Genetic Analysis and Expression of Senescence Marker PROTEIN-30 (SMP30) Decreasing Androgenindependenty with AGE in the Rat Liver.

衰老标记蛋白 PROTEIN-30 (SMP30) 的遗传分析和表达降低大鼠肝脏中 AGE 的雄激素非依赖性。

• 批准号: 04836027
• 负责人: FUJITA Toshiko
• 金额: $ 1.28万
• 依托单位: Tokyo Metropolitan Institu t of Gerontology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04836027/
• 关键词: Aging; Senescence; Senescence Marker Protein-30; SMP30; Regicalucin; Calucium-Binding Protein; Liver; Kidney; cDNA; 老化指標蛋白質; ラット肝臓タンパク質; 遺伝子発現; 加齢

We discovered a novel rat liver protein, the amount of which decreased androgen-independently with aging. We designated this protein, whose molecular mass was 30 KDa and pI vaue was 4.9, as senescence marker protein -30 (SMP30). The localization of SMP30 was restricted to liver and Kidney among numerous organs tested. We have isolated and characterized two cDNA clones, one consisted of 1588-bp nucleotides and other of 1195-bp nucleotides generated by alternative polyadenylation. Northern hybridization analysis showed two species of mRNA (1.7-kb and 1.4-kb) in the liver and kidney. The open reading frame encodes 299 amino acids. SMP30 is widely conserved among higher animals.

我们发现了一种新型大鼠肝脏蛋白，其数量随着衰老而减少，与雄激素无关。我们将该蛋白命名为衰老标记蛋白-30（SMP30），其分子量为30 KDa，pI值为4.9。在众多测试的器官中，SMP30 的定位仅限于肝脏和肾脏。我们分离并表征了两种 cDNA 克隆，一种由 1588 bp 核苷酸组成，另一种由选择性多聚腺苷酸化产生的 1195 bp 核苷酸组成。 Northern 杂交分析显示肝脏和肾脏中有两种 mRNA（1.7-kb 和 1.4-kb）。开放阅读框编码299个氨基酸。 SMP30 在高等动物中广泛保守。

项目成果

Fujita, T., Shirasawa, T., Columbano, A.and Maruyama, N: "Gene expression of senescence marker protein-30 (SMP30)" Biomedical Gerontology. 17. 100-101 (1993)

Fujita, T.、Shirasawa, T.、Columbano, A. 和 Maruyama, N：“衰老标记蛋白 30 (SMP30) 的基因表达”生物医学老年学。

Fujita, T., Shirasawa, T.and Maruyama, N: "Genetic analysis and expression of senescence marker protein-30 (SMP30) decreasing androgen-independently with age in the rat liver" (in press). (1993)

Fujita, T.、Shirasawa, T. 和 Maruyama, N：“衰老标记蛋白 30 (SMP30) 的基因分析和表达与大鼠肝脏中的年龄无关地随年龄的增长而降低”（出版中）。

Fujita Toshiko: "Isolation of cDNA clone encoding rat senescence marker protein-30(SMP30) and its tissue distribution" Biochemi.Biophys.Acta. 1132. 297-305 (1992)

Fujita Toshiko：“编码大鼠衰老标记蛋白-30（SMP30）的 cDNA 克隆的分离及其组织分布”Biochemi.Biophys.Acta。

Fujita Toshiko: "Isolation of cDNA clone encoding rat senescence marker protein-30(SMP30) and its tissue distribution" Biochimica et Biophysica Acta. 1132. 297-305 (1992)

Fujita Toshiko：“编码大鼠衰老标记蛋白 30（SMP30）的 cDNA 克隆的分离及其组织分布”Biochimica et Biophysicala Acta。

藤田敬子: "老化マーカー蛋白質SMP30の老化における意義" 生化学(社団法人日本生化学会). (印刷中). (1993)

Keiko Fujita：“衰老标记蛋白 SMP30 在衰老中的意义”生物化学（日本生物化学会）（出版中）。