Gene analysis in gastric cancer using with laser captured micro-dissection

使用激光捕获显微切割进行胃癌基因分析

• 批准号: 12671209
• 负责人: ICHIKAWA Wataru
• 金额: $ 2.3万
• 依托单位: Saitama Medical School (2001-2002)Tokyo Medical and Dental University (2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671209/
• 关键词: LCM; Cancer; TS; DPD; 抗癌剤感受性; thymidylate synthase; 大腸癌; 遺伝子多型; レーザーマイクロダイセクション; 胃癌; real-time PCR

In this study, gene analysis in gastric and colorectal cancer using with laser-captured microdissection to avoid the contamination of normal interstitial tissues.1) Cyclooxygenase-2 (COX-2):Level of COX-2 mRNA expression in the colorectal adenomas were correlated with their size, and no relationships were demonstrable between COX-2 expression and adenoma location and degree of dysplasia.2) Thymidine phosphorylase (TP):No relationship between TP expression and clinicopathological features was observed in colorectal cancer. However, the prognosis of patients with TP positive expression in both cancer and stroma was worse.3) Thymidylate synthase (TS) and Dihydropyrimidine dehydrogenase (DPD):TS and DPD gene expressions were different among primary colorectal tumors and metastatic tumors, such as hepatic metastasis. Patients with tumors of low TS and low DPD responded to fluorouracil based chemotherapy.In conclusion, gene analysis with laser-captured microdissection is the useful method.

在本研究中，使用激光捕获显微切割对胃癌和结直肠癌进行基因分析，以避免正常间质组织的污染。 1) 环氧合酶-2 (COX-2)：结直肠腺瘤中 COX-2 mRNA 表达水平相关COX-2 表达与腺瘤位置和不典型增生程度之间没有关系。 2) 胸苷磷酸化酶 (TP)：TP 之间没有关系观察结直肠癌中的表达和临床病理特征。然而，癌和间质中TP均阳性表达的患者预后较差。 3）胸苷酸合酶（TS）和二氢嘧啶脱氢酶（DPD）：TS和DPD基因在原发性结直肠肿瘤和转移性肿瘤（如肝肿瘤）中的表达存在差异。转移。低 TS 和低 DPD 的肿瘤患者对基于氟尿嘧啶的化疗有反应。 总之，激光捕获显微切割进行基因分析是有用的方法。

项目成果

Fukushima M, Okabe H, Takechi T, Ichikawa W, Hirayama R.: "Induction of thymidine phosphorylase by interferon and taxanes occurs only in human cancer cells with low thymidine phosphorylase activity"Cancer Lett. 198巻1-2号. 103-110 (2002)

Fukushima M、Okabe H、Takechi T、Ichikawa W、Hirayama R.：“干扰素和紫杉烷对胸苷磷酸化酶的诱导仅发生在胸苷磷酸化酶活性较低的人类癌细胞中”，《癌症通讯》198，第 1-2 期。 103-110（2002）

Y Shirota, J Stoehimacher, J Brabender, W Ichikawa et al.: "EROC1 and thyrnidylate synthase mRNA levels predict survival for coloredtal cancer patients receiving combination oxaliplatin chemothrapy"Journal of Clinical Oncology. 19・23. 4298-4303 (2001)

Y Shirota、J Stoehimacher、J Brabender、W Ichikawa 等人：“EROC1 和胸苷酸合酶 mRNA 水平预测接受联合奥沙利铂化疗的结直肠癌患者的生存”《临床肿瘤学杂志》19・23 (2001)。

Ichikawa, W., Uetake, H., Shirota, Y., Yamada, H., Nishi, N., Nihei, Z., Sugihara, K., and Hirayama, R.: "Combination of Dihydropyrimidine Dehydrogenase and Thymidylate Synthase Gene Expressions in Primary Tumors as Predictive Parameters for the Efficacy

Ichikawa, W.、Uetake, H.、Shirota, Y.、Yamada, H.、Nishi, N.、Nihei, Z.、Sugihara, K. 和 Hirayyama, R.：“二氢嘧啶脱氢酶和胸苷酸合酶基因的组合

H Yaniada, W Ichikawa, H Uetake, et al.: "Thymidylate synthase gene expression in primary colorectal cancer and metastatic site"Clinical Colorectal Cancer. 1・3. 169-173 (2001)

H Yaniada、W Ichikawa、H Uetake 等：“原发性结直肠癌和转移部位的胸苷酸合成酶基因表达”临床结直肠癌1·3（2001）。

Shirota Y, Ichikawa W, et al.: "Intratumoral dihydropyrimidine dehydrogenase messenger RNA level reflects tumor progression in human colorectal cancer"Ann Surg Oncol. 9巻6号. 599-603 (2002)

Shirota Y、Ichikawa W 等人：“瘤内二氢嘧啶脱氢酶信使 RNA 水平反映了人类结直肠癌的肿瘤进展”Ann Surg Oncol，第 9 卷，第 6 期。599-603 (2002)