Cell Cycle Dependence of Boron Uptake in Boron Compounds Used for Clinical Neutron Capture Therapy

用于临床中子捕获治疗的硼化合物中硼吸收的细胞周期依赖性

• 批准号: 12670852
• 负责人: YOSHIDA Fumiyo
• 金额: $ 1.09万
• 依托单位: UNIVERSITY OF TSUKUBA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670852/
• 关键词: cell cycle; flow cytometry; sodium borocaptate; boronophenylalanine; neutron capture therapy; cell cycle

In neutron capture therapy, it is important to incorporate boron in tumor cells selectively. The present study used flow cytometry to sort the cells by G0/G1 phase and G2/M phase, and the boron concentration of each fraction was measured with ICP-AES. The results revealed that BSH and BPA, had higher rates of boron uptake in the G2/M than in the G0/G1. However, the difference was more prominent with BPA. The G2/M : G0/G1 ratio decreased as a function of time in culture in BPA, thereby indicating the cell cycle dependency of BPA uptake. Such heterogeneity of boron uptake in tumor cells should be considered for microdosimetry.

在中子捕获疗法中，将硼选择性地掺入肿瘤细胞中非常重要。本研究使用流式细胞仪将细胞按G0/G1期和G2/M期进行分选，并用ICP-AES测量每个部分的硼浓度。结果显示，BSH 和 BPA 在 G2/M 期的硼吸收率高于 G0/G1 期。然而，BPA 的差异更为明显。 G2/M : G0/G1 比率随着 BPA 培养时间的变化而降低，从而表明 BPA 摄取的细胞周期依赖性。微剂量测定时应考虑肿瘤细胞吸收硼的这种异质性。

项目成果

Yoshida, F.Matsumura, A., Shibata, Y., Okumura, M., et al.: "Cell cycle dependence of boron uptake in two boron compounds used clinical neutron capture therapy"Cancer Letters. (submitted).

Yoshida, F.Matsumura, A.、Shibata, Y.、Okumura, M.等人：“临床中子捕获疗法中使用的两种硼化合物中硼摄取的细胞周期依赖性”癌症快报。

Matsumura, A., Shibata, Y., Yamamoto, T., Yoshida, F., et al.: "In vivo and in vitro uptake study of boronated porphyrins for neutron capture therapy"Frontiers in Neutron Capture Therapy. 1015-1020 (2001)

Matsumura, A.、Shibata, Y.、Yamamoto, T.、Yoshida, F.等人：“用于中子捕获疗法的硼化卟啉的体内和体外摄取研究”中子捕获疗法的前沿。

Yamamoto, T., Matsumura, A., Shibata, Y., Yoshida, F., et al.: "Apoptosis of tumor cells in response to boron neutron capture therapy"Frontiers in Neutron Capture Therapy. 1293-1297 (2001)

Yamamoto, T.、Matsumura, A.、Shibata, Y.、Yoshida, F.等人：“硼中子捕获疗法响应肿瘤细胞凋亡”中子捕获疗法前沿。

Yoshida,F.,Matsumura,A.et.al: "Cell cycle dependence of boron uptake in varous boron compounds usedfor neutron capture therapy."Ninth International Symposium on neutron capture therapy for cancerProgram and Abstracts. 57-58 (2000)

Yoshida, F., Matsumura, A.等人：“用于中子捕获疗法的各种硼化合物中硼摄取的细胞周期依赖性。”第九届癌症中子捕获疗法国际研讨会计划和摘要。

Yoshida, F., Matsumura, A, Shibata, Y., et al.: "Cell cycle dependence of boron uptake in various boron compounds used for neutron capture therapy"Ninth International Symposium on neutron capture therapy for cancer Program and Abstracts. 57-58 (2000)

Yoshida, F.、Matsumura, A、Shibata, Y.等人：“用于中子捕获疗法的各种硼化合物中硼摄取的细胞周期依赖性”第九届国际癌症中子捕获疗法研讨会计划和摘要。