Analysis of a novel human protein WDC146 highly expressed in testis : for elucidation of the function during spermatogenesis
分析睾丸中高表达的新型人类蛋白质WDC146:阐明精子发生过程中的功能
基本信息
- 批准号:12671602
- 负责人:
- 金额:$ 2.18万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1) WDC146 is a novel human protein of 146 kDa which is expressed highly in testis. It is composed of three domains, a WD40 domain, a collagen-like domain and a GPR-rich repeat domain, from N-terminus to C-terminus, respectively.2) WDC146 is highly expressed in testis both in human and mouse. The amino acid sequence showed significant homology between them overall (96%), including complete match (100%) for the region of WD40 domain. Even in Drosophila and yeast, we identified the sequence conserved in part.3) WDC146 showed marked expression in pachytene spermatocytes, and nuclear and perinuclear localization was observed intracellularly.4) The detailed analysis of the expression pattern of WDC146 during spermatogenesis is in progress, with the technical difficulty of fixation of testis applicable for in situ hybrization.5) WD40 domain is known to work as the platform motif as the time of protein-protein interaction. The two-hybrid screening was executed repeatedly to detect interactive protein with WDC146, but not yet succeed.6) Cancer-testis genes are defined as testis-specific genes also expressed in many tumors and tumor cell lines. WDC146 may be classified in them, as tumor cell lines tested all showed moderate expression of WDC146, though some tumors did not.7) The WDC146 gene is mapped at 2q14-21 and consists of 22 exons. Besides the intact mRNA of about 4kb, a chimeric one result from mis-splicing always detected at about 1kb size.8) The promoter region was identified within 0.5kb upstream region of exon 1.
1) WDC146是一种146 kDa的新型人类蛋白,在睾丸中高表达。它由三个结构域组成,从N端到C端分别是WD40结构域、类胶原蛋白结构域和富含GPR的重复结构域。2)WDC146在人和小鼠的睾丸中高表达。氨基酸序列总体上显示出显着的同源性(96%),包括WD40结构域区域的完全匹配(100%)。即使在果蝇和酵母中,我们也鉴定了部分保守的序列。3)WDC146在粗线期精母细胞中显示出显着表达,并且在细胞内观察到核和核周定位。4)对WDC146在精子发生过程中的表达模式的详细分析正在进行中,具有适用于原位杂交的睾丸固定的技术难度。5) WD40 结构域已知可作为平台基序。蛋白质-蛋白质相互作用。重复进行双杂交筛选以检测与WDC146的相互作用蛋白,但尚未成功。6)癌症睾丸基因被定义为睾丸特异性基因,也在许多肿瘤和肿瘤细胞系中表达。 WDC146 可以归入其中,因为测试的肿瘤细胞系均显示出 WDC146 的中等表达,尽管有些肿瘤没有。7) WDC146 基因定位于 2q14-21,由 22 个外显子组成。除了约 4kb 的完整 mRNA 外,错误剪接产生的嵌合体总是在约 1kb 大小处检测到。8) 在外显子 1 上游 0.5kb 区域内鉴定出启动子区域。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
ItO,S.: "A Novel WD40 Repeat Protein, WDC146, Highly Expressed during Spermatogenesis in a Stage-Specific Manner"Biochem.Biophys.Res.Commun.. 280(3). 656-663 (2001)
ItO,S.:“一种新型 WD40 重复蛋白,WDC146,在精子发生过程中以特定阶段的方式高度表达”Biochem.Biophys.Res.Commun. 280(3)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ito, S.: "A novel WD4O repeat protein, WDC146, highly expressed during spermatogenesis in a stage-specific manner"Biochem.Biophys.Res.Commun.. 280. 656-663 (2001)
Ito, S.:“一种新型 WD4O 重复蛋白 WDC146,在精子发生过程中以特定阶段的方式高度表达”Biochem.Biophys.Res.Commun. 280. 656-663 (2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ito S: "A novel WD40 repeat protein, WDC146, highly expressed during spermatogenesis in a stage-specific manner"BBRC. 280. 656-663 (2001)
Ito S:“一种新型 WD40 重复蛋白 WDC146,在精子发生过程中以特定阶段的方式高度表达”BBRC。
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SAKAI Akiko其他文献
DEVELOPMENT AND VALIDATION OF A LARGE-SCALE GLACIER MODEL BASED ON AN ENERGY BALANCE APPROACH OVER CENTRAL EUROPE
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10.2208/jscejhe.75.2_i_919 - 发表时间:
2019 - 期刊:
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KANAE Shinjiro
SAKAI Akiko的其他文献
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18K06479 - 财政年份:2018
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17K01955 - 财政年份:2017
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23510267 - 财政年份:2011
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Grant-in-Aid for Scientific Research (C)
A study of practical and effective psychological supports between the early stage after the disaster and the revival stage in the Great East Japan Earthquake
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22592367 - 财政年份:2010
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Functional analysis of chromatin dynamics during aging by genomic approach
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22710183 - 财政年份:2010
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Proteomic analysis of oxidative stress-responsive proteins in metabolic syndrome
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20510192 - 财政年份:2008
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19570017 - 财政年份:2007
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18592348 - 财政年份:2006
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15570010 - 财政年份:2003
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$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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