihiko Tawara, et al.: "Distribution and characterization of proteoglycans associated with exfoliation material." Curr Eye Res. 15-11. 1101-1111 (1996)

Akihiko Tawara 等人：“与剥落材料相关的蛋白多糖的分布和表征。”

Kubota T., Tawara A., et al.: "Neovascular tissue in the intertrabecular spaces in eyes with neovascular glaucoma" Brit. J. Ophthalmol.80・8. 750-754 (1996)

Kubota T., Tawara A., et al.：“新生血管性青光眼眼内小梁间隙中的新生血管组织”Brit. J. Ophasemol.80·8 (1996)。