Synaptogenesis and plasticity requires neurotrophic factors

突触发生和可塑性需要神经营养因子

基本信息

批准号：
12671485
负责人：
HAMAKAWA Toshiro
金额：
$ 2.11万
依托单位：
Miyazaki Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671485/
关键词：
neural developmehnt calucium Lymnaea soma-soma synapse neurotrophic foctors receptor tyrosine kinase 神経節形成過程細胞内カルシウム Lymnaea stagnalis 培養神経細胞 Lymnaca stagnalis

项目摘要

The accumulation of calucium (Ca) plays important role in neural development and plasticity. The role of neurotrophic foctors (NF) for them has yet to be determined. We investigated whether NF accumulate of Ca in neural development and plasticity using Ca imaging.Somata of identified presynaptic and postsynaptic neurons were isolated from the CNS of Lymnaea and cultured in a soma-soma configuration in the presence (CM) or absence (DM) of NF. RpeD1 and VD2/3 were used for formation of excitatory synapse, RpeD1 and VD4 were used for formation of inhibitory synapse. Fura-2 was injected into somata for Ca imaging. They were cultured for 24 hours.In CM, the excitatory synapse was formed and the accumulation of Ca was seen. They were also blocked receptor tyrosine kinase inhibitors (lavendustine A), but not by inactive analogus (lavendustine B). In DM, the excitatory synapse was not developed and the accumulation of Ca was not seen. Both in CM and DM, inhibitory synapse was developed and Ca was accumulated.These results demonstrate that NF were required for the accumulation of Ca in excitatory synaptogenesis and plasticity is mediated by receptor tyrosine kinases. The development of inhibitory synapse did not require exogenous NF.

钙（Ca）的积累对神经发育和可塑性起着重要作用。神经营养因子（NF）对它们的作用尚未确定。我们使用 Ca 成像研究了 NF 在神经发育和可塑性中是否积累了 Ca。从 Lymnaea 的 CNS 中分离出已识别的突触前和突触后神经元的体细胞，并在存在 (CM) 或不存在 (DM) 的情况下以体体-体体结构进行培养。 NF。 RpeD1和VD2/3用于兴奋性突触的形成，RpeD1和VD4用于抑制性突触的形成。将 Fura-2 注射到体细胞中进行 Ca 成像。培养24小时。CM中，兴奋性突触形成，Ca积累。它们也被受体酪氨酸激酶抑制剂（lavendustine A）阻断，但不被无活性的类似物（lavendustine B）阻断。在DM中，兴奋性突触未发育并且没有观察到Ca的积累。在 CM 和 DM 中，抑制性突触均发育且 Ca 积累。这些结果表明，兴奋性突触发生中 Ca 的积累需要 NF，并且可塑性由受体酪氨酸激酶介导。抑制性突触的发育不需要外源性神经纤维。