Detection of bone marrow micrometastasis in gastrointestinal cancer patients by immunomagnetic separation

免疫磁珠分离检测胃肠道肿瘤患者骨髓微转移

• 批准号: 12671271
• 负责人: NAKAMURA Tsutomu
• 金额: $ 1.92万
• 依托单位: TOKYO WOMEN'S MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671271/
• 关键词: Micrometastasis; Immunomagnetic separation; Bone marrow; Cytokeratin; Gastric cancer; Esophageal cancer; Hematogenous metastasis; 胃癌; 食道癌; 大腸癌; 免疫磁気細胞分離; 癌細胞; 末梢血; 血行転移; ダイナビーズ

Detection of micrometastasis to the bone marrow can predict widespread disease and a poor prognosis of cancer patients. The purpose of this study was to evaluate the clinical significance of detecting micrometastasis in the bone marrow of gastrointestinal cancer patients. Bone marrow and peripheral blood samples were obtained from 53 gastric and 52 esophageal cancer patients at the time of surgery. These samples were enriched by immunomagnetic separation and immunostained with an anti-cytokeratin antibody. Expression of vascular endothelial growth factor (VEGF) human epidermal growth factor 2 (HER2), and cyclin D1 was examined in the primary tumors. Cytokeratin-positive cancer cells were observed in the bone marrow of 16 (30%) in gastric cancer patients and 13 (25%) in esophageal cancer patients. The presence of bone marrow micrometastasis was correlated with lymph node metastasis (pN) but not associated with depth of tumors (pT). Detection of bone marrow micrometastasis was not associated with peritoneal dissemination in gastric cancer. Cumulative survival of patients with bone marrow micrometastasis was significantly lower than that of patients without it in esophageal cancer. Hematogenous recurrence was more frequent in patients with bone marrow micrometastasis than in those without it. The patients who had local or dissemination recurrence did not have bone marrow micrometastasis. Detection of cancer cells in the bone marrow might be an indicator of early hematogenous metastasis in gastrointestinal cancer patients. Intensive postoperative chemotherapy seems to be indicated for these patients.

检测骨髓微转移可以预测癌症患者的广泛疾病和不良预后。本研究的目的是评估检测胃肠道癌症患者骨髓微转移的临床意义。手术时采集了 53 名胃癌患者和 52 名食管癌患者的骨髓和外周血样本。这些样品通过免疫磁性分离进行富集并用抗细胞角蛋白抗体进行免疫染色。检查原发肿瘤中血管内皮生长因子 (VEGF)、人表皮生长因子 2 (HER2) 和细胞周期蛋白 D1 的表达。在 16 名胃癌患者（30%）和 13 名食道癌患者（25%）的骨髓中观察到细胞角蛋白阳性癌细胞。骨髓微转移的存在与淋巴结转移（pN）相关，但与肿瘤深度（pT）无关。骨髓微转移的检测与胃癌的腹膜播散无关。食管癌中存在骨髓微转移的患者的累积生存率显着低于无骨髓微转移的患者。有骨髓微转移的患者比无骨髓微转移的患者更容易发生血行复发。局部或播散复发的患者没有骨髓微转移。骨髓中癌细胞的检测可能是胃肠道癌症患者早期血行转移的指标。这些患者似乎需要进行强化术后化疗。

项目成果

Tsutomu Nakamura: "Detection of bone marrow micrometastasis in esophageal cancer patients"Oncology Reports. (2004)

Tsutomu Nakamura：“食管癌患者骨髓微转移的检测”肿瘤学报告。

Matsunami Katsuhiro: "Detection of bone marrow micrometastasis in gastric cancer patients"Annals of Surgical Oncology. 10. 171-175 (2003)

松南胜宏：《胃癌患者骨髓微转移的检测》《肿瘤外科年鉴》。