Boron compound uptake augmentation to use for neutron capture therapy.

硼化合物吸收增强用于中子俘获疗法。

基本信息

批准号：
17591245
负责人：
YOSHIDA Fumiyo
金额：
$ 1.09万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591245/
关键词：
BNCT BSH BSO in vivo

项目摘要

Boron neutron capture therapy (BNCT) is a targeted radiation therapy using a boron (B^<10>) compound and thermal neutrons. Sodium borocaptate (BSH) is widly used for BNCT of brain tumor. We reported that BSH uptake was enhanced in combination with butionine sulfoximine (BSO) in vitro (Cancer Lett. 215 (2004) 61-67). In this study, we investigated influence of BSO on BSH distribution in vivo. 9L glicosarcoma cells were subcutaneously inoculated to right legs of Fisher 344 rats. After 2 weeks, the rats were divided into 3 groups, BSO+ group, BSO-group and untreated group (control). The rats were sacrificed at 3 hours, 6hours or 12 hours after injection, and samples of brain, blood, subcutaneous tumor, muscle, skin, liver and kidney were obtained. The results of the blood boron concentration were 55.2±15.0 μg/mg at 3 hours after injection, 20.2±14. μg/mg at 6 hours, 10.8 ±20.6 μg/mg in BSO+ group. On the other hands, in BSH only group, they were 8.1 ± 6.2 μg/rag, 3.4 ± 0.5 μg/mg, 1.3 ± 0.2 μg/mg, respectively. The boron concentration included in each tissues were in proportion to blood boron concentration.Since in vivo boron level was maintained with high concentration by BSO combination, we performed BNCT for the subcutaneous tumor which we made to the rats legs. As the result, a tumor reduced BSO+ group significantly in comparison with BSO-group.In conclusion, the uptake of BSH was enhanced in combination with BSO in vivo as well as in vitro.

硼中子俘获疗法（BNCT）是一种使用硼（B^10）化合物和热中子钠（BSH）的靶向放射疗法，广泛用于脑肿瘤的BNCT我们报道了BSH的摄取增强。体外使用丁硫酰亚胺 (BSO) (Cancer Lett. 215 (2004) 61-67) 在本研究中，我们进行了研究。 BSO对BSH体内分布的影响将9L胶质肉瘤细胞皮下接种至Fisher 344大鼠的右腿，2周后将大鼠分为3组，BSO+组、BSO-组和未处理组(对照组)。注射后3小时、6小时或12小时处死小鼠，获取脑、血液、皮下肿瘤、肌肉、皮肤、肝脏和肾脏样本。硼浓度注射后3小时为55.2±15.0μg/mg，6小时为20.2±14μg/mg，BSO+组为10.8±20.6μg/mg，而仅BSH组为8.1±20.6μg/mg。 6.2微克/布，3.4±0.5微克/毫克，1.3±0.2微克/毫克，各组织中所含的硼浓度与血液硼浓度成正比。由于BSO组合使体内硼水平维持在高浓度，因此我们对大鼠腿部制作的皮下肿瘤进行了BNCT，结果形成了肿瘤。与BSO-组相比，BSO+组显着减少。总之，在体内和体外，与BSO联合使用的BSH的摄取均得到增强。

项目成果

期刊论文数量（17）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Yoshida F(+5 authors) Combination of boron and gadolinium compounds for neutron capture therapy. An in vitro study.

Yoshida F（5位作者）硼和钆化合物的组合用于中子捕获疗法。

DOI：
发表时间：
2005
期刊：
J.Exp.Clin.Cancer Res. 24
影响因子：
0
作者：
Matsumura A;Yamamoto T;Zhang T
通讯作者：
Zhang T

Matsumura A Excision of juvenile pilocytic astrocytoma of the midbrain after radiotherapy.

Matsumura A 放疗后中脑幼年毛细胞星形细胞瘤的切除术。

DOI：
发表时间：
2006
期刊：
Pediatr.Neurosurg. 42
影响因子：
0
作者：
Tsuboi K;Matsuda W;Nakamura K;Takano S
通讯作者：
Takano S

Combination of Boron and Gadolinium Compounds for Neutron Capture Therapy. An In Vitro Study.

用于中子捕获疗法的硼和钆化合物的组合。

DOI：
发表时间：
2005
期刊：
Clin.Cancer Res. 24
影响因子：
0
作者：
Matsumura A;Zhang T;Nakai K;Endo K;Kumada H;Yamamoto T;Yoshida F;Sakurai F;Yamamoto K;Nose T
通讯作者：
Nose T

Perfusion-weighted magnetic resonance imaging of the spinal cord in cervical spondylotic myelopathy.

脊髓型颈椎病脊髓的灌注加权磁共振成像。