Effects of endocrine disrupter on haploid spermatids and heterozygous mice with germ cell deficiency

内分泌干​​扰物对单倍体精子细胞和生殖细胞缺陷杂合小鼠的影响

• 批准号: 12836007
• 负责人: NOZAKI Masami
• 金额: $ 2.37万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12836007/
• 关键词: Mouse; Testis; Spermatogenesis; Biphenol A; Diethylstilbestrol; Gene expression; Haploid germ cells; ポリカーボネート; 生殖器官; マウス; 精子形成; 合成樹脂; 遺伝子発現

We studied the effects of a Bisphenol A (BPA) on spermratogenesis.1. Young male mice (4 week old ; C57BL/6) were treated orally with 200-2000 ng/kg of BPA three times a week for 6 weeks. External view of organs were obserbed and histological examination of testis were performed. Fertility and morphological view of organs of treated males were not affected with BPA, although a part of testicular germ cells showed necrotic change.2. Young female mice (4 weeks old ; C57BL/6) were treated orally with 20-200 ng/kg of BPA three times a week for 6 weeks. And then the treated females were coupled with males. Male offsprings from such crosses were examined. Testis size of many males were reduced and germ cell defects were clearly obserbed. Moreover, prostate enlarged up to 10 week old.These results indicated that BPA affects on male reproductive physiology.

我们研究了双酚A（BPA）对精子发生的影响1。年轻的雄性小鼠（4周大； C57BL/6）口服治疗200-2000 ng/kg的BPA每周三次，持续6周。观察器官的外观，并对睾丸进行组织学检查。尽管睾丸生殖细胞的一部分表现出坏死的变化，但接受治疗雄性器官的生育能力和形态学不受BPA的影响。2。年轻的雌性小鼠（4周大; C57BL/6）用20-200 ng/kg的BPA口服治疗，每周3次，持续6周。然后将治疗的女性与雄性结合。检查了这些十字架的男性后代。许多雄性的睾丸大小降低，生殖细胞缺陷清楚地观察到。此外，前列腺扩大了10周大。这些结果表明BPA会影响男性生殖生理学。

项目成果

Masahito Ikawa: "Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertilty"Dev. Biol.. 240. 254-261 (2001)

Masahito Ikawa：“Calmegin 是生育素 α/β 异二聚化和精子受精所必需的”Dev.

Hiromitsu Tanaka: "Cloning and characterization of a human orthologue of testis-specific succinyl CoA : 3-oxo acid CoA transferase (Scot-t) cDNA"Mol Hum Reprod. 8. 16-23 (2001)

Hiromitsu Tanaka：“睾丸特异性琥珀酰 CoA 的人类直系同源物的克隆和表征：3-含氧酸 CoA 转移酶 (Scot-t) cDNA”Mol Hum Reprod。

Yasuhiro Tosaka: "Identification and characterization of testis specific ornithine decarboxylase antizyme (OAZ-t) gene : expression in haploid germ cells and polyamine-induced frames hifting"Genes Cells. 5. 265-276 (2000)

Yasuhiro Tosaka：“睾丸特异性鸟氨酸脱羧酶抗酶 (OAZ-t) 基因的鉴定和表征：单倍体生殖细胞中的表达和多胺诱导的框架移位”基因细胞。

Shuji Takabayashi: "The ter/ter gonadal somatic cells cause apoptosis in ter/ter primordial germ cells (PGCs) with normal survivability and proliferation ability in the mouse : evidence from PGC-somatic cell "exchange-co-culture""Zool. Sci.. 18. 695-704 (

Shuji Takabayashi：“在小鼠中，ter/ter性腺体细胞导致具有正常生存能力和增殖能力的ter/ter原始生殖细胞（PGC）凋亡：来自PGC-体细胞“交换共培养”的证据“Zool.

Y. Tosaka: "Identification and characterization of testis specific ornithine decarboxylase antizyme (OAZ-t) gene : Expression in haploid germ cells and polyamine-induced frameshifting"Genes Cells. 5. 265-276 (2000)

Y. Tosaka：“睾丸特异性鸟氨酸脱羧酶抗酶 (OAZ-t) 基因的鉴定和表征：在单倍体生殖细胞中的表达和多胺诱导的移码”Genes Cells。